Molecular Characterization of Penicillium expansum Isolated from Grapes and its Management by Leaf Extract of Chenopodium murale

Iqra Haider Khan, Arshad Javaid, Syeda Fakehha Naqvi

Abstract


Penicillium expansum Link causes an economically important postharvest blue mold disease in a number of fruits and vegetables. In the present study, this fungus was isolated from rotted grapes and identified on morphological basis. Identification of the pathogen was further confirmed on molecular basis by using four different primer pairs namely ITS, β-tubulin, CMD and CF under accession numbers MN752155, MN787831, MN787832 and MN787833, respectively. Leaf extract of Chenopodium murale was assessed for its potential to control in vitro growth of P. expansum. For this purpose, leaves were extracted in methanol and after evaporation of the solvent, the resulting extract was successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol followed by antifungal bioassays with different concentrations (1.562 to 200 mg mL-1) each organic solvent fraction. Although all the fractions variably controlled the fungal pathogen, however, n-butanol fraction showed the highest antifungal activity causing 45–86% reduction in biomass of the pathogen. Ethyl acetate fraction was also highly antifungal and reduced fungal biomass by 44–81%. Chloroform and n-hexane fractions were comparatively less effective and reduced biomass of P. expansum by 30–72% and 11–44%, respectively. This study concludes that ethyl acetate and n-butanol fractions are highly antifungal in nature against P. expansum.


Keywords


Penicillium expansum; Chenopodium murale; molecular identification; blue mold

References


Abdel-Wahhab, M., H. Ahmed, A. El-Nekeety, S. Abdel-Aziem, H. Shara, M. Abdelaziz, M. Sallam and F. Mannaa. 2020. Chenopodium murale essential oil alleviates the genotoxicity and oxidative stress of silver nanoparticles in the rat kidney. Egyptian Journal of Chemistry, 63: 2631-46.

Abdelhai, M. H., H. E. Tahir, Q. Zhang, Q. Yang, J. Ahima, X. Zhang and H. Zhang. 2019. Effects of the combination of baobab (Adansonia digitata L.) and Sporidiobolus pararoseus Y16 on blue mold of apples caused by Penicillium expansum. Biological Control, 134: 87-94. https://doi.org/10.1016/j.biocontrol.2019.04.009

Akhtar, R., A. Javaid and M. Z. Qureshi. 2020. Bioactive constituents of shoot extracts of Sisymbrium irio L. against Fusarium oxysporum f. sp. cepae. Planta Daninha, 38: e020200961. https://doi.org/10.1590/s0100-83582020380100008

Al-Batsh, L. J. and J. R. Qasem. 2020. Phytotoxicity of wild plants extracts to redroot pigweed (Amaranthus retroflexus L.) and nettle-leaved goosefoot (Chenopodium murale L.). Pakistan Journal of Agricultural Sciences, 57: 1441-56.

Amin, M. and J. Arshad. 2007. Exploitation of allelopathic potential of Chenopodium species to control charcoal rot pathogen of sunflower. Pakistan Journal of Agricultural Research, 20: 130-36.

Banaras, S., A. Javaid and I. H. Khan. 2020. Potential antifungal constituents of Sonchus oleraceous against Macrophomina phaseolina. International Journal of Agriculture and Biology, 24: 1376-82.

Belmaghraoui, W., A. Manni, M. Harir, A. Filali-Maltouf, O. K. E. Fatni and S. E. Hajjaji. 2018. Phenolic compounds quantification, antioxidant and antibacterial activities of different parts of Urtica dioica and Chenopodium murale. Research Journal of Pharmacy and Technology, 11: 5490. https://doi.org/10.5958/0974-360X.2018.01000.4

Chen, L. S., Y. J. Liu, S. W. Xu, Y. L. Chen, M. J. Chen and L. J. Zhou. 2017. First report of Penicillium polonicum causing blue mold on stored Polygonatum cyrtonema in China. Plant Disease, 101: 2149. https://doi.org/10.1094/PDIS-05-17-0756-PDN

Cheng, X., T. Ma, P. Wang, Y. Liang, J. Zhang, A. Zhang, Q. Chen, W. Li, Q. Ge, X. Sun and Y. Fang. 2020. Foliar nitrogen application from veraison to preharvest improved flavonoids, fatty acids and aliphatic volatiles composition in grapes and wines. Food Research International, 137: 109566. https://doi.org/10.1016/j.foodres.2020.109566 PMid:33233183

da Silva, L. E., C. Confortin and M. K. Swamy. 2020. Antibacterial and antifungal plant metabolites from the tropical medicinal plants. Advanced Structured Materials. Springer International Publishing. pp. 263-85. https://doi.org/10.1007/978-3-030-54027-2_7

Doyle, J. J. and J. L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 39-40.

Duduk, N., F. Bekčić, A. Žebeljan, N. Vučković and I. Vico. 2021. First report of blue mold caused by Penicillium crustosum on nectarine fruit in Serbia. Plant Disease, 105: 487. https://doi.org/10.1094/PDIS-07-20-1632-PDN PMid:32967562

FAOSTAT. 2020. FAO Production Year Book. Food and Agriculture Organization of the United Nations. Rome, Italy.

Farhan, M. S., A. H. Khamees, O. H. Ahmed, T. A. A. and Y. S. Yaseen. 2019. GC/MS analysis of n-hexane and chloroform extracts of Chenopodium murale leaves in Iraq. Journal of Pharmaceutical Research International, 31: 1-6. https://doi.org/10.9734/jpri/2019/v31i630325

Ghuffar, S., G. Irshad, F. Naz, H. B. Rosli, S. Hyder, N. Mehmood, M. A. Zeshan, M. M. Raza, C. G. Mayer and M. L. Gleason. 2018. First report of two Penicillium spp. causing postharvest fruit rot of grapes in Pakistan. Plant Disease, 102: 1037-37. https://doi.org/10.1094/PDIS-10-17-1616-PDN

González-Barreiro, C., R. Rial-Otero, B. Cancho-Grande and J. Simal-Gándara. 2014. Wine aroma compounds in grapes: A critical review. Critical Reviews in Food Science and Nutrition, 55: 202-18. https://doi.org/10.1080/10408398.2011.650336 PMid:24915400

He, C., Z. Zhang, B. Li, Y. Xu and S. Tian. 2019. Effect of natamycin on Botrytis cinerea and Penicillium expansum-Postharvest pathogens of grape berries and jujube fruit. Postharvest Biology and Technology, 151: 134-41. https://doi.org/10.1016/j.postharvbio.2019.02.009

Javaid, A., R. Munir, I. H. Khan and A. Shoaib. 2020. Control of the chickpea blight, Ascochyta rabiei, with the weed plant, Withania somnifera. Egyptian Journal of Biological Pest Control, 30: 1-8. https://doi.org/10.1186/s41938-020-00315-z

Jiao, W., X. Li, X. Wang, J. Cao and W. Jiang. 2018. Chlorogenic acid induces resistance against Penicillium expansum in peach fruit by activating the salicylic acid signaling pathway. Food Chemistry, 260: 274-82. https://doi.org/10.1016/j.foodchem.2018.04.010 PMid:29699670

Kassemeyer, H.-H. 2017. Fungi of Grapes. Biology of Microorganisms on Grapes, in Must and in Wine. Springer International Publishing. pp. 103-32. https://doi.org/10.1007/978-3-319-60021-5_4

Khan, I. H. and A. Javaid. 2020. Comparative antifungal potential of stem extracts of four quinoa varieties against Macrophomina phaseolina. International Journal of Agriculture and Biology, 24: 441-46.

Luciano‐Rosario, D., N. P. Keller and W. M. Jurick. 2020. Penicillium expansum: Biology, omics, and management tools for a global postharvest pathogen causing blue mould of pome fruit. Molecular Plant Pathology, 21: 1391-404. https://doi.org/10.1111/mpp.12990 PMid:32969130 PMCid:PMC7548999

Migicovsky, Z., J. Sawler, K. M. Gardner, M. K. Aradhya, B. H. Prins, H. R. Schwaninger, C. D. Bustamante, E. S. Buckler, G.-Y. Zhong, P. J. Brown and S. Myles. 2017. Patterns of genomic and phenomic diversity in wine and table grapes. Horticulture Research, 4: 1-11. https://doi.org/10.1038/hortres.2017.35 PMid:28791127 PMCid:PMC5539807

Morata, A., I. Loira, R. Vejarano, C. González, M. J. Callejo and J. A. Suárez-Lepe. 2017. Emerging preservation technologies in grapes for winemaking. Trends in Food Science & Technology, 67: 36-43. https://doi.org/10.1016/j.tifs.2017.06.014

Naqvi, S. F., A. Javaid and M. Z. Qureshi. 2019. Evaluation of antifungal potential of leaf extract of Chenopodium murale against Fusarium oxysporum f. sp. lycopersici. Planta Daninha, 37: e019199050. https://doi.org/10.1590/s0100-83582019370100139

Naqvi, S. F., I. H. Khan and A. Javaid. 2020. Hexane soluble bioactive components of Chenopodium murale stem. Pakistan Journal of Weed Science Research, 26: 425-32.

Papoutsis, K., M. M. Mathioudakis, J. H. Hasperué and V. Ziogas. 2019. Non-chemical treatments for preventing the postharvest fungal rotting of citrus caused by Penicillium digitatum (green mold) and Penicillium italicum (blue mold). Trends in Food Science and Technology, 86: 479-91. https://doi.org/10.1016/j.tifs.2019.02.053

Qasem, J. R. and H. A. Abu-Blan. 1995. Antifungal activity of aqueous extracts from some common weed species. Annals of Applied Biology, 127: 215-19. https://doi.org/10.1111/j.1744-7348.1995.tb06666.x

Samaras, Α., P. Ntasiou, C. Myresiotis and G. Karaoglanidis. 2020. Multidrug resistance of Penicillium expansum to fungicides: whole transcriptome analysis of MDR strains reveals overexpression of efflux transporter genes. International Journal of Food Microbiology, 335: 108896. https://doi.org/10.1016/j.ijfoodmicro.2020.108896 PMid:33070085

Tragni, V., P. Cotugno, A. De Grassi, F. Massari, F. Di Ronzo, A. M. Aresta, C. Zambonin, S. M. Sanzani, A. Ippolito and C. L. Pierri. 2021. Targeting mitochondrial metabolite transporters in Penicillium expansum for reducing patulin production. Plant Physiology and Biochemistry, 158: 158-81. https://doi.org/10.1016/j.plaphy.2020.07.027 PMid:33250320

Venkitasamy, C., L. Zhao, R. Zhang and Z. Pan. 2019. Grapes Integrated Processing Technologies for Food and Agricultural By-Products. Elsevier. pp. 133-63. https://doi.org/10.1016/B978-0-12-814138-0.00006-X

Vico, I., N. Duduk, M. Vasic and M. Nikolic. 2014. Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit. Pesticidi i fitomedicina, 29: 257-66. https://doi.org/10.2298/PIF1404257V

Wang, M., L. Zhao, X. Zhang, S. Dhanasekaran, M. H. Abdelhai, Q. Yang, Z. Jiang and H. Zhang. 2019. Study on biocontrol of postharvest decay of table grapes caused by Penicillium rubens and the possible resistance mechanisms by Yarrowia lipolytica. Biological Control, 130: 110-17. https://doi.org/10.1016/j.biocontrol.2018.11.004

Zhou, T., X. Wang, B. Ye, L. Shi, X. Bai and T. Lai. 2018. Effects of essential oil decanal on growth and transcriptome of the postharvest fungal pathogen Penicillium expansum. Postharvest Biology and Technology, 145: 203-12. https://doi.org/10.1016/j.postharvbio.2018.07.015


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DOI: 10.33687/phytopath.010.01.3506

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