Fulvic Acid: A Tool for Controlling Powdery and Downy Mildews in Cucumber Plants

Said M. Kamel, Mohamed M.I. Afifi, Fathia S. El-shoraky, Mohamad M. El-Sawy

Abstract


A greenhouse experiment was carried out on cucumber cultivar DP-164 during the seasons 2012-2013 to evaluate the efficacy of three concentrations of fulvic acid (FA) to control downy and powdery mildew diseases compared with the recommended fungicides and their effects on plant growth, chlorophyll content and yield of cucumber plants. Results revealed that all FA concentrations significantly reduced disease severity of both diseases. The highest reduction in disease severity of downy mildew was recorded using 75 ppm of FA, which was more effective than the recommended fungicide. The significant effect of inhibition of powdery mildew diseases increased gradually with increased FA concentration. At the same time, the reduction of diseases severity was greater than or equal to the recommended fungicides. Foliar application of FA concentrations and Ridomil fungicide increased significantly all morphological characters. Sumi-8 fungicide increased the morphological characters except plant height, dry weight/ plant and leaves number/ plant. The yield components (weight of fruits and mean of fruit weight /plant) recorded the same values with all FA concentrations. Fulvic acid had direct effect on soil microbiology by increasing plant exudates, which enhanced the activity of microorganism. Fulvic acid at 150ppm showed the highest increase in dehydrogenase and nitrogenase activity. This study revealed that the foliar application of FA improved plant growth and yield quantity and quality as well as controlling powdery and downy mildews of cucumber plants. Additionally, FA has the advantage as effective and environmental friendly agent 

Keywords


Control; humic substance; Podosphaera fusca; Pseudoperonospora cubensis

References


Abdel-Kareem, F. 2007. Induced resistance in bean plants against root rot and Alternaria leaf spot diseases using biotic and a biotic inducers under field conditions. Journal of Agricultural and Biological Sciences 3: 767 – 774.

Achuo, E.A., K.Audenaert, H.Meziana, M.Hofte.2004. The salicylic acid dependent defence pathway is effective against different fungi in tomato and tobacco. Plant Pathology 53: 65 – 72.

Afifi, M.M.I. 2010. Biochemical studies on humic substances extracted from organic fertilizers. Ph.D. Diss, Fac. Agric., Cairo Univ., Egypt.

Alvarez, E., C.Grajales, J. Villegas, J.Loke. 2002. CIAT Informe Anual. Control del mildeo polvoso (Sphaerotheca panosa var. rosae) en rosa, usando un lixiviado de compost del raquis de plátano (Musa AAB). (Available online with updates at http://www.ciat.cgiar.org/ipm/pdfs/cassava%20_ Pathology.pdf).

American Public Health Association (APHA).1989. Municipal Refuse Disposal 3rd Ed. Public AdministrationService, Chicago, USA.

BioAg Technologies International. 1999. Humid acid structure and properties. Available Online at: Last Accessed: September 11, 2006. Ag Technologies International at 916: 371-694. (Available online with updates athttp://www.phelpstekcom/ graphics/bioag/humic acid. Pdf).

Black, C.A., D.O.Evans's, L.E.Ensmunger, J.L.White, F.E.Clark, R.C.Dineure. 1965. Methods of Soil Analysis II, Chemical and Microbiological Properties. American Soc. Argon. Madison, Wisconsin, USA.

Brunelli, A., R.Davi. 1987. Peronospora disease of cucurbits. Informator Fitipatologico 37: 17-20.

Canellas, L.P., F.L.Olivares, A.L.Okorokova-Facanha, A.R.Facanha. 2002. Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence and plasma membrane H-ATPase activity in maize roots. Plant Physiology l30: 1951-1957.

Chapman, H.D., F.P.Pratt. 1961. Methods of Analysis for Soils, Plants and Water. Univ. California, Div. Agric., Sci. Riverside, USA. No. 309 p.

Chen, Y., C.Clapp, E.Magen.2004a. Mechanisms of plant growth stimulation by humic substances: The role of organo-iron complexes. Soil Science and Plant Nutrition 50: 1089-1095.

Chen, Y., M.D.Nobili, T.Aviad. 2004b. Stimulatory Effect of Humic Substances on Plant Growth. In: Magdoft. F. and R. Ray (Eds.): Soil Organic Matter in Sustainable Agriculture, CRC Press, Washington DC. pp 103.

De Kreij, C., H.Basar.1995. Effect of humic substances in nutrient film technique on nutrient uptake. Journal of Plant Nutrition 18: 793-802.

Derbalah, A. S., S. Z. Morsy, S. M. kamel and M. M. El-Sawy, 2012. Recent Approaches towards Controlling Powdery Mildew of Pepper under Greenhouse Conditions. Egyptian Journal of Biological Pest Control, 22(2): 205-210.

Dmitrier, A., M.Tena, J.Jorrin. 2003. Systemic acquired resistance in sunflower (Helianthus annuusL.). Tsitologiya-1-Genetika 37: 9-15.

Dragunova, A.F.1958. A rapid method for determining functional groups in humic acids. Nauch Trudy, Mosk. I in Zh. Chonon Inst. Ser. Khinprioz-vod., Cited by Kononova (1966), 544 p.

El-Ghamry, A.M., K.M.Abd El-Hai, K.M.Ghoneem.2009. Amino and humic acids promote growth, yield and disease resistance of faba bean cultivated in clayey soil. Australian Journal of Basic and Applied Sciences 3: 731-739.

Elisabetta, L., B. Mariagrazia,S.Nicola. 2008. The role of humic fractions from soil and compost in controlling the growth in vitro of phytopathogenic and antagonistic soil-borne fungi. Ecotoxicology and Environmental Safety 69: 350 – 357.

Escobar Velez, J.H., J.Castaño Zapata. 2005. Fulvic acid applications for the management of diseases caused by Mycosphaerella spp. InfoMusa 14:15-17.

Fahri, Y, D.S. Murat.2008. Effect of Humic Acid Applications on the Root-Rot Diseases Caused by Fusarium spp. on Tomato Plants. Plant Pathology Journal 7: 179-182.

Frgbenro, J.A., A.A.Agboola.1993. Effect of different levels of humic acid on growth and nutrient uptake of teak seedlings. Journal of Plant Nutrition 16: 1465-1483.

Goh, K.M., F.J.Stevenson.1971. Comparison of infra-red spectra of synthetic and neutral humic and fulvic acids. Soil Science 112: 392-400.

Hahlbrock, K., D.Scheel.1989. Physiology and molecular biology of phenyl propanoid metabolism. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40: 347-369.

Iannotti, D.A., T. Pang,B.L.Troth, D.L.Elwell, H.M.Keener, H.A.J.Hoitink.1993. A quantitative respirometric method for monitoring compost stability. Compost Science and Utilization 1:52-65.

IHSS. 2004. International Humic Substances Society. Isolation of Samples, IHSS Vol. 2006. (Available online with updatesat http://www.ihss.gatech.edu, on-line 8.1.2006).

Kauser, A.M., F.Azam. 1985. Effect of humic acid on wheat (Triticum aestivum L.) seedling growth. Environ. Exp. Bot. 25: 245-252.

Kononova, M.M. 1966. Soil Organic Matter. Pergmon Press, Oxford, London, Edinburgh, New York, 544 p.

MacCarthy, P., C.E.Clapp, R.L.Malcolm, R.Bloom. 1990. Humic substances in soil and crop scienes: Selected Readings, American Society of Agronomy, Madison, Wisconsin.Mackinney, G., 1941.

Mackowiak, C.L., P.R.Gross, B.G.Bugbee.2001. Beneficial effects of humic acid on micronutrient availability to wheat. Soil Science Society American Journal 56: 1744-1750.

Maggioni, A., Z. Varanini,S.Nardi, R.Finton.1987. Action of soil humic matter on plant roots: Stimulation of ion uptake and effects on (Mg'.+K) ATPase activity. Sci. Tot. Environ 62: 355-363.

Murphy, J., J.P.Riley. 1962. A modified single solution method for the determination of phosphatic in natural water. Anal. Chem. Acta 27:31-36.

Neil, B. 2012. Fulvic acid- the natural antifungal. Available online from http://www.ezinearticles. com/?expert=Neil_Butterfield.

Piccolo, A., S. Nardi,G.Concheri.1992. Structural characteristics of humic substances as regulated to nitrate uptake and growth regulation in plant systems. Soil Biochem 24: 373-380.

Russo, R.O., G.P.Borlyn. 1990. The use organic biostimulants to help low input sustainable agriculture. J. Sust. Agric 1: 19-42.

Sánchez Rodríguez, R., J.A.Pino Algora, C.Vallin Plous,M.E.Pérez Rodríguez, Y.Iznaga Sosa, F. Malpartida Romero.2002. Effects of the natural fungicide F20 on black Sigatoka disease (Mycosphaerella fijiensis Morelet) on plantain (AAB) and banana (AAA). IN FOM USA 11:14-16.

Scheuerell, S.J., W.H.Mahaffee.2004. Compost Tea as a Container Medium Drench for Suppressing Seedling Damping-Off Caused by Pythium ultimum. Phytopathology 94: 1156-1163.

Scheuerell, S.J., W.H.Mahaffee.2006. Variability Associated with Suppression of Gray Mold (Botrytis cinerea) on Geranium by Foliar Applications of Nonaerated and Aerated Compost Teas. Plant Disease 90: 1201-1208.

Schnitzer, M., U.C.Gupta. 1965. Determination of acidity in soil organic matter. In: (Schnitzer, M. and Khan, S.U. (eds): Soil Organic Matter. Soil Sci. Soc. Amer. Proc 29: 274 – 277.

Sherry, L., C. Murray,G.Ramage. 2011. Carbohydrate derived fulvic acid (CHD-FA) is a novel antifungal product category: Front Microbiol 3: 116.

Sitterly, W.R. 1978. Powdery mildew of cucurbits. In: Spencer, D. M. (ed): The powdery mildews. Academic press, New York, San Francisco, pp 359- 379.

Steel, R.G.D., I.H.Torrie. 1980. Principles and procedures of statistics. McGraw Hill Book Company, New York, USA .

Stevenson, F.J. 1994. Humus Chemistry: Genesis, Composition, Reaction (Second Ed.), Wiley, New York, USA.

TMECC. 2002. Test methods for the examination of composting and composts. ed. Wayne Thompson. The US Composting Council. US Government printing office.

Ulukon, H. 2008. Effect of soil applied humic acid at different sowing times on some yield components in wheat (Triticum spp.) hybrids Int. J. Bot. 4: 164-175.

Vaughan, D., R.E.Malcolin. 1979. Effect of humic acid on invertase synthesis in roots of higher plants. Soil Biol. Biochem.11: 247-252.

Velez, J.H.E., J.C.Zapata. 2005. Fulvic acid applications for the management of diseases caused by Mycosphaerella spp. InfoMusa 14: 15-17.

Weltzien, H.C. 1992. Biocontrol of foliar fungal diseases with compost extracts. In: J.H. Andrews and S.S. Hirano (Eds.): Microbial Ecology of Leaves. Springer-VerlagPublishers, New York, USA.

Yadava, U.L. 1986. A rapid and non destructive method to determine chlorophyll in intact leaves. Hort. Sci. 21: 1449.

Yohalem, D.S., R.F.Harres, J.H.Andrews. 1994. Aqueous extracts of spent mushroom substrate for foliar disease control. Compost Sci. Util. 2: 67-74.

Zhang, X. 1997. Influence of plant growth regulators on turfgrasss growth, antioxidant status, and drought tolerance. Ph.D. Diss., Fac. of Virginia Polytechni. (Institute and State University).

Ziadi, S, S.Barbedette, J.F.Godard, C.Monoti, L.E.D.Corre, D.Silue, D.Lecorre. 2001 Production of pathogenesis related protein in the cauliflower (Brassica oleacea var. botrytis) downy mildew (Peronospora parasistica) pathosystem treated with acidbenzolar-5-methyl. Plant Pathology 50: 579-586.

Zucconi, F., M.de Bertoldi.1987. Compost specification for the production and characterisation of compost from municipal solid waste. In: M. de Bertoldi, M.P. Ferranti, P. L’Hermite, F. Zucconi (Eds:): Compost, production, Quality and Use. Elsevier Applied Science. pp 30-50.


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DOI: 10.33687/phytopath.003.02.0866

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Copyright (c) 2014 Said M. Kamel, Mohamed M.I. Afifi, Fathia S. El-shoraky, Mohamad M. El-Sawy

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