Chemical Management of Alternaria Leaf Blight of Sunflower

Hafiz M. Saqib, Muhammad Abid, Sobia Chohan


Sunflower is an important oilseed crop of Pakistan, comprising 20% proteins and 38-45% oil contents. Alternaria leaf blight (ALB) caused by Alternaria alternata, is one of the devastating diseases of sunflower. Six different fungicides viz., difenoconazole, hexaconazole, azoxystrobin, dimethomorph + mancozeb, myclobutanil and Sulphur were tested at different concentrations (10, 20, 30 ppm) in the laboratory and the greenhouse. Fungicides performed best in the laboratory were also investigated in greenhouse against Alternaria leaf blight of sunflower. In in vitro study, Hexaconazole showed 100 % growth inhibition of A. alternata at 30 ppm followed by 70% at 20 ppm and 62% at 10 ppm. Difenoconazole proved as the 2nd best fungicide with 77% fungal inhibition at 30 ppm, 75.8% at 20 ppm and 71% at 10 ppm. Azoxystrobin was the least effective fungicide with 24%, 28%, 34% fungal inhibition at 10, 20 and 30 ppm, respectively. Twelve cultivars of sunflower were screened against blight disease in pot experiment to check the fungicides at a different level of susceptibility in the greenhouse. Screening result showed that FH 702 was the highest susceptible variety with mean value 7.6. Greenhouse study of disease inhibition effect of fungicides also showed that hexaconazole fungicide produced the best results against A. alternata with 42% disease reduction in FH 702 cultivar and 25 % in FH 696 cultivar as compared to control (83%). The results showed that no fungicide provided full disease inhibition, so, further investigation is needed to find the new chemistry against blight disease of sunflower crop.


Alternaria leaf blight; Fungicides; Helianthus annuus; Sunflower


Ali, Q., M. R. Yaseen and M. T. I. Khan. 2019. Energy budgeting and greenhouse gas emission in cucumber under tunnel farming in Punjab, Pakistan. Scientia Horticulturae, 250: 168-173.

Ando, K., K. M. Carr and R. Grumet. 2012. Transcriptome analyses of early cucumber fruit growth identifies distinct gene modules associated with phases of development. BMC Genomics, 13: 518. PMid:23031452 PMCid:PMC3477022

Cohen, Y. 1977. The combined effects of temperature, leaf wetness, and inoculum concentration on infection of cucumbers with Pseudoperonospora cubensis. Canadian Journal of Botany, 55: 1478-1487.

Cohen, Y., A. E. Rubin and M. Galperin. 2011. Formation and infectivity of oospores of Pseudoperonospora cubensis, the causal agent of downy mildew in cucurbits. Plant Disease, 95: 874-874. PMid:30731732

Colucci, S. J. 2008. Host range, fungicide resistance and management of Pseudoperonospora cubensis, causal agent of cucurbit downy mildew. (Unpublished) Master of Science thesis, North Carolina State University, Raleigh, North Carolina.

Delmas, C. E. L., Y. Dussert, L. Delière, C. Couture, I. D. Mazet, S. Richart Cervera and F. Delmotte. 2017. Soft selective sweeps in fungicide resistance evolution: recurrent mutations without fitness costs in grapevine downy mildew. Molecular Ecology, 26: 1936-1951. PMid:28063192

Ding, X., Y. Jiang, T. Hao, H. Jin, H. Zhang, L. He, Q. Zhou, D. Huang, D. Hui and J. Yu. 2016. Effects of heat shock on photosynthetic properties, antioxidant enzyme activity, and downy mildew of cucumber (Cucumis sativus L.). PLOS One, 11: e0152429. PMid:27065102 PMCid:PMC4827809

Dodds, P. N. and J. P. Rathjen. 2010. Plant immunity: towards an integrated view of plant-pathogen interactions. Nature Reviews Genetics, 11: 539-548. PMid:20585331

FAO. 2019. FAO Production Year Book. Food and Agriculture Organization of the United Nations, Rome, Italy.

Garrett, K. A., S. P. Dendy, E. E. Frank, M. N. Rouse and S. E. Travers. 2006. Climate Change Effects on Plant Disease: Genomes to Ecosystems. Annual Review of Phytopathology, 44: 489-509. PMid:16722808

Ghini, R., E. Hamada, R. R. V. Gonçalves, L. Gasparotto and J. C. R. Pereira. 2007. Análise de risco das mudanças climáticas globais sobre a sigatoka-negra da bananeira no Brasil. Fitopatologia Brasileira, 32: 197-204.

Gupta, S. K. and M. Gupta. 2018. Diseases of vegetables under protected cultivation conditions. Plant Disease Research, 33: 1-14.

Hijmans, R. J., G. A. Forbes and T. S. Walker. 2000. Estimating the global severity of potato late blight with GIS-linked disease forecast models. Plant Pathology, 49: 697-705.

Juroszek, P. and A. von Tiedemann. 2011. Potential strategies and future requirements for plant disease management under a changing climate. Plant Pathology, 60: 100-112.

Ketta, H. A., S. M. Kamel, A. M. Ismail and E. S. Ibrahiem. 2016. Control of downy mildew disease of cucumber using Bacillus chitinosporus. Egyptian Journal of Biological Pest Control, 26: 839-845.

Lebeda, A. and M. Widrlechner. 2003. A set of cucurbitaceae taxa for differentiation of Pseudoperonospora cubensis pathotypes. Journal of Plant Diseases Protection, 110: 337-349.

Legrève, A. and E. Duveiller. 2010. Preventing potential disease and pest epidemics under a changing climate. Climate change and crop production. CAB International, p. 50-70.

Palti, J. and Y. Cohen. 1980. Downy mildew of Cucurbits (Pseudoperonospora Cubensis): the Fungus and its hosts, distribution, epidemiology and control. Phytoparasitica, 8: 109-147.

Savory, E. A., L. L. Granke, L. M. Quesada-Ocampo, M. Varbanova, M. K. Hausbeck and B. Day. 2010. The cucurbit downy mildew pathogen Pseudoperonospora cubensis. Molecular Plant Pathology, 12: 217-226. PMid:21355994 PMCid:PMC6640371

Shetty, N. V., T. C. Wehner, C. E. Thomas, R. W. Doruchowski and K. P. Vasanth Shetty. 2002. Evidence for downy mildew races in cucumber tested in Asia, Europe, and North America. Scientia Horticulturae, 94: 231-239.

Srivastava, A., S. N. Kumar and P. K. Aggarwal. 2010. Assessment on vulnerability of sorghum to climate change in India. Agriculture, ecosystems environment, 138: 160-169.

Thomas, A. 2016. Biology, epidemiology and population genomics of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew. (Unpublished) thesis, North Carolina State University.

Urban, J. and A. Lebeda. 2006. Fungicide resistance in cucurbit downy mildew - methodological, biological and population aspects. Annals of Applied Biology, 149: 63-75.

VandenLangenberg, K. M. and T. C. Wehner. 2016. Downy Mildew Disease Progress in Resistant and Susceptible Cucumbers Tested in the Field at Different Growth Stages. HortScience, 51: 984-988.

Wolfe, D. W., L. Ziska, C. Petzoldt, A. Seaman, L. Chase and K. Hayhoe. 2007. Projected change in climate thresholds in the Northeastern U.S.: implications for crops, pests, livestock, and farmers. Mitigation and Adaptation Strategies for Global Change, 13: 555-575.

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DOI: 10.33687/phytopath.009.03.3450


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