Phenotyping Assays for Pathogenicity Determination of Fusarium oxysporum f. sp. cubense Tropical Race 4

Naimatullah Koondhar, Rehana N. Syed, Manzoor A. Abro, Abdul M. Lodhi, Muhammad N. Khan


Fusarium oxysporum f. sp. cubense (Foc) is a lethal pathogen of banana plant. Together with exclusion and eradication, exploration of resistance is of the utmost importance for its management. A simple and reproducible method of pathogenicity is required for the exploration of resistance in candidate genotypes. Therefore, the pathogenicity of Foc tropical race 4 (TR4) to tissue-cultured banana plants (cv., NIGAB-1, and Grand Naine) was done using three methods of inoculation, i.e., pouring method (PM), chlamydospore method (CM) and kernel method (KM). The latency period in both varieties inoculated by the chlamydospore method was two weeks after inoculation (wai), whereas, plants inoculated by pouring and kernel methods showed symptoms after three weeks of inoculation. The disease severity findings revealed a significant difference at the end of the assessment period with different method of inoculation. The pouring method of inoculation was found more effective than the other two methods since consistent, repeatable results were obtained by this method with dose-dependent final disease severity. It can be utilized for the evaluation of the pathogenicity of Foc (TR4) as well as to evaluate different disease control methods to find out effective control.


Panama disease; Banana; Phenotyping assays; Pathogenicity; Foc-TR4


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DOI: 10.33687/phytopath.013.01.4438


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