Uncovering the Morphological and Genetical Heterogeneity of Pyricularia oryzae (cooke) sacc. in Southwestern Region of Bangladesh

Mst. Sabiha Sultana, Joyanti Ray, Papry Akther, Debproshad Mondal, Chhoa Mondal


Significant yield losses are reported due to blast disease caused by Pyricularia oryzae across all rice growing areas of the world. Though, management strategies reduce disease significantly but blast epidemics are still common. This research was aimed to determine the variability among the 19 Pyricularia oryzae isolates of South-Western regions of Bangladesh. Morphological variability was determined based on nine morphological components like front mycelial color, reverse mycelial color, growth behavior, conidial length (μm), conidial  width (μm), conidial color, radial growth at 12 days (mm), 14 days (mm) and 16 days (mm) after incubation. Cluster analysis considering nine morphological components showed five distinct groups. Front and reverse mycelial color, growth behavior and conidial length played more than 80% role in generating variation among the nine tested components. Higher genetic variation was also detected among the 19 isolates of P. oryzae.  Here, larger numbers of amplified DNA fragments was formed by Primers UCB-155 which showed distinct polymorphism. Between two clusters, Cluster II consists of 18 isolates that were originated from different origin but having maximum 94% genetical similarity. Cluster I contain only one isolates PO-16. From pair wise distance matrix, maximum distance (80%) was found between PO-16 and PO-03 isolates and minimum distance (6%) was obtained between the isolates PO-11 and PO-12. Results of this experiment revealed that, higher genetic diversity among isolates of P. oryzae existed in the south western region of Bangladesh but there has no direct relation with genetic diversity and geographical origin.


Pyricularia oryzae; morphological heterogeneity; genetical heterogeneity; RAPD; blast; Bangladesh


Aggarwal, R., V. B Singh., R. Shukla, M.S. Gurjar, S. Gupta and T.R. Sharma. 2010. URP-based DNA fingerprinting of Bipolaris sorokiniana isolates causing spot blotch of wheat. Journal Phytopathology. 158: 210–216. Available at: doi: 10.1111/j.1439-0434.2009.01603.x.

Aggarwal, R., V.B. Singh, M.S. Gurjar, S. Gupta and P. Srinivas. 2009. Intraspecific variations in Indian isolates of Bipolaris sorokiniana infecting wheat, based on morphological, pathogenic and molecular characters. Indian Phytopathology. 62(4): 449–460. Retrieved from https://epubs.icar.org.in/index.php/IPPJ/article/view/12636.

Bala, A. and Y. Vikal. 2015. Molecular characterization of Bipolaris sorokiniana populations from winter cereals. International Journal of Plant Protection, 8(2): 331–337. https://doi.org/10.15740/HAS/IJPP/8.2/331-337

Banerjee, S., R. Poswal, S. Gupta, S. Sharma, B. M. Bashyal and R. Aggarwal. 2014. Molecular characterization of Bipolaris spp. using universal rice primer (URP) markers. Indian Phytopathology. 67: 49-54.

Banglapedia. 2018. ‘National encyclopedia of Bangladesh 2018,’ Available at: http://en.banglapedia.org/index.php?title=Bengal_Delta

Barnett, H. L. and B. B. Hunter. 1972. Illustrated genera of imperfect fungi, (3rd ed)

Brown, S. D., and A. Lorenz. 2016. Single-step marker switching in Schizosaccharomyces pombe using a lithium acetate transformation protocol. Bio-protocol, 6(24): e2075. doi: 10.21769/BioProtoc.2075. PMID: 28352647; PMCID: PMC5366253.

Chuma, I., C. Isobe, Y.Hotta, K. Ibaragi, N. Futamata, M. Kusaba, K. Yoshida, R. Terauchi, Y. Fujita and H. Nakayashiki. 2011. Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species. PLoS Pathog. 7, e1002147.

Frazzon, A.P.G., A.T.S. Matsumura and S.T. van der Sand. 2002. Morphological characterization and genetic analysis of Drechslera teres isolates. Genetics and Molecular Biology, 25 (2): 235-241. https://doi.org/10.1590/S1415-47572002000200019

Gaddeyya G., P.S. Niharika, P. Bharathi and P.K.R. Kumar. 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. Adv. Appl. Sci. Res. 3:2020–2026. https://www.researchgate.net/publication/325550372_Isolation_and_identification_of_soil_mycoflora_in_different_crop_fields_at_Salur_Mandal_Advances_in_Applied_Science_Research_3_42020-2026_2012

Guo, L. D., K. D. Hyde and E. C. Liew. 2001. Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences. Molecular Phylogenetics and Evolution, 20(1): 1-13. https://doi.org/10.1006/mpev.2001.0942

Jabbarzadeh, Z., M. Khosh-Khui, H. Salehi and A. Saberivand. 2010. Inter simple sequence repeat (ISSR) markers as reproducible and specific tools for genetic diversity analysis of rose species. African Journal of Biotechnology. 9(37): 6091-6095. https://www.researchgate.net/publication/228477622_Inter_simple_sequence_repeat_ISSR_markers_as_reproducible_and_specific_tools_for_genetic_diversity_analysis_of_rose_species

Jaiswal, S. K., L. C. P. Sweta, S. Sharma, S. Kumar, R. Prasad, S. P. Pandy, R. Chand and A. K. Joshi. 2007. Identification of molecular marker and aggressiveness for different groups of Cochliobolus sativus isolates causing spot blotch disease in wheat (Triticum aestivum L.). Current Microbiology. 55 (2): 135-141. doi: 10.1007/s00284-007-0035-z. Epub 2007 Jul 20. PMID: 17647080.

Leisova-Svobodova, L., V. Minarikova, L. Kucera and S.A. Pereyra. 2011. Structure of the Cochliobolus sativus population variability. Plant Pathology. 61 (4): 709-718. https://doi.org/10.1111/j.1365-3059.2011.02547.x

Longya, A., S. Talumphai and C. Jantasuriyarat. 2020. Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers. Journal of fungi (Basel, Switzerland). 6(1): 38. https://doi.org/10.3390/jof6010038.

Mahto, B. N., S. Gurung., A. Nepal and T. B. Adhikari. 2012. Morphological, pathological and genetic variations among isolates of Cochliobolus sativus from Nepal. European Journal of Plant Pathology. 133: 405-417. https://doi.org/10.1007/s10658-011-9914-z

Mann, M. B., C. C. Spadari, T. Feltrin, A. P. G. Frazzon, J. C. Germani and S.T.V.D. Sand. 2014. Genetic variability of Bipolaris sorokiniana isolates using URP-PCR. Tropical Plant Pathology. 39(2): 163–171. Available at: https://doi.org/10.1590/S1982-56762014000200007

Moriyama, H., S.I. Urayama, T. Higashiura, T.M. Le and K. Komatsu. Chrysoviruses in Magnaporthe oryzae. Viruses. 2018;10:697. doi: 10.3390/v10120697.

Muller, D., C.M. Judd and V.Y. Yzerbyt. 2005. When moderation is mediated and mediation is moderated. J Pers Soc Psychol. 89(6): 852-63. doi: 10.1037/0022-3514.89.6.852. PMID: 16393020.

Natarajan, R. and S. A. Prathapar. 2013. Hydrogeology of the eastern Ganges Basin: an overview. International Water Management Institute (IWMI). https://dx.doi.org/10.5337/2013.216

Noguchi, M.T. 2011. Parasexual recombination in Magnaporthe oryzae. Jpn. Agric. Res. Q. 45: 39–45.

Powell, W., M. Morgante, C. Andre., M. Hanafey., J. Vogel., S. Tingey and Ragalski, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) marker for germplasm analysis. Molecular Breeding. 2: 225-238. https://doi.org/10.1007/BF00564200

Ram, T., N. D. Majumder., B. Mishra., M. M. Ansari and G. Padmavathi. 2007. Introgression of broad-spectrum blast resistance gene(s) into cultivated rice (Oryza sativa) from wild rice O. rufipogon. Current Science. 92(2): 25-30. https://www.jstor.org/stable/24096693

Rayhanul, M. I., Aminuzzaman, F. M., Chowdhury, M. S. M., Laila, L. and Ahmed, M. 2019. Survey on rice blast in some selected area of Bangladesh and in vitro evaluation of fungicides against Pyricularia oryzae. Bangladesh J. Plant Pathology, 35(1&2), 59-64. http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/3460

Rebecca, L. H., B. P. Zothansanga Singh, G. Gurusubramanian and N. K. Senthil. 2011. DNA finger printing of Bacillus thuringiensis based on prepetitive DNA sequences using ERIC-PCR. Sci Vis. 11(3): 147-154. https://www.sciencevision.org/storage/journal-articles/February2019/tqlZeT6W9mhzUk8uZQxA.pdf

Sanger, F., S. Nicklen and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 74(12): 5463-7. doi: 10.1073/pnas.74.12.5463.

Saitou, N. and M. Nei. 1987. The Neighbor-Joining Method A New Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution. 4(4): 406-425. https://doi.org/10.1093/oxfordjournals.molbev.a040454

Sharma, R. C., G. Ortiz-Ferrara., J. Crossa., M. R. Bhatta., M. A. Sufian., J. Shoran and R. Ortiz. 2007. Wheat grain yield and stability assessed through regional trials in the Eastern Gangetic Plains of South Asia. Euphytica, 157(3): 457–464. Available at: https://doi.org/10.1007/s10681-007-9470-y

Sneath, P.H.A. and R.R. Sokal. 1973. Numerical Taxonomy: The Principles and Practice of Numerical Classification. WF Freeman & Co., San Francisco, 573.

Sonah, H., R. K. Deshmukh., S. K. Parida., S. Chand and A. S. Kotasthane. 2009. Morphological and genetic variation among different isolates of Magnaporthe grisea collected from Chhattisgarh. Indian Phytopathology. 62 (4): 469–477. https://iranarze.ir/wp-content/uploads/2018/11/9388-English-IranArze.pdf

Suzuki H. 1969. Studies on the behaviour of the rice blast fungus spore and the application for forecasting method of the rice blast disease. Bull. 10:114–118. https://www.jircas.go.jp/sites/default/files/publication/jarq/08-2-078-083_0.pdf

Taheri P and A. Irannejad. 2014. Genetic structure of various Magnaporthe oryzae populations in Iran and Uruguay. Aus. Plant Pathol. 43 287-297. https://doi.org/10.1007/s13313-013-0269-0

Thompson, D. S., L. Patriccia and K. David. 2011. Co evolution of Morphology and Virulence in Candida Species. Eukaryotic cell. 10(9): 1173-82. doi: 10.1128/EC.05085-11

Utami, D. W., H. Aswidinnoor, S. Moeljopawiro, I. Hanarida and Reflinur. 2006. Inheritance of blast resistance (P. grisea Sacc) on interspecific crossing between IR 64 and Oryza rufipogon Sacc. HAYATI Journal of Biosciences, 13(3), 107. https://doi.org/10.4308/hjb.13.3.107

Yashaswini, Ch., B. Pushpavati and M.S. Madhav. 2017. Morphological and Molecular variability among rice blast pathogen (Magnaporthe oryzae) isolates in Southern India. Environment and Ecology. 35(4B):3015-3022. https://www.researchgate.net/publication/348565359_Morphological_and_Molecular_Variability_among_Rice_Blast_Pathogen_Magnaporthe_oryzae_Isolates_in_Southern_India

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DOI: 10.33687/phytopath.012.02.4686


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