Identification of Host Resistance against Bacterial Canker Disease on Apricot, Plum and Peach Grown in Punjab and KPK

Shagufta Bibi, Muhammad Inam-ul-Haq, Abid Riaz, Saad I. Malik


Bacterial canker disease caused by the pathogen Pseudomonas syringae pv. syrinagae (Pss) has been spread widely in Pakistan,  in Punjab and KPK provinces specially in strone fruits like apricot, Peach and Plum. During the 2014–2015 growing period, diseased samples were obtained from peach, plum and  apricot trees showing bacterial canker  symptoms  in Punjab and KPK provinces and 48 Pseudomonas syringae  isolates were obtained. The main aim of this research study was to find out host resistance in the cultivars of Apricot, Peach and Plum against bacterial canker caused by Pss in Peach, Plum and Apricot cultivars grown in Punjab and KPK province of Pakistan. In a pathogenicity test, three Pss isolates (PS3, PS9 and PS17) were found to be highly virulent in peach, plum and apricot  and were tested for cultivar resistance to Pss. Leaves and shoots of five varieties of Peach Early grand, Florida king, 4 ½, 5 ½, 6 ½, Four varieties of Plum Red beauty, Fazal-e- manani, Stanley, Producer and Two varieties of Apricot Chinese apricot and golden amber were foliar sprayed with a mixed culture of Pss having concentration of 10-8 cfu ml−1 . Sprayed cultivars were covered with plastic bags for 3 days for retaining moisture and kept in glass house and were keenly observed for appearance of  symptoms. 5 ½ of peach, Fazal-e-manani of plum and Chinese apricot of apricot were found resistant while 6 ½ of peach, Stanley of plum were found susceptible and Golden amber of apricot was found moderately susceptible to Pss. This is the first report of host resistance response of apricot, peach and plum cultivars grown in Pakistan to Pss.


Host Resistance; Bacterial Canker; Pseudomonas Syringae; Stone Fruits


Agrios, G. N. 2005. Plant Pathology. Academic Press: London.

Ahmed, R., M. Ashfaq, Z. Akram and M. Inam-ul-Haq. 2016. Pathogenesis and molecular confirmation of Pseudomonas syringae pv. syringae isolates from peach and plum in Pakistan. International Journal of Biosciences, 8: 22-28.

Ahmed, R., M. Inam-ul-Haq, U. Shahzad, S. Hyder, S. Shahzaman, A. Khan, H. Aatif, A. Ahmad and A. Gondal. 2018. First report of bacterial canker caused by Pseudomonas syringae pv. morsprunorum race 1 on peach from Khyber Pakhtunkhwa province of Pakistan. Plant Disease, 102: 2027-27.

Ali, N., A. Ali and M. A. Syed. 2021. Bacterial Plant Diseases and Their Management: Conventional Versus Modern Approaches. In, Microbial Biotechnology in Crop Protection. Springer.

Bassi, D. 1997. Apricot Culture: Present and Future. XI International Symposium on Apricot Culture 488.

Bibi, S., M. Inam-Ul-Haq, A. Riaz, S. I. Malik, M. I. Tahir and R. Ahmed. 2017. Screening and characterization of Rhizobacteria antagonistic to Pseudomonas syringae causing bacterial canker of stone fruits in Punjab and KPK. International Journal of Biosciences, 10: 405-12.

Crosse, J. 1959. Bacterial canker of stone-fruits .IV. Investigation of a method for measuring the inoculum potential of cherry trees. Annals of applied Biology, 47: 306-17.

Donmez, M. F., H. Karlidag and A. Esitken. 2010. Identification of resistance to bacterial canker (Pseudomonas syringae pv. syringae) disease on apricot genotypes grown in Turkey. European journal of plant pathology, 126: 241-47.

Doolotkeldieva, T. and S. Bobusheva. 2020. Characterization of Pseudomonas syringae pv. syringae from diseased stone fruits in Kyrgyzstan and testing of biological agents against pathogen. International Journal of Phytopathology, 9: 71-91.

Giovanardi, D., D. Dallai, L. Dondini, V. Mantovani and E. Stefani. 2016. Elicitation of resistance to bacterial canker of stone fruits by humic and fulvic acids (glucohumates): a cDNA-AFLP-dHPLC approach. Scientia Horticulturae, 212: 183-92.

Hetherington, S. 2005. Bacterial Canker of Stone Fruit. Research Horticulturist Health

Sciences, Science Alliance and Evaluation. Orange Agricultural Institute.

Ivanova, L. 2007. First occurrence of apricot blast disease caused by Psеudomonas syringae in the north-eastern part of Bulgaria. Balkan Symposium on Fruit Growing 825.

Johansson, O. N., A. K. Nilsson, M. B. Gustavsson, T. Backhaus, M. X. Andersson and M. Ellerström. 2015. A quick and robust method for quantification of the hypersensitive response in plants. PeerJ, 3: e1469.

Jones, A. M., S. E. Lindow and M. C. Wildermuth. 2007. Salicylic acid, yersiniabactin, and pyoverdin production by the model phytopathogen Pseudomonas syringae pv. tomato DC3000: synthesis, regulation, and impact on tomato and Arabidopsis host plants. Journal of Bacteriology, 189: 6773-86.

Kennelly, M. M., F. M. Cazorla, A. de Vicente, C. Ramos and G. W. Sundin. 2007. Pseudomonas syringae diseases of fruit trees: Progress toward understanding and control. Plant Disease, 91: 4-17.

King, E. O., M. K. Ward and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanin and fluorescin. The Journal of laboratory and clinical medicine, 44: 301-07.

Klement, Z., M. Sasser, K. Rudolph and D. Sands. 1990. Methods in Phytobacteriology. In, Identification of Bacteria Through Fatty Acid Analysis Budapest. Akademiai Kaido.

Kotan, R. and F. Şahin. 2002. First record of bacterial canker caused by Pseudomonas syringae pv. syringae, on apricot trees in Turkey. Plant pathology, 51: 798-98.

Lelliott, R. A. and D. E. Stead. 1987. Methods for the diagnosis of bacterial diseases of plants. Blackwell Scientific Publications.

Mohammadi, M., A. Ghasemi and H. Rahimian. 2001. Phenotypic characterization of Iranian strains of Pseudomonas syringae pv. syringae van Hall, the causal agent of bacterial canker disease of stone fruit trees. Journal of Agricultural Science and Technology, 3: 51-65.

Ong, K. and C. Rhodes. 2022. Gardening Bacterial Canker of Stone Fruits

Texas A & M AgriLife Extension, Texas A&M University. Texas, USA.

Taguchi, F., T. Suzuki, Y. Inagaki, K. Toyoda, T. Shiraishi and Y. Ichinose. 2010. The siderophore pyoverdine of Pseudomonas syringae pv. tabaci 6605 is an intrinsic virulence factor in host tobacco infection. Journal of Bacteriology, 192: 117-26.

Full Text: PDF

DOI: 10.33687/phytopath.011.01.4130


  • There are currently no refbacks.

Copyright (c) 2022 Muhammad Inam-ul-Haq

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.