Three Colletotrichum Species Responsible for Anthracnose on Synsepalum dulcificum (Miracle Fruit)

Hong H. Truong, Toyozo Sato, Seiju Ishikawa, Ayaka Minoshima, Takeaki Nishimura, Yuuri Hirooka


By 2016, fruit rot and two different leaf diseases (leaf spot and leaf blight) were found on Synsepalum dulcificum (miracle fruit) in Tokyo, Kanagawa and Kagoshima prefectures of Japan. From the lesions, abundant conidial masses and acervuli of three Colletotrichum species, two of which produced sexual state, were observed. We conducted a pathogenicity assay using these Colletotrichum species on healthy fruits and leaves of S. dulcificum. Our artificial inoculation tests showed symptoms of disease on tested fruit and leaf and indicated all three Colletotrichum species as causal agents of anthracnose on S. dulcificum. Based on morphological characters and molecular phylogenetic analyses using ITS, GAPDH, ACT, CAL and TUB2 loci, these species were identified as Colletotrichum aenigma (MAFF 246750), C. siamense (MAFF 246751) and C. karstii (MAFF 245966). They have been previously reported as plant pathogenic fungi elsewhere in the world. This is the first report of fruit rot, leaf blight and leaf spot on S. dulcificum caused by these three Colletotrichum species.


Colletotrichum aenigma; Colletotrichum karstii; Colletotrichum siamense; fruit rot; leaf anthracnose; miracle fruit; molecular phylogeny


Akinmoladun, A. C. 2016. Effect of Synsepalum dulcificum berry extract on oxidative stress and hepatotoxicity indices, following subacute administration in normal rats. FUTA Journal of Research in Sciences, 12: 167-177.

Alizadeh, A., M. Javan-Nikkhah, R. Zare, K. B. Fotouhifar, U. Damm and E. H. Stukenbrock. 2015. New records of Colletotrichum species for the mycobiota of Iran. Mycologia Iranica, 2: 95-109.

Cai, L., K. D. Hyde, P. W. J. Taylor, B. Weir, J. Waller, M. M. Abang, J. Z. Zhang, Y. L. Yang, S. Phoulivong and Z. Y. Liu. 2009. A polyphasic approach for studying Colletotrichum. Fungal Diversity, 39: 183-204.

Carbone, I. and L. M. Kohn. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia, 91: 553-556.

Chen, Y., G. Wei and W. Chen. 2002. New species of Pestalotiopsis. Mycosystema, 21: 316-323.

Da Silva, C. d. F. B. and S. J. Michereff. 2013. Biology of Colletotrichum spp. and epidemiology of the anthracnose in tropical fruit trees. Revista Caatinga, 26: 130-138.

Damm, U., P. F. Cannon, J. H. C. Woudenberg, P. R. Johnston, B. S. Weir, Y. P. Tan, R. G. Shivas and P. W. Crous. 2012. The Colletotrichum boninense species complex. Studies in Mycology, 73: 1-36.

De Silva, D. D., P. K. Ades, P. W. Crous and P. W. J. Taylor. 2016. Colletotrichum species associated with chili anthracnose in Australia. Plant Pathology, 66: 254-267.

Dean, R., J. A. L. Van Kan, Z. A. Pretorius, K. E. Hammond‐Kosack, A. Di Pietro, P. D. Spanu, J. J. Rudd, M. Dickman, R. Kahmann and J. Ellis. 2012. The top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology, 13: 414-430.

Diao, Y. Z., C. Zhang, F. Liu, W. Z. Wang, L. Liu, L. Cai and X. L. Liu. 2017. Colletotrichum species causing anthracnose disease of chili in China. Persoonia-Molecular Phylogeny and Evolution of Fungi, 38: 20-37.

Gan, P., N. Nakata, T. Suzuki and K. Shirasu. 2016. Markers to differentiate species of anthracnose fungi identify Colletotrichum fructicola as the predominant virulent species in strawberry plants in Chiba Prefecture of Japan. Journal of General Plant Pathology, 83: 14-22.

Gardes, M. and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology, 2: 113-118.

Guerber, J. C., B. Liu, J. C. Correll and P. R. Johnston. 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia, 95: 872-885.

Honger, J. O., S. K. Offei, K. A. Oduro, G. T. Odamtten and S. T. Nyaku. 2016. Identification and molecular characterisation of Colletotrichum species from avocado, citrus and pawpaw in Ghana. South African Journal of Plant and Soil, 33: 177-185.

Ichinose, R., H. Hoshi, T. Ono, Y. Hirooka and H. Horie. 2016. Anthracnose diseases found on Aesculus indica, Cornus kousa and Acer platanoides in Japan. Annual report of the kanto-tosan plant protection society. 63-123.

Lima, N. B., M. V. de A. Batista, M. A. De Morais, M. A. G. Barbosa, S. J. Michereff, K. D. Hyde and M. P. S. Câmara. 2013. Five Colletotrichum species are responsible for mango anthracnose in northeastern Brazil. Fungal Diversity, 61: 75-88.

Liu, F., M. Wang, U. Damm, P. W. Crous and L. Cai. 2016. Species boundaries in plant pathogenic fungi: A Colletotrichum case study. BMC Evolutionary Biology, 16: 81-86.

Maddison, W. P. 2017. Mesquite: A modular system for evolutionary analysis; 2007. Version 3.2.

Meetum, P., C. Leksomboon and M. Kanjanamaneesathian. 2015. First report of Colletotrichum aenigma and C. siamense, the causal agents of anthracnose disease of dragon fruit in Thailand. Journal of Plant Pathology, 97: 402.

Nguyen, P. T. H., O. Vinnere Pettersson, P. Olsson and E. Liljeroth. 2009. Identification of Colletotrichum species associated with anthracnose disease of coffee in Vietnam. European Journal of Plant Pathology, 127: 73-87.

O'Donnell, K. and E. Cigelnik. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution, 7: 103-116.

O’Donnell, K., H. I. Nirenberg, T. Aoki and E. Cigelnik. 2000. A multigene phylogeny of the Gibberella fujikuroi species complex: Detection of additional phylogenetically distinct species. Mycoscience, 41: 61-78.

Prihastuti, H., L. Cai, H. Chen, E. H. C. McKenzie and K. D. Hyde. 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity, 39: 89-109.

Rodrigues, J. F., R. d. S. Andrade, S. C. Bastos, S. B. Coelho and A. C. M. Pinheiro. 2016. Miracle fruit: An alternative sugar substitute in sour beverages. Appetite, 107: 645-653.

Rojas, E. I., S. A. Rehner, G. J. Samuels, S. A. Van Bael, E. A. Herre, P. Cannon, R. Chen, J. Pang, R. Wang, Y. Zhang, Y.-Q. Peng and T. Sha. 2010. Colletotrichum gloeosporioides s.l. associated with Theobroma cacao and other plants in Panamá: Multilocus phylogenies distinguish host-associated pathogens from asymptomatic endophytes. Mycologia, 102: 1318-1338.

Schena, L., S. Mosca, S. O. Cacciola, R. Faedda, S. M. Sanzani, G. E. Agosteo, V. Sergeeva and G. Magnano di San Lio. 2013. Species of the Colletotrichum gloeosporioides and C. boninense complexes associated with olive anthracnose. Plant Pathology, 63: 437-446.

Schoch, C. L., K. A. Seifert, S. Huhndorf, V. Robert, J. L. Spouge, C. A. Levesque, W. Chen, E. Bolchacova, K. Voigt and P. W. Crous. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences, 109: 6241-6246.

Sharma, G. and B. D. Shenoy. 2013. Colletotrichum fructicola and C. siamense are involved in chilli anthracnose in India. Archives of Phytopathology and Plant Protection, 47: 1179-1194.

Sharma, G. and B. D. Shenoy. 2016. Colletotrichum systematics: Past, present and prospects. Mycosphere, 7: 1093-1102.

Shi, Y.-C., K.-S. Lin, Y.-F. Jhai, B.-H. Lee, Y. Han, Z. Cui, W.-H. Hsu and S.-C. Wu. 2016. Miracle Fruit (Synsepalum dulcificum) exhibits as a novel anti-hyperuricaemia agent. Molecules, 21: 140-144.

Stamatakis, A., P. Hoover and J. Rougemont. 2008. A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology, 57: 758-771.

Wang, Y.-C., X.-Y. Hao, L. Wang, X. Bin, X.-C. Wang and Y.-J. Yang. 2016. Diverse Colletotrichum species cause anthracnose of tea plants (Camellia sinensis (L.) O. Kuntze) in China. Scientific Reports, 6: 35287.

Weir, B. S., P. R. Johnston and U. Damm. 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology, 73: 115-180.

Wikee, S., L. Cai, N. Pairin, E. H. C. McKenzie, Y.-Y. Su, E. Chukeatirote, H. N. Thi, A. H. Bahkali, M. A. Moslem, K. Abdelsalam and K. D. Hyde. 2010. Colletotrichum species from Jasmine (Jasminum sambac). Fungal Diversity, 46: 171-182.

Yang, Y., L. Cai, Z. Yu, Z. Liu and K. D. Hyde. 2011. Colletotrichum Species on Orchidaceaein Southwest China. Cryptogamie Mycologie, 32: 229-253.

Yokosawa, S., N. Eguchi, K.-i. Kondo and T. Sato. 2017. Phylogenetic relationship and fungicide sensitivity of members of the Colletotrichum gloeosporioides species complex from apple. Journal of General Plant Pathology, 83: 291-298.

Zivkovic, S., S. Stojanovic, Z. Ivanovic, N. Trkulja, N. Dolovac, G. Aleksic and J. Balaz. 2010. Morphological and molecular identification of Colletotrichum acutatum from tomato fruit. Pesticidi i Fitomedicina, 25: 231-239.

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DOI: 10.33687/phytopath.007.03.2658


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