Managing Citrus Canker: Evaluating the Effectiveness of Antibiotics and Chemicals for Disease Control
Citrus (family Rutaceae) stands as a prominent fruit crop on a global scale, bearing substantial significance. Renowned for its abundance of fiber, amino acids, antioxidants, vitamin C, and carbohydrates. However, the successful production of citrus is persistently challenged by the menacing presence of citrus canker caused by Xanthomonas citri subsp. citri (Xcc), posing 5-30% yield losses in Pakistan. Current study was aimed to manage Xcc through antibiotics and copper-based chemicals under lab. and field conditions. In vitro evaluations of antibiotics showed that Enrofloxacin expressed the maximum inhibition zone (35.68mm), followed by Enco-Mix (33.50mm), Pevivet-5 (33.48mm), Kanamycin sulphate (30.41mm), Sinobiotic (30.02mm), Streptomycin sulphate (29.33mm) and Gentam-20% (28.26mm) as compared to control. Field experiments of copper-based chemicals concluded that Copper nitrate exhibited the minimum disease incidence (16.07%), followed by Copper hydrooxide (21.78%), Amistar top (22.28%), Copper oxychloride (24.37%), and Copper acetate (24.63%), but Control expressed maximum disease incidence (65.00%). The mixture of Cu (NO2)3 + Enrofloxacin under field conditions was most effective regarding management of citrus canker. Current revelation appreciated the efficacy of Cu (NO2)3 + Enrofloxacin to manage citrus canker and strongly suggested this treatment against various bacterial pathogens.
Alexander, I. 2019. Exploitative beneficial effects of citrus fruits. In Citrus-Health Benefits and Production Technology. IntechOpen.
Ali, S., A. Hameed, G. Muhae-Ud-Din, M. Ikhlaq, M. Ashfaq, M. Atiq and Y. Wang. 2023. Citrus Canker: A Persistent Threat to the Worldwide Citrus Industry—An-Analysis. Agronomy, 13(4): 1112.
Al-Snafi, A.E. 2016. Nutritional value and pharmacological importance of citrus species grown in Iraq. IOSR Journal of Pharmacy, 6:76-108.
Anjum, T. and A. Javaid. 2005. Major diseases of citrus in Pakistan – a review. International Journal of Biology and Biotechnology, 2: 793-796.
Ashraf, S., G.A. Khan, S. Ali, M. Iftikhar and N. Mehmood. 2014. Managing insect pests & diseases of citrus: On farm analysis from Pakistan. Pakistan Journal of Phytopathology, 26: 301-307.
Bush, N.G., I. Diez-Santos, L.R. Abbott and A. Maxwell. 2020. Quinolones: mechanism, lethality and their contributions to antibiotic resistance. Molecules, 25: 5662.
Cook, A.A. 1988. Association of citrus canker pustules with leaf miner tunnels in North Yemen. Plant Disease, 72: 1-10.
FAO. 2017. Food and agriculture organization of united nations 2017, FAO 2017.
Graham, J.H. and R.P. Leite Jr. 2004. Lack of control of citrus canker by induced systemic resistance compounds. Plant Disease, 88: 745-750.
Hameed, A., M. Atiq, N.A. Rajput, H. Alsamadany, Y. Al-Zahrani, M.W. Alam and Z. Ahmed. 2022. In-vitro antibacterial potential of antibiotics against Xanthomonas axonopodis Pv. citri. Fresenius Environmental Bulletin, 31: 3886-3892.
Hameed, A., M. Atiq, Z. Ahmed, N.A. Rajput, M. Younas, A. Rehman, M.W. Alam, S. Sarfaraz, N. Liaqat, K. Fatima and K. Tariq. 2022. Predicting the impact of environmental factors on citrus canker through multiple regression. Plos one, 17: p.e0260746.
Jaskani, W. S. 2021. Citrus rootstock characterization against citrus canker and evaluation of antibiotics effect against Xanthomonas axonopodis pv. citri. Journal of Innovative Agriculture, 8: 1-8.
Kharat, V. M., S.B. Bramhankar, K.D. Thakur, S.S. Isokar, T. Pillai and G.T. Dinkwar. 2020. Management of citrus canker bacterium by using botanicals and chemicals. International Journal of Chemical Studies, 8: 1878-1882.
Li, Z.R., J. Li, W. Cai, J.Y. Lai, S.M. McKinnie, W.P. Zhang, B.S. Moore, W. Zhang and P.Y. Qian. 2019. Macrocyclic colibactin induces DNA double-strand breaks via copper-mediated oxidative cleavage. Nature chemistry, 11: 880-889.
Malik, A. R., S. Ali, M.N. Khan, S. Ahmad and M.A. Ali. 2020. Comparative efficacy of different antibiotics, fungicides and botanical extract for the control of citrus canker of kinnow mandarin. Pakistan Journal of Phytopathology, 32: 113-119.
Nethravathi, S.P. and K.B. Yadahalli. 2016. Evaluation of antibiotics and antibacterial chemicals against citrus canker disease. Indian Phytopathology, 69: 634-637.
Nirvan, R.S. 1961. Citrus canker and its control. Advances in Horticulture, 5: 171-175.
Rehman, M. A., S. Ali, M.B.S. Afzal, M.N. Khan and M. Ali. 2020. Efficacy of chemicals and botanical extracts to control citrus canker on Kinnow in Sargodha region. Journal of Innovative Sciences, 6: 101-107.
Sahi, S.T., M.U. Ghazanfar, M. Afzal, A. Rashed and A. Habib. 2007. Incidence of citrus canker disease caused by Xanthomonas campestris pv. citri (Hasse) dows on Kinnow (Citrus reticulata) and its chemotherapy. Pakistan Journal of Botany, 39: 1319.
Shams, S., W. Ahmad, A.H. Memon, S. Shams, Y. Wei, Q. Yuan and H. Liang. 2020. Cu/H 3 BTC MOF as a potential antibacterial therapeutic agent against Staphylococcus aureus and Escherichia coli. New Journal of Chemistry, 44:17671-17678.
Sharif, A., M.A. Khan, M. Atiq, N.A. Rajput, K. Fatima, E. Arif and H. Tariq. 2021. Prevalence, epidemiology and management of canker disease caused by Xanthomonas citri pv. citri in Sargodha district of Pakistan. Journal of Pure and Applied Algebra, 6: 1-08.
Sinha, M.K., R.C. Batra and D.K. Uppal. 1972. Role of citrus leaf-miner (Phyllocnistis citrella Staintan), on the prevalence and severity of citrus canker (Xanthomonas citri (Hasse) Dowson). Madras Agricultural Journal, 50: 240-245.
Sohi, G.S. and M.S. Sandhu. 1968. Relationship between citrus leafminer (Phyllocnistis citrella Stainton) injury and citrus canker (Xanthomonas citri (Hasse) Dowson) incidence on citrus leaves. Agricultural Research Journal- Punjab Agricultural University, 5: 66-69.
Stall, R.E., J.W. Miller, G.M. Marco and B.C. de Echenique 1980. Population dynamics of Xanthomonas citri causing cancrosis of citrus in Argentina. In Proceedings of the Florida State Horticultural Society, 93: 10-14.
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