1. Home
  2. Vol 11, No 1 (2022)
  3. Angèle

Qualities of Soybean Grains Harvested on Crops Treated with Neem Seeds Extract in Field Conditions against Asian Rust

Ndogho P. Angèle, Soupi N. M. Solange, Gbaporo G. F. Christian, Mvondo N. Dorotée, Ambang Zachée

Abstract


In soybean production, qualities of grains harvested are usually affected according to the development of diseases and control methods used in the field. This study aims to evaluate the impact of neem seeds extract (AENS) on soybean grains health after crops treatment in field conditions for the control against Asian rust. Experiment was conducted in Mimetala and Nkometou, located in the agroecological zone V of Cameroun. Three varieties of soybean (“Locale”, TGX 1835-10E and TGX1910-14E) were used in a completely randomized blocks design containing five treatments: T0 (control), T1, T2, T3 (25, 50, 100 g/L of extract respectively) and T4 (5.33 g/L of Plantineb 80wp). The disease was identified on the harvested grains; its incidence on those grains was evaluated and some biochemical parameters were assessed by extraction and determination of their contents. Many grains were attacked by Asian rust. The highest incidence was obtained in the seeds from untreated plots (35 %), compared to those from T1, T2, T3 (1.14; 1.07 and 0.95 % respectively). The different doses of AENS improved the content of all biochemical compounds tested in harvested grains compared with the T0 and T4 treatments. TGX1910-14E variety presented the highest content. In T3,grain contents in biochemical compounds were 415.17; 118.83; 118.33; 44.43; 36.53 and 10.83 mg/g of dry matter from total proteins, globulin, albumin, Glutelin, prolamin and glucide respectively. Meanwhile, in T4 treatments, grain contents were 403.49; 112.95; 112.78; 40.58; 33.90 and 9.91 mg/g of dry matter from the same compounds respectively. This study shows that the application of neem seeds extract on soybean crops for the control of Asian rust in field conditions at the maximum dose (e.g. 100 g/L) can improve the qualities of harvested grains. The neem extract as a natural substance should be used in integrated pest management of soybean and other crops.

Keywords


Asian rust; Biochemical profile; Glycine max; Aqueous extract; Neem seeds

References


Ambang, Z., N. Bekolo, E. Petga, J. Dooh and A. Asanga. 2007. Effect of crude extracts of Thevetia peruviana seeds on development of leaf spot disease of groundnut (Arachis hypogaea L.) caused by Cercospora sp. 8th African Crop Science Society Conference, El-Minia, Egypt.

Ambang, Z., B. Ndongo, G. Essono, J. Ngoh, P. Kosma, G. Chewachong and A. Asanga. 2011. Control of leaf spot disease caused by Cercospora sp. on groundnut (Arachis hypogaea) using methanolic extracts of yellow oleander (Thevetia peruviana) seeds. Australian Journal of Crop Science, 5: 227-32.

Awad, O. and A. Shimaila. 2003. Operational use of neem oil as an alternative anopheline larvicide. Part A: Laboratory and field efficacy. EMHJ-Eastern Mediterranean Health Journal, 9: 637-45. https://doi.org/10.26719/2003.9.4.637

Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72: 248-54. https://doi.org/10.1016/0003-2697(76)90527-3

Chumakov, A. E. and I. I. Zaharova. 1990. Influence and statistics of the development of diseases. In, Disease Damage to Agricultural Crops. Agrorome edition. Moscow.

Da-Silva, F. A. and S. S. Martinez. 2004. Effect of neem seed oil aqueous solutions on survival and development of the predator Cycloneda sanguinea (L.)(Coleoptera: Coccinellidae). Neotropical Entomology, 33: 751-57. https://doi.org/10.1590/S1519-566X2004000600013

Essoh Ngando, J., L. De Lapeyre de Bellaire and E. Fouré. 2006. La lutte chimique raisonnée contre la maladie des raies noires des bananiers au Cameroun: Evolution de la résistance aux fongicides. 8e conférence internationale sur les maladies des plantes industrielles, Tours.

Fromme, D. D., T. Price, J. Lofton, T. Isakeit, R. Schnell, S. Dodla, D. Stephenson, W. J. Grichar and K. Shannon. 2017. Effect of fungicide applications on grain sorghum (Sorghum bicolor L.) growth and yield. International Journal of Agronomy, 12: 1155-62. https://doi.org/10.1155/2017/5927816

Giller, e. D. 2007. Glycine max (L) Merill. In: H.A.M. Van der Vossen and G.S. Mkamilo (eds.), PROTA (Plant Resources of Tropical Africa). Wageningen, Pays Bas.

Goellner, K., M. Loehrer, C. Langenbach, U. Conrath, E. Koch and U. Schaffrath. 2010. Phakopsora pachyrhizi, the causal agent of Asian soybean rust. Molecular plant pathology, 11: 169-77. https://doi.org/10.1111/j.1364-3703.2009.00589.x

Javed, N., S. Gowen, M. Inam-ul-Haq and S. Anwar. 2007. Protective and curative effect of neem (Azadirachta indica) formulations on the development of root-knot nematode Meloidogyne javanica in roots of tomato plants. Crop protection, 26: 530-34. https://doi.org/10.1016/j.cropro.2006.05.003

Kosma, P., Z. Ambang, B. Begoude, G. M. Ten Hoopen, J. Kuaté and A. Akoa. 2011. Assessment of nematicidal properties and phytochemical screening of neem seed formulations using Radopholus similis, parasitic nematode of plantain in Cameroon. Crop protection, 30: 733-38. https://doi.org/10.1016/j.cropro.2011.02.026

Kumar, R. 2003. La lutte contre les insectes ravageurs et champignons pathogène. In, la situation de l'agriculture africaine. CTA, Kathala. Paris.

Labat, E. 2013. Le soja: Influence de sa consommation sur la santé humaine et conséquences de l'expansion de sa culture au niveau mondial, Université de Toulouse III Paul Sabastien.

Mboussi, S. B., Z. Ambang, A. Ndogho, J. Ngoh Dooh and F. Manga Essouma. 2016. In vitro antifungal potential of aqueous seeds extracts of Azadirachta indica and Thevetia peruviana against Phytophthora megakarya in Cameroon. Journal of Applied Life Sciences International, 4: 1-12. https://doi.org/10.9734/JALSI/2016/23710

Mweshi, M., Y. Deedat and F. S. Mwangala. 2010. Toxic effects of five plant extracts against the larger grain borer, Prostephanus truncatus. African Journal of Agricultural Research, 5: 3369-78.

Parween, T., S. Jan and T. Fatma. 2012. Evaluation of oxidative stress in Vigna radiata L. in response to chlorpyrifos. International Journal of Environmental Science and Technology, 9: 605-12. https://doi.org/10.1007/s13762-012-0095-x

Qiu, L.-J. and R.-Z. Chang. 2010. The origin and history of soybean. In: G Singh (ed.), The soybean: botany, production and uses.

Renard, F. and G. Foucart. 2008. Les maladies foliaires de la fin de végétation en culture de maïs. UCL-Louvain-la-Neuve. pp. 6.

Shahbaz, M., Z. Iqbal, A. Saleem and M. A. Anjum. 2009. Association of Lasiodiplodia theobromae with different decline disorders in mango (Mangifera indica L.). Pakistan Journal of Botany, 41: 359-68.

Sharma, V., S. Walia, J. Kumar, M. G. Nair and B. S. Parmar. 2003. An efficient method for the purification and characterization of nematicidal azadirachtins A, B, and H, using MPLC and ESIMS. Journal of Agricultural and Food Chemistry, 51: 3966-72. https://doi.org/10.1021/jf0342167

Siddiqui, Z. S. and S. Ahmed. 2006. Combined effects of pesticide on growth and nutritive composition of soybean plants. Pakistan Journal of Botany, 38: 721-33.

Voigt, J., B. Biehl and S. K. S. Wazir. 1993. The major seed proteins of Theobroma cacao L. Food Chemistry, 47: 145-51. https://doi.org/10.1016/0308-8146(93)90236-9

Wrather, A., G. Shannon, R. Balardin, L. Carregal, R. Escobar, G. Gupta, Z. Ma, W. Morel, D. Ploper and A. Tenuta. 2010. Effect of diseases on soybean yield in the top eight producing countries in 2006. Plant Health Progress, 23: 122-31. https://doi.org/10.1094/PHP-2010-0102-01-RS

Yemm, E. and A. Willis. 1954. The estimation of carbohydrates in plant extracts by anthrone. Biochemical journal, 57: 508-14. https://doi.org/10.1042/bj0570508

Zerrad, W., B. Maataoui, S. Hilali, S. El Antri and A. Hmyene. 2008. Etude comparative des mécanismes biochimiques de résistance au stress hydrique de deux variétés de blé dur. Lebanese Science Journal, 9: 27-36.


Full Text: PDF

DOI: 10.33687/phytopath.011.01.4044

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2022 Ambang Zachee

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.