Antagonistic Activity of Some Plant Growth Rhizobacteria to Fusarium Graminearum

Mohammed Abdulkareem, Hadi M. Aboud, Hutham M. Saood, Mohammed K. Shibly

Abstract


Biological control is an efficient and environmentally friendly way to prevent plant disease. In vitro technique was used to investigate the ability of Azospirillum sp., Azotobacter chroococcum, Pseudomonas flourscnes, Bacillus subtilis and Bacillus pumilus in order to control Fusarium graminearum. Results of dual culture interaction test shows that the colony diameter of Fusarium graminearum was significantly reduced (P <0.05) when combined individually with Azotobacter chroococcum, Bacillus pumilus and Bacillus subtilis. However, Azospirillum sp. and Pseudomonas flourscnes comparing to the control did not affect the growth of the pathogen. Volatile metabolites produced by Azospirillum sp. affects F. graminearum negatively (P < 0.05) at 3 and 6 days. The culture filtrate of Azospirillum sp. and Bacillus subtilis at three days measurement reduces the pathogen growth significantly. However, the effect of the former disappeared at 7 day while the latter continued affecting the growth of the pathogen significantly. Present study indicates acceptable levels of antagonistic activity of Azospirillum sp., Azotobacter chroococcum, Bacillus subtilis and Bacillus pumilus against Fusarium graminearum in vitro condition.

Keywords


Crown rot; antagonism; Azospirillum sp.; Azotobacter chroococcum; Pseudomonas flourscnes; Bacillus subtilis; Bacillus pumilus

References


Al-Mughrabi, K.I. 2010. Biological control of Fusarium dry rot and other potato tuber diseases using Pseudomonas fluorescens and Enterobacter cloacae. Biological Control. 53. 280-284.

Dal Bello, G.M., C.I. Mónaco, M.R. Simón. 2002. Biological control of seedling blight of wheat caused by Fusarium graminearum with beneficial rhizosphere microorganisms. World Journal of Microbiology and Biotechnology. 18. 627-636.

Davidson, J., M.H. Krysinska-Kaczmarek, Mckay A, E.S. Scott. 2012. Comparison of cultural growth and in planta quantification of Didymella pinodes, Phoma koolunga, and Phoma medicaginis var. pinodella, causal agents of ascochyta blight on field pea (Pisum sativum). . Mycologia. 104. 93-101.

Dwi, S., P. Siti, M. Erman. 2010. Control of Fusarium Wilt of Chili With Chitinolytic Bacteria. Hayati Journal of Biosciences. 17. 5-8.

Elshafie, H.S., I. Camele, E. Ventrella, L. Scrano, S. Lovelli, S.A. Bufo, M. Amato. 2013. Use of plant growth promoting bacteria (pgdb) for promoting tomato growth and its evaluation as biological control agent. International Journal of Microbiology Research. 5. 452-457.

Fekadu, A., A. Tesfaye. 2013. Antifungal activity of secondary metabolites of Pseudomonas fluorescens isolates as a biocontrol agent of chocolate spot disease (Botrytis fabae) of faba bean in Ethiopia. African Journal of Microbiology Research. 7. 5364-5373.

Francisco, D.H.C., M.B.P. Angelica, G.M. Gabriel, C.S. Melchor, R.H. Raul, N.A.G. Cristobal, C.R. Francisco. 2011. In Vitro antagonist action of Trichoderma Strains against Sclerotinia sclerotiorum and Sclerotium cepivorum. American Journal of Agricultural and Biological Science. 6. 410-417.

Harish, S., K. Manjula, A.R. Podile. 1998. Fusarium udum is resistant to the mycolytic activity of a biocontrol strain of Bacillus subtilis AF 1. FEMS Microbiology Ecology. 25. 385-390.

Huang, X., N. Zhang, X. Yong, X. Yang, Q. Shen. 2012. Biocontrol of Rhizoctonia solani damping-off disease in cucumber with Bacillus pumilus SQR-N43. Microbiological Research. 167. 135-143.

Ivana, P., V. Jelena, S. Mirjana, K. Dejana, R. Emil, S. Miloš, M.-M. Svetlana. 2012. Impact of Fungicides Used for Wheat Treatment on Button Mushroom Cultivation. Pesticidi i Fitomedicina. 27. 9-14.

María L. Tortora, M.L., V. Díaz-Ricci, R.O. Pedraza. 2011. Azospirillum brasilense siderophores with antifungal activity against Colletotrichum acutatum. Archives of Microbiology. 193. 275-286.

Murray, G., J. Brennan. 2009. Estimating disease losses to the Australian wheat industry. Australasian Plant Pathology. 38. 558–570

Obanora, F., S. Neateb, S. Simpfendorferc, R. Sabburga, P. Wilsond, S. Chakrabortya. 2013. Fusarium graminearum and Fusarium pseudograminearum caused the 2010 head blight epidemics in Australia. Plant Pathology. 62. 79–91.

Perveen K, N.A. Bokhari. 2012. Antagonistic activity of Trichoderma harzianum and Trichoderma viride isolated from soil of date palm field against Fusarium oxysporum. African Journal of Microbiology Research. 6. 3348-3353.

Saeideh, J.-P., H. Behzad, S. Akram. 2008. An in vitro study on the fungicidal effects of percidin 535® (per acetic acid 15%) against phytopathogenic fungi. Biotechnology. 7. 830-832.

Sansinenea, E., A. Ortiz. 2011. Secondary metabolites of soil Bacillus spp. Biotechnology Letters. 33. 1523-1538.

Sundaramoorthy, S., P. Balabaska. 2013. Evaluation of Combined Efficacy of Pseudomonas fluorescens and Bacillus subtilis in Managing Tomato Wilt Caused by Fusarium oxysporum f. sp. lycopersici (Fol). Plant Pathology Journal,. 12. 154-161.

Ting, A.S.Y., S.W. Mah, C.S. Tee. 2010. Identification of Volatile Metabolites from Fungal Endophytes with Biocontrol Potential towards Fusarium oxysporum F. sp. cubense Race 4. American Journal of Agricultural and Biological Science. 5. 177-182.

Yasar, E., E. Sezai, H. Ayhan, C. Ramazan. 2010. Effects of plant growth promoting rhizobacteria (PGPR) on rooting and root growth of kiwifruit (Actinidia deliciosa) stem cuttings. Biological Research. 43. 91-98.

Yuan, J., Y. Ruan, B. Wang, J. Zhang, R. Waseem, Q. Huang, Q. Shen. 2013. Plant Growth-Promoting Rhizobacteria Strain Bacillus amyloliquefaciens NJN-6-Enriched Bio-organic Fertilizer Suppressed Fusarium Wilt and Promoted the Growth of Banana Plants. Journal of Agricultural and Food Chemistry. 61. 3774-3780.

Zamanizadeh, H.R., N. Hatami, M.M. Aminaee, F. Rakhshandehroo. 2010. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. International Journal of Environmental Science and Technology. 8. 129-136.


Full Text: PDF

DOI: 10.33687/phytopath.003.01.0660

Refbacks

  • There are currently no refbacks.




Copyright (c) 2014 Mohammed Abdulkareem, Hadi M. Aboud, Hutham M. Saood, Mohammed K. Shibly

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.