Molecular Characterization of the Movement and Coat Proteins of a New Elm Mottle Virus Isolate Infecting European White Elm (Ulmus laevis Pall.)

Anne-Mareen Ellen Eisold


European white elms (Ulmus laevis Pall.) growing in a park in Caputh near Berlin (Germany) were regularly monitored over a period of 18 years showing virus infection-like symptoms such as chloroses, chlorotic ringspots, mottling and dieback. To obtain the evidence for viral infection, RNA-seq using an Illumina Hi Seq2500 was conducted and three contigs were obtained. They match with the three EMoV genomic RNAs and cover the open reading frames for the viral replicase, the polymerase and the movement and coat proteins (MP, CP). The contigs show identities of 95.3–96.4%, 91.9–93.3% and 89.0–92.5% at the nucleotide level with RNA 1, RNA 2 and RNA 3 of reference sequences, respectively. The analyses of the MP and CP showed significant differences in amino acid sequence compositions compared to those of reference EMoV sequences. These results demonstrate the presence of a so far unknown isolate of EMoV. This is the first report of sequence data of EMoV infecting U. laevis.


Illumina high throughput sequencing; EMoV; Ulmus laevis; RNA 3 variability; sequence analysis


Altschul, S. F., W. Gish, W. Miller, E. W. Myers and D. J. Lipman. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215: 403-10.

Ansel-McKinney, P., S. W. Scott, M. Swanson, X. Ge and L. Gehrke. 1996. A plant viral coat protein RNA binding consensus sequence contains a crucial arginine. The EMBO Journal, 15: 5077-84.

Aparicio, F. and V. Pallás. 2002. The molecular variability analysis of the RNA 3 of fifteen isolates of prunus necrotic ringspot virus sheds light on the minimal requirements for the synthesis of its subgenomic RNA. Virus Genes, 25: 75-84.

Aparicio, F., M. Vilar, E. Perez-Payá and V. Pallás. 2003. The coat protein of prunus necrotic ringspot virus specifically binds to and regulates the conformation of its genomic RNA. Virology, 313: 213-23.

Bandte, M., M. Essing, C. Obermeier and C. Büttner. 2004. Virus-diseased Ulmus laevis in eastern Germany. Forest Systems, 13: 65-69.

Bol, J. F. 2005. Replication of alfamo and ilar viruses: Role of the coat protein. Annual Review of Phytopathology, 43: 39-62.

Büttner, C., S. von Bargen, A.-M. Eisold, M. Bandte and M. Rott. 2015. Eine Fallstudie zum Elm mottle virus (EMoV) an Ulme (Ulmus sp.). In: D Dujesiefken (ed.), Jahrbuch der Baumpflege Braunschweig: Haymarket Media.

EPPO. 2017. PQR databaseEuropean and Mediterranean Plant Protection Organization. Paris, France.

Ge, X., S. W. Scott and M. T. Zimmerman. 1997. The complete sequence of the genomic RNAs of spinach latent virus. Archives of Virology, 142: 1213-26.

Herranz, M. C., J. A. Sánchez-Navarro, A. Saurí, I. Mingarro and V. Pallás. 2005. Mutational analysis of the RNA-binding domain of the Prunus necrotic ringspot virus (PNRSV) movement protein reveals its requirement for cell-to-cell movement. Virology, 339: 31-41.

Jaspars, E. M. J. 1998. A core promoter hairpin is essential for subgenomic RNA synthesis in alfalfa mosaic alfamovirus and is conserved in other Bromoviridae. Virus Genes, 17: 233-42.

Jones, A. T. and M. A. Mayo. 1973. Purification and properties of elm mottle virus. Annals of Applied Biology, 75: 347-57.

Kearse, M., R. Moir, A. Wilson, S. Stones-Havas, M. Cheung, S. Sturrock, S. Buxton, A. Cooper, S. Markowitz, C. Duran, T. Thierer, B. Ashton, P. Meintjes and A. Drummond. 2012. Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28: 1647-49.

King, A. M. Q., M. J. Adams, E. B. Carstens and E. J. Lefkowitz. 2012. Virus taxonomy: Classification and nomenclature of Viruses Ninth report of the International Committee on Taxonomy of Viruses. Academic Press Elsevier. San Diego, USA. pp. 486-87.

Larkin, M. A., G. Blackshields, N. P. Brown, R. Chenna, P. A. McGettigan, H. McWilliam, F. Valentin, I. M. Wallace, A. Wilm, R. Lopez, J. D. Thompson, T. J. Gibson and D. G. Higgins. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23: 2947-48.

MacFarlane, S. A. and W. J. McGavin. 2009. Genome activation by raspberry bushy dwarf virus coat protein. Journal of General Virology, 90: 747-53.

Marchler-Bauer, A., J. B. Anderson, F. Chitsaz, M. K. Derbyshire, C. DeWeese-Scott, J. H. Fong, L. Y. Geer, R. C. Geer, N. R. Gonzales and M. Gwadz. 2008. CDD: Specific functional annotation with the conserved domain database. Nucleic Acids Research, 37: 205-10.

Pallás, V., F. Aparicio, M. C. Herranz, K. Amari, M. A. Sánchez-Pina, A. Myrta and J. A. Sánchez-Navarro. 2012. Ilarviruses of Prunus spp.: A continued concern for fruit trees. Phytopathology, 102: 1108-20.

Pallás, V., F. Aparicio, M. C. Herranz, J. A. Sánchez-Navarro and S. W. Scott. 2013. The Molecular Biology of Ilarviruses Advances in Virus Research. Elsevier. pp. 139-81.

Pallás, V., J. A. Sánchez-Navarro and J. Díez. 1999. In vitro evidence for RNA binding properties of the coat protein of prunus necrotic ringspot ilarvirus and their comparison to related and unrelated viruses. Archives of Virology, 144: 797-803.

Pleše, N. and N. Juretić. 1999. Virus disease of field elm (Ulmus minor Mill.) in Croatia. Šumarski List, 123: 95-100.

Schmelzer, K. 1969. Das Ulmenscheckungs-Virus. Journal of Phytopathology, 64: 39-67.

Schmelzer, K. 1974. Investigations on viruses of ornamental and wild shrubs. 8th communication: New findings on Forsythia, Hydrangea and Philadelphus and viruses on Rhamnus, Centaurea, Galvezia, Cistus, Forestiera, Abeliophyllum, Celastrus, Staphylea and Crambe. ZBL Bact II, 129: 139-68.

Schmelzer, K., H. E. Schmidt and H. B. Schmidt. 1966. Viruskrankheiten und virusverdächtige Erscheinungen an Forstgehölzen. Archiv für Forstwesen, 15: 107-20.

Scott, S. W., M. T. Zimmerman and X. Ge. 2003. Viruses in subgroup 2 of the genus Ilarvirus share both serological relationships and characteristics at the molecular level. Archives of Virology, 148: 2063-75.

Shimura, H., C. Masuta, N. Yoshida, K. Sueda and M. Suzuki. 2013. The 2b protein of Asparagus virus 2 functions as an RNA silencing suppressor against systemic silencing to prove functional synteny with related cucumoviruses. Virology, 442: 180-88.

Sánchez-Navarro, J. A., J. F. Bol, C. B. Reusken and V. Pallás. 1997. Replication of alfalfa mosaic virus RNA 3 with movement and coat protein genes replaced by corresponding genes of Prunus necrotic ringspot ilarvirus. Journal of General Virology, 78: 3171-76.

Thomas, B. J., R. J. Barton and A. Tuszynski. 1983. Hydrangea mosaic virus, a new ilarvirus from Hydrangea macrophylla (Saxifragaceae). Annals of Applied Biology, 103: 261-70.

Untiveros, M., Z. Perez-Egusquiza and G. Clover. 2010. PCR assays for the detection of members of the genus Ilarvirus and family Bromoviridae. Journal of Virological Methods, 165: 97-104.

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DOI: 10.33687/phytopath.008.01.2742


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