Biocontrol Potentials of Plant Growth Promoting Rhizobacteria against Fusarium Wilt Disease of Cucurbit

Avinash T. Shanthi, Ravishankar R. Vittal


Fusarium spp., are the major soil-borne as well as seed borne pathogens causing wilt and rot diseases in more than 80 plant species including cucurbits. Fusarium spp., causes up to 100 % yield loss in the worldwide. Eleven isolates including three standard isolates were tested both in-vitro and in-vivo. In-vitro assay was done by dual culture method. Maximum inhibition was in case of Fusarium solani by Bacillus cereus MIC5. Sarratia spp. MIC1 antagonized the F. verticillodes and F. solani2. P. aeruginosa MIC2 inhibits all tested isolates F. oxysporum1. P. aeruginosa MTCC2581 suppressed the radial growth rate of F. oxysporum2. The two systemic fungicides used were chlorothalonil + mefenoxam (1000 ppm) and carbendazim (75 ppm to 500 ppm) which checked the growth of F. oxysporum. Carbendazim was more effective compared to mefenoxam + chlorothalonil at all tested concentrations. The crude extract of P. aeruginosa MIC2 developed in chloroform: methanol (9:1) showed a metabolite at Rf - 0.77 which it may be 2,4- diacetylphloroglucinol (DAPG), a broad-spectrum antimicrobial agent. Increased cucurbit seeds germination and seedling vigour was observed in B. amyloliquefaciens MIC6 (68% & 1576) and P. aeruginosa MTCC2581 (70% & 1929) in primed seeds. Further P. aeruginosa MTCC2581 can be tested in the field against the Fusarium wilt.


Fusarium spp.; PGPR; fungicides; biological control; DAPG


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DOI: 10.33687/phytopath.002.03.0351


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