Greenhouse-Grown Tomatoes: Microbial Diseases and their Control Methods: A Review

Nooreen M. Ally, Hudaa Neetoo, Vijayanti M. Ranghoo-Sanmukhiya, Teresa A. Coutinho


The cultivation of tomatoes in greenhouse structures is becoming increasingly common as a crop production system. However, the environmental conditions inside a greenhouse favour the development of microbial diseases. These diseases have an adverse effect on the tomato yield and can lead to serious economic losses. This review will give an insight into the major diseases affecting greenhouse-grown tomatoes, the respective causal agents and recommended control strategies. Some of the major diseases are of a bacterial, fungal, viral or nematode origin and include bacterial spot and speck, bacterial canker, early blight, gray mold, leaf mold, powdery mildew, tomato mosaic virus, tomato big bug and root knot. For each disease, the symptoms and risk factors responsible for disease development are described. In addition, the different structural designs and set-ups of tomato greenhouses are covered along with their advantages and limitations, especially any adverse effects on yield and susceptibility to disease. Although conventional control measures are discussed, greater emphasis is laid on the use of alternative biocontrol measures that include Effective Microorganisms, natural antimicrobials and nanobiopesticides. Moreover, information gathered in this review is based on a combination of available literature and expert guidance. This compilation is hoped to be instructive for tomato growers opting for greenhouse farming and assist them in the application of timely and more effective control measures


Biocontrol; Diseases; Greenhouses; Nanoparticles; Pathogens; Tomato


Abo-Elyousr, K. A., H. M. Khalil Bagy, M. Hashem, S. A. Alamri and Y. S. Mostafa. 2019. Biological control of the tomato wilt caused by Clavibacter michiganensis subsp. michiganensis using formulated plant growth-promoting bacteria. Egyptian Journal of Biological Pest Control, 29: 1-8.

Ahmed, R. 2022. First report of bacterial speck of tomato caused by Pseudomonas syringae pv. tomato in AJK, Pakistan. Planta Animalia, 1: 31-32.

Ahmed, R., A. S. Gondal, M. T. Khan, S. Shahzaman and S. Hyder. 2018. First report of Botrytis cinerea causing gray mold disease on peach from Pakistan. International Journal of Phytopathology, 7: 131-31.

Ajdari, Z., H. Rahman, K. Shameli, R. Abdullah, M. Abd Ghani, S. Yeap, S. Abbasiliasi, D. Ajdari and A. Ariff. 2016. Novel gold nanoparticles reduced by Sargassum glaucescens: Preparation, characterization and anticancer activity. Molecules, 21: 123.

Akther, T. and S. Hemalatha. 2019. Mycosilver nanoparticles: Synthesis, characterization and its efficacy against plant pathogenic fungi. BioNanoScience, 9: 296-301.

Ali, E. O. M., N. A. Shakil, V. S. Rana, D. J. Sarkar, S. Majumder, P. Kaushik, B. B. Singh and J. Kumar. 2017. Antifungal activity of nano emulsions of neem and citronella oils against phytopathogenic fungi, Rhizoctonia solani and Sclerotium rolfsii. Industrial Crops and products, 108: 379-87.

Alsanius, B. W., M. Karlsson, A. K. Rosberg, M. Dorais, M. T. Naznin, S. Khalil and K.-J. Bergstrand. 2019. Light and microbial lifestyle: The impact of light quality on plant–microbe interactions in horticultural production systems-A review. Horticulturae, 5: 41.

Bale, J., J. Van Lenteren and F. Bigler. 2008. Biological control and sustainable food production. Philosophical Transactions of the Royal Society B: Biological Sciences, 363: 761-76.

Bartok, J. W. 2013. Plastic Greenhouse Film Update. University of Massachusetts. Amherst.

Bashir, A., M. T. Khan, R. Ahmed, B. Mehmood, M. T. Younas, H. M. Rehman and S. Hussain. 2020. Efficiency of selected botanicals against (Alternaria solani) causing early blight disease on tomato in Azad Jammu and Kashmir. Pakistan Journal of Phytopathology, 32: 179-86.

Bjarnadottir, A. 2019. Tomatoes 101. Healthline Media. United States.

Blancard, D. 2012. Tomato diseases: Identification, biology and control: A colour handbook. CRC Press: Florida, USA.

Bonanomi, G., V. Antignani, M. Capodilupo and F. Scala. 2010. Identifying the characteristics of organic soil amendments that suppress soilborne plant diseases. Soil Biology and Biochemistry, 42: 136-44.

Branthôme, F. X. 2020. Global Trade and Consumption of Tomato Products in 2019/2020.

Burrows, R. 2019. Tomato Spotted Wilt Virus. South Dakota State University. Brookings, Washington, D.C., United States.

Campolo, O., A. Cherif, M. Ricupero, G. Siscaro, K. Grissa-Lebdi, A. Russo, L. M. Cucci, P. Di Pietro, C. Satriano and N. Desneux. 2017. Citrus peel essential oil nanoformulations to control the tomato borer, Tuta absoluta: Chemical properties and biological activity. Scientific Reports, 7: 13036.

Campos, E. V., P. L. Proença, J. L. Oliveira, M. Bakshi, P. Abhilash and L. F. Fraceto. 2019. Use of botanical insecticides for sustainable agriculture: Future perspectives. Ecological indicators, 105: 483-95.

Chen, Y., F. Yan, Y. Chai, H. Liu, R. Kolter, R. Losick and J. h. Guo. 2013. Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation. Environmental microbiology, 15: 848-64.

Chin, A. and S. A. Miller. 2010. Tomato Mosaic Virus. The Ohio State University, The Department of Plant Pathology. Wooster, OH, United States.

Dalai, S., B. Tripathy, S. Mohanta, B. Sahu and J. B. Palai. 2020. Green-houses: Types and structural components. In, Protected Cultivation and Smart Agriculture. New Delhi Publishers. New Delhi, India.

Dannehl, D., H.-P. Kläring and U. Schmidt. 2021. Light-mediated reduction in photosynthesis in closed greenhouses can be compensated for by CO2 enrichment in tomato production. Plants, 10: 2808.

de Oliveira, J. L. 2021. Nano-biopesticides: Present concepts and future perspectives in integrated pest management. In, Advances in nano-fertilizers and nano-pesticides in agriculture. Woodhead Publishing. United Kingdom.

Droby, S. and A. Lichter. 2007. Post-harvest Botrytis infection: Etiology, development and management. In, Botrytis: Biology, pathology and control. Springer. Dordrecht.

Duford, M. J. 2022. Types of tomatoes: A comprehensive category guide to shapes, sizes, heirlooms, and more.

Elad, Y., B. Williamson, P. Tudzynski and N. Delen. 2007. Botrytis spp. and diseases they cause in agricultural systems–An introduction. In, Botrytis: Biology, pathology and control. Springer. Dordrecht, The Netherlands.

FAO. 2021. World Food and Agriculture-Statistical Yearbook 2021. Food and Agriculture Organization. Rome, Italy.

Fillhart, R. C., G. D. Bachand and J. D. Castello. 1998. Detection of infectious tobamoviruses in forest soils. Applied and Environmental Microbiology, 64: 1430-35.

Gan, P. P., S. H. Ng, Y. Huang and S. F. Y. Li. 2012. Green synthesis of gold nanoparticles using palm oil mill effluent (POME): A low-cost and eco-friendly viable approach. Bioresource Technology, 113: 132-35.

Gannibal, P. B., A. S. Orina, N. V. Mironenko and M. M. Levitin. 2014. Differentiation of the closely related species, Alternaria solani and A. tomatophila, by molecular and morphological features and aggressiveness. European journal of plant pathology, 139: 609-23.

Gardening Channel. 2021. Mastering common tomato problems.

Garibaldi, A., D. Ricauda Aimonino, I. Luongo, G. Gilardi and M. Gullino. 2014. Different steaming methods to control Fusarium wilt agents under simulated conditions. VIII International Symposium on Chemical and Non-Chemical Soil and Substrate Disinfestation 1044.

Gebhardt, C. 2016. The historical role of species from the Solanaceae plant family in genetic research. Theoretical and Applied Genetics, 129: 2281-94.

Grabowski, M. 2018. Bacterial canker of tomato. University of Minnesota. United States.

Grasso, G., D. Zane and R. Dragone. 2019. Microbial nanotechnology: challenges and prospects for green biocatalytic synthesis of nanoscale materials for sensoristic and biomedical applications. Nanomaterials, 10: 11.

Guan, W. 2017. Control root-knot nematode in high tunnels. Purdue University. West Lafayette, United States.

Holsteens, K., R. Moerkens, B. Van de Poel and W. Vanlommel. 2020. The effect of low-haze diffuse glass on greenhouse tomato and bell pepper production and light distribution properties. Plants, 9: 806.

Iddio, E., L. Wang, Y. Thomas, G. McMorrow and A. Denzer. 2020. Energy efficient operation and modeling for greenhouses: A literature review. Renewable and Sustainable Energy Reviews, 117: 109480.

Idris, A. 2006. Economic comparison of tomatoes production under green houses versus open fields in Khartoum state, University of Khartoum.

Jackson, G. 2017. Tomato big bud. Australian Centre for International Agricultural Research. Canberra, ACT, Australia.

Jones, C., J. Jones and W. Lee. 2010. Diagnosis of bacterial spot of tomato using spectral signatures. Computers and electronics in agriculture, 74: 329-35.

Jones, H. E., J. M. Whipps, B. J. Thomas, T. L. Carver and S. J. Gurr. 2000. Initial events in the colonisation of tomatoes by Oidium lycopersici, a distinct powdery mildew fungus of Lycopersicon species. Canadian Journal of Botany, 78: 1361-66.

Jones, J. B., G. E. Vallad, F. B. Iriarte, A. Obradović, M. H. Wernsing, L. E. Jackson, B. Balogh, J. C. Hong and M. T. Momol. 2012. Considerations for using bacteriophages for plant disease control. Bacteriophage, 2: e23857.

Jones, J. B., T. A. Zitter, T. M. Momol and S. A. Miller. 2016. Compendium of Tomato Diseases and Pests. American Phytopathological Society: St. Paul, USA.

Joshi, H., C. P. Somduttand and S. Mundra. 2019. Role of effective microorganisms (EM) in sustainable agriculture. International Journal of Current Microbiology and Applied Sciences, 8: 172-81.

Karanisa, T., Y. Achour, A. Ouammi and S. Sayadi. 2022. Smart greenhouses as the path towards precision agriculture in the food-energy and water nexus: Case study of Qatar. Environment Systems and Decisions, 42: 521-46.

Kaushik, N., C. E. Díaz, H. Chhipa, L. F. Julio, M. F. Andrés and A. González-Coloma. 2020. Chemical composition of an aphid antifeedant extract from an endophytic fungus, Trichoderma sp. EFI671. Microorganisms, 8: 420.

Khalifa, E. 1991. Biological control of tomato fusarium wilt by Trichoderma harzianum. Minufiya Journal of Agricultural Research, 16: 1247-59.

Khan, I. 2018. Top 10 tomato producing countries.

King, E. O., M. K. Ward and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanin and fluorescin. The Journal of laboratory and clinical medicine, 44: 301-07.

Kolomiets, J., I. Grygoryuk and L. Butsenko. 2017. Bacterial diseases of tomato plants in terms of open and covered growing of Ukraine. Annals of Agrarian Science, 15: 213-16.

Kubota, C., A. Gelder and M. Peet. 2018. Greenhouse tomato production. In, Tomatoes. CABI Publishing. Wallingford, UK.

Kumari, M., S. Pandey, A. Bhattacharya, A. Mishra and C. Nautiyal. 2017. Protective role of biosynthesized silver nanoparticles against early blight disease in Solanum lycopersicum. Plant Physiology and Biochemistry, 121: 216-25.

Lade, B. D. and D. P. Gogle. 2019. Nano-biopesticides: Synthesis and applications in plant safety. In, Nanobiotechnology Applications in Plant Protection. Springer, Cham.

Leonardi, C., P. Ambrosino, F. Esposito and V. Fogliano. 2000. Antioxidative activity and carotenoid and tomatine contents in different typologies of fresh consumption tomatoes. Journal of Agricultural and Food Chemistry, 48: 4723-27.

Li, R., S. Gao, Z. Fei and K.-S. Ling. 2013. Complete genome sequence of a new tobamovirus naturally infecting tomatoes in Mexico. Genome announcements, 1: e00794-13.

Li, Y. 2020. Gray mold of tomato. The Connecticut Agricultural Experiment Station. New Haven, CT.

Liao, Y., A. Strayer-Scherer, J. White, R. De La Torre-Roche, L. Ritchie, J. Colee, G. Vallad, J. Freeman, J. Jones and M. Paret. 2019. Particle-size dependent bactericidal activity of magnesium oxide against Xanthomonas perforans and bacterial spot of tomato. Scientific Reports, 9: 1-10.

Lippi, G. and G. Targher. 2011. Tomatoes, lycopene-containing foods and cancer risk. British Journal of Cancer, 104: 1234-35.

Litterick, A., L. Harrier, P. Wallace, C. Watson and M. Wood. 2004. The role of uncomposted materials, composts, manures, and compost extracts in reducing pest and disease incidence and severity in sustainable temperate agricultural and horticultural crop production-A review. Critical reviews in plant sciences, 23: 453-79.

Mackenzie, K., J. Chitwood, G. Vallad and S. Hutton. 2018. Target spot of tomato in Florida. University of Florida, Institute of Food and Agricultural Sciences. United States.

Mamode Ally, N., H. Neetoo, M. Ranghoo-Sanmukhiya, S. Hardowar, V. Vally, A. Gungoosingh-Bunwaree, T. A. Coutinho, M. Vojvodić and A. Bulajić. 2021. First report of target spot on tomato caused by Corynespora cassiicola in Mauritius. Plant Disease, 105: 226.

Maraveas, C. 2020. The sustainability of plastic nets in agriculture. Sustainability, 12: 3625.

Marks, D. 2010. Identify, prevent, treat Tobacco mosaic virus. GardenFocused. United Kingdom.

Maudarbaccus, F., K. Lobin, V. Vally, A. Gungoosingh‐Bunwaree and W. Menzel. 2021. First report of Tomato mottle mosaic virus on tomato in Mauritius. New Disease Reports, 44: 1-2.

McGrath, M. 2021. Bacterial canker of tomato. Cornell University, College of Agriculture and Life Sciences. New York, United States.

Mellifiq. 2020. Efficient water treatment and reuse in greenhouses. Hägersten, Sweden.

Meuldijk, M. 2020. How do you choose the right ground cover?

———. 2021. How to steam soil. Royal brinkman.

Mishra, A., M. Kumari, S. Pandey, V. Chaudhry, K. Gupta and C. Nautiyal. 2014. Biocatalytic and antimicrobial activities of gold nanoparticles synthesized by Trichoderma sp. Bioresource Technology, 166: 235-42.

Mittal, D., G. Kaur, P. Singh, K. Yadav and S. Ali. 2020. Nanoparticle-based sustainable agriculture and food science: Recent advances and future outlook. Frontiers in Nanotechnology, 2: 579954.

Murthy, K. S., V. Vineela and P. Vimala Devi. 2014. Generation of nanoparticles from technical powder of the insecticidal bacterium Bacillus thuringiensis var. kurstaki for improving efficacy. International Journal of Biomedical Nanoscience and Nanotechnology, 3: 236-50.

Nag, O. S. 2020. The world's leading tomato producing countries.

Naika, S., J. de Jeude, M. de Goffau, M. Hilmi and B. van Dam. 2019. Cultivation of tomato production, processing and marketing. Agromisa Foundation and CTA: Wageningen.

Nandi, M., J. Macdonald, P. Liu, B. Weselowski and Z. C. Yuan. 2018. Clavibacter michiganensis ssp. michiganensis: Bacterial canker of tomato, molecular interactions and disease management. Molecular plant pathology, 19: 2036-50.

Nederhoff, E. and L. Marcelis. 2010. Calculating Light and Lighting. Practical Hydroponics and Greenhouses: 43-51.

Newell, P. 2003. PESTS| Nematodes and Mollusks. Encyclopedia of Applied Plant Sciences: 665–72.

Nicolopoulou-Stamati, P., S. Maipas, C. Kotampasi, P. Stamatis and L. Hens. 2016. Chemical pesticides and human health: The urgent need for a new concept in agriculture. Frontiers in public health, 4: 148.

Noble, R. and E. Coventry. 2005. Suppression of soil-borne plant diseases with composts: A review. Biocontrol Science and Technology, 15: 3-20.

Nowicki, M., M. R. Foolad, M. Nowakowska and E. U. Kozik. 2012. Potato and tomato late blight caused by Phytophthora infestans: An overview of pathology and resistance breeding. Plant Disease, 96: 4-17.

NPPO of the Netherlands. 2021. Tomato apical stunt viroid (TASVDO).

Olmedo-Velarde, A., R. Hamasaki, B. Bushe and M. Melzer. 2017. Tomato chlorotic dwarf viroid. Plant Disease, 113: 1-3.

Onofre, R. B., D. M. Gadoury, A. Stensvand, A. Bierman, M. Rea and N. A. Peres. 2021. Use of ultraviolet light to suppress powdery mildew in strawberry fruit production fields. Plant Disease, 105: 2402-09.

Özkara, A., D. Akyıl and M. Konuk. 2015. Pesticides, environmental pollution, and health. In, Environmental Health Risk. Intech Open Limited. London.

Pilkington, L. J., G. Messelink, J. C. van Lenteren and K. Le Mottee. 2010. “Protected Biological Control"-Biological pest management in the greenhouse industry. Biological Control, 52: 216-20.

Sabaratnam, S. 2018. Bacterial canker of greenhouse tomato.

Sahayaraj, K. 2014. Nanotechnology and plant biopesticides: An overview. In, Advances in Plant Biopesticides. Springer. New Delhi.

Santhoshkumar, J., S. Rajeshkumar and S. V. Kumar. 2017. Phyto-assisted synthesis, characterization and applications of gold nanoparticles: A review. Biochemistry and biophysics reports, 11: 46-57.

Sathishkumar, M., K. Sneha and Y.-S. Yun. 2010. Immobilization of silver nanoparticles synthesized using Curcuma longa tuber powder and extract on cotton cloth for bactericidal activity. Bioresource Technology, 101: 7958-65.

Scherer, A. and I. Meadows. 2020. Foliar Fungal Diseases on High Tunnel and Greenhouse Tomatoes. NC State Extension Publications: North Carolina, USA.

Seethapathy, P. 2018. Tomato big bud (Candidatus Phytoplasma asteris-related strain BB).

Senthilkumar, P., L. Surendran, B. Sudhagar and D. Ranjith Santhosh Kumar. 2019. Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological potential. SN Applied Sciences, 1: 1-12.

Shafie, R. M., A. M. Salama and K. Y. Farroh. 2018. Silver nanoparticles activity against Tomato spotted wilt virus. Middle East Journal of Agriculture Research, 7: 1251-67.

Shakoor, S., M. Inam-ul-Haq, S. Bibi and R. Ahmed. 2015. Influence of root inoculations with vasicular arbuscular mycorrhizae and rhizomyx for the management of root rot of chickpea. Pakistan Journal of Phytopathology, 27: 153-58.

Sherf, A. F. 2019. Big Bud on Tomato Fruit.

Simko, I., S. Jansky, S. Stephenson and D. Spooner. 2007. Genetics of resistance to pests and disease. In, Potato Biology and Biotechnology. Elsevier Science B.V.

Singh, V. K. and P. Pandey. 2012. Physical methods in management of plant diseases. In, Eco-friendly Innovative Approaches in Plant Disease Management. International Book Distributors and Publisher. New Delhi, India.

Singh, V. K., A. K. Singh and A. Kumar. 2017. Disease management of tomato through PGPB: Current trends and future perspective. 3 Biotech, 7: 1-10.

Sundin, G. W., L. F. Castiblanco, X. Yuan, Q. Zeng and C. H. Yang. 2016. Bacterial disease management: challenges, experience, innovation and future prospects: Challenges in bacterial molecular plant pathology. Molecular plant pathology, 17: 1506-18.

Tariq-Khan, M., A.-D. A. Khan, M. Saeed, S. Z. Gardazi, B. Mehmood, M. Ilyas and R. Ahmed. 2020. Distribution and virulence of root-knot nematodes on summer vegetables in sudhnuti district of azad jammu and kashmir. Pakistan Journal of Phytopathology, 32: 257-64.

Teitel, M., H. Vitoshkin, F. Geoola, S. Karlsson and N. Stahl. 2017. Greenhouse and screenhouse cover materials: Literature review and industry perspective. International Symposium on New Technologies for Environment Control, Energy-Saving and Crop Production in Greenhouse and Plant.

Thompson, S. 2019. UV light may be ripe to replace chemicals in fungus fight. Cornell Chronicle, Ithaca. New York, USA.

Tokeshi, H., M. Aloes, A. Sanches and D. Harada. 1998. Effective Microorganisms for controlling the phytopathogenic fungus Sclerotinia sclerotiorum in lettuce. Proceedings of the 4th International Conference on Kyusei Nature Farming, Bellingham-Washington, USA.

Toor, R., G. Savage and C. Lister. 2006. Seasonal variations in the antioxidant composition of greenhouse grown tomatoes. Journal of Food Composition and Analysis, 19: 1-10.

Vimala Devi, P. S., P. Duraimurugan and K. S. V. P. Chandrika. 2019. Bacillus thuringiensis-based nanopesticides for crop protection. In, NanoBiopesticides Today and Future Perspectives. Academic Press. London, Cambridge.

Von Zabeltitz, C. 2011. Greenhouse structures. In, Integrated Greenhouse Systems for Mild Climates. Springer. Berlin, Heidelberg.

West, J. S., J. A. Townsend, M. Stevens and B. D. Fitt. 2012. Comparative biology of different plant pathogens to estimate effects of climate change on crop diseases in Europe. European journal of plant pathology, 133: 315-31.

Zitter, T. A. and J. F. Murphy. 2009. Cucumber mosaic. The Plant Health Instructor, 9: 512.

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DOI: 10.33687/phytopath.012.01.4273


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