Phenotyping Assays for Pathogenicity Determination of Fusarium oxysporum f. sp. cubense Tropical Race 4

Naimatullah Koondhar, Rehana N. Syed, Manzoor A. Abro, Abdul M. Lodhi, Muhammad N. Khan

Abstract


Fusarium oxysporum f. sp. cubense (Foc) is a lethal pathogen of banana plant. Together with exclusion and eradication, exploration of resistance is of the utmost importance for its management. A simple and reproducible method of pathogenicity is required for the exploration of resistance in candidate genotypes. Therefore, the pathogenicity of Foc tropical race 4 (TR4) to tissue-cultured banana plants (cv., NIGAB-1, and Grand Naine) was done using three methods of inoculation, i.e., pouring method (PM), chlamydospore method (CM) and kernel method (KM). The latency period in both varieties inoculated by the chlamydospore method was two weeks after inoculation (wai), whereas, plants inoculated by pouring and kernel methods showed symptoms after three weeks of inoculation. The disease severity findings revealed a significant difference at the end of the assessment period with different method of inoculation. The pouring method of inoculation was found more effective than the other two methods since consistent, repeatable results were obtained by this method with dose-dependent final disease severity. It can be utilized for the evaluation of the pathogenicity of Foc (TR4) as well as to evaluate different disease control methods to find out effective control.

Keywords


Panama disease; Banana; Phenotyping assays; Pathogenicity; Foc-TR4

References


Ainsworth, G. C. 1971. Ainsworth and Bisby’s Dictionary of the Fungi. 6th ed. Commonwealth Mycological Institute, Kew, Surrey, England. 663 pp.

Ribeiro, L. R., E. P. Amorim, Z. J. M. Cordeiro, S. de Oliveira e silva, M. A. Dita. 2011. Discrimination of banana genotypes for Fusarium wilt resistance in the greenhouse, in Proceedings of the V International Symposium on Banana: ISHS-ProMusa Symposium on Global Perspectives on Asian Challenges, (Guangzhou: International Society for Horticultural Science), 897.

Bai, T., M. Qin, X. Li, H. Fan, S. Xu, L. Zeng, and S. J. Zheng. 2020. An additional threat to ‘Cavendish’banana growers and traders: The infection of banana peduncles by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4). Plant Health Progress, 21(4): 312-316.

Buddenhagen, I. 2009. Understanding strain diversity in Fusarium oxysporum f. sp. cubense and history of introduction of “Tropical Race 4” to better manage banana production. Acta Hortic. 828, 193–204.

Carvalhais, L. C., J. Henderson, V. A. Rincon-Florez, C. O’Dwyer, E. Czislowski, E. A. Aitken, and A. Drenth. 2019. Molecular diagnostics of banana Fusarium wilt targeting Secreted-in-Xylem genes. Frontiers in plant science, 547.

Dita, M. A., L. Teixeira, C. Li, S. Zheng, W. O'Neill, J. Daniels, L. Perez-Vicente, F. Carreel, V. Roussel, J. Carlier, C. Abadie, S. C. Carpentier, R. Iyyakutty, E. Kissel, J. van Wesemael, R. Chase, K. Tomekpe, N. Roux. 2021. Practical guidelines for early screening and field evaluation of banana against Fusarium wilt, Pseudocercospora leaf spots and drought. Montpellier (France): Bioversity International. 83p.

Dita, M. A., C. Waalwijk, I. W. Buddenhagen, M. T. Souza Jr, and G. H. J. Kema. 2010. A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen. Plant Pathology, 59(2): 348-357.

García-Bastidas, F. A., A. J. Van der Veen, G. Nakasato-Tagami, H. J. Meijer, R. E. Arango-Isaza, and G. H. J. Kema. 2019. An improved phenotyping protocol for Panama disease in banana. Frontiers in Plant Science, 10: 1006.

Khan, M. N., H. Lin, M. Li, J. Wang, Z. A. Mirani, S. I. Khan, M. A. Buzdar, I. Ali, and K. Jamil. 2017. Identification and growth optimization of a Marine Bacillus DK1-SA11 having potential of producing broad spectrum antimicrobial compounds. Pakistan Journal of Pharmaceutical Sciences, 30(3): 413-422.

Leslie, J. F. and B. A. Summerell. 2006. The Fusarium laboratory manual. Ames: Blackwell Publishing. https://doi.org/10.1002/9780470278376.

Lin, Y. H., J. Y. Chang, E. T. Liu, C. P. Chao, J. W. Huang, and P. F. L. Chang. 2009. Development of a molecular marker for specific detection of Fusarium oxysporum f. sp. cubense race 4. European Journal of Plant Pathology, 123(3): 353-365.

Manickkam, J., and M. J.P. 2023. Immunotoxicity of methanol extract of Entomopathogenic fungi, Aspergillus niger (OM514698) against House fly (Musca domestica) larva. https://europepmc.org/article/ppr/ppr593008.

Marquardt, S. 2001. “Green havoc”: Panama disease, environmental change, and labor process in the Central American banana industry. The American Historical Review, 106(1): 49-80.

Maymon, M., N. Sela, U. Shpatz, N. Galpaz, and S. Freeman. 2020. The origin and current situation of Fusarium oxysporum f. sp. cubense tropical race 4 in Israel and the Middle East. Scientific Reports, 10(1): 1-11.

Moore, N. Y., S. Bentley, K. G. Pegg, and D. R. Jones. 1995. Fusarium wilt of banana. Musa disease fact sheet.

Pande, S., J.N. Rao and M. Sharma, 2007. Establishment of the chickpea wilt pathogen Fusarium oxysporum f. sp. ciceris in the soil through seed transmission. Plant Pathology Journal, 23: 3-6.

Ploetz, R. C. 2006. Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology, 96(6): 653-656.

Ploetz, R. C. 2015. Management of Fusarium wilt of banana: A review with special reference to tropical race 4. Crop Protection, 73: 7-15.

Ploetz, R., and K. Pegg. 2000. Fusarium wilt. In Diseases of Banana, Abacá and Enset, ed D. Jones (Wallingford: CABI Publishing), 143–159.

Ribeiro, L. R., E. P. Amorim, Z. J. M. Cordeiro, S. de Oliveira e Silva, and M.A. Dita. 2011. Discrimination of banana genotypes for Fusarium wilt resistance in the greenhouse. In V International Symposium on Banana: ISHS-ProMusa Symposium on Global Perspectives on Asian Challenges 897 (pp. 381-385).

Siamak, S. B., and S. Zheng. 2018. Banana Fusarium wilt (Fusarium oxysporum f. sp. cubense) control and resistance, in the context of developing wilt-resistant bananas within sustainable production systems. Horticultural Plant Journal, 4(5): 208-218.

Smith, L. J., M. K. Smith, D. Tree, D. O’keefe, and V. J. Galea. 2008. Development of a small-plant bioassay to assess banana grown from tissue culture for consistent infection by Fusarium oxysporum f. sp. cubense. Australasian Plant Pathology, 37(2): 171-179.

Sugha, S.K., S.K. Kapoor and B.M. Singh, 1994. Factors influencing Fusarium wilt of chickpea (Cicer arietinum L.). Indian Journal of Mycology and Plant Pathology , 24: 97‒102.

Sun, E. J., and H. J. Su. 1984. Rapid method for determining differential pathogenicity of Fusarium oxysporum f. sp. cubense using banana plantlets. Tropical Agriculture (Trinidad and Tobago).

Thangavelu, R., D. Mostert, M. Gopi, P. G. Devi, B. Padmanaban, A. B. Molina, and A. Viljoen. 2019. First detection of Fusarium oxysporum f. sp. cubense tropical race 4 (TR4) on Cavendish banana in India. European Journal of Plant Pathology, 154(3): 777-786.

Thangavelu, R., M. Loganathan, R. Arthee, M. Prabakaran, and S. Uma. 2020. Fusarium wilt: a threat to banana cultivation and its management. CABI Reviews, (2020), 1-24.

Viljoen, A. 2002. The status of Fusarium wilt (Panama disease) of banana in South Africa. South African Journal of Science, 98(7): 341-344.

Viljoen, A., L. J. Ma, and A. B. Molina. 2020. Fusarium wilt (Panama disease) and monoculture banana production: Resurgence of a century-old disease. Emerging Plant Diseases and Global Food Security; Ristaino, JB, Records, A., Eds, 159-184.

Were, E., J. Schöne, A. Viljoen, and F. Rasche. 2022. Phenolics mediate suppression of Fusarium oxysporum f. sp. cubense TR4 by legume root exudates. Rhizosphere, 21, 100459.

Wu, Y. L., G. J. Yi, and X. X. Peng. 2010. Rapid screening of Musa species for resistance to Fusarium wilt in an in vitro bioassay. European Journal of Plant Pathology, 128(3): 409-415.

Zote, K.K., M.P. Haware, S. Jayanthi and J.N. Rao, 1996. Effects of inoculum density of Fusarium oxysporum f. sp. ciceris Race 1 and Race 2 on chickpea wilt. Phytopathologia Mediterranea, 35: 43-47.


Full Text: ACCEPTED

DOI: 10.33687/phytopath.013.01.4438

Refbacks

  • There are currently no refbacks.




Copyright (c) 2023 Dr Ghulam Hussain Jatoi

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.