Email this article 
Hide
Show all
Impact of Pre- and Post–Harvest Applications of Natural Antimicrobial Products on Apple and Pear Soft Rot Disease
Abstract
Three natural antibacterial compounds including bacteriocin like substance (BLS) produced from lactic acid bacteria (LAB), ethanolic extract of propolis (EEP), and nine plant extracts were evaluated against soft rot Bacillus strains. Testing in vivo these compounds were evaluated to control pear and apple soft rot disease. Among eight BLS tested, BLS of LAB2, LAB105 and LAB 107 exhibited the highest antibacterial activity as indicated by the formation of clear inhibition zone. Propolis extracts exhibited significant antibacterial activity against all tested soft rot Bacillus strains and it was noticed that the antibacterial activity was concentration dependent. Among nine plant extracts tested, extracts of Eucalyptus globulus and Psidium guajava exhibited the highest antibacterial activity. All tested antibacterial products significantly decreased apple and pear soft rot severity caused by Bacillus altitudinis compared to untreated control. The highest reduction percentage of soft rot severity was recorded for EEP followed by BLS from LAB and plant extracts tested, respectively. Combined pre-and post-harvest treatments of apple and pear with antimicrobial compounds proved to be more effective in reducing the soft rot severity and improved the physical and chemical properties of fruits during storage in both years of the study. The natural antimicrobial agents used in this study were promising compounds, since it seems to be more safe, economical and great potential for extending the shelf life and improve the quality of fruits. Therefore, the application of these compounds in the control of apple and pear soft rot could be advantageous for consumers, producers, and the environment.
Keywords
References
Adwan, G., B. Abu–Shanab, K. Adwan and F. Abu–Shanab. 2006. Antibacterial effects of nutraceutical plants growing in Palestine on Pseudomonas aeruginosa. Turk. J. Bio., 30: 239–242.
Agrios, G.N. 2007. Bacterial soft rots. In: Plant pathology Fifth edition, Academic press San Diego pages; 656.
Amadioha, A.C. 2000. Controlling rice blast in vitro and in vivo with extracts of Azadirachta indica. Crop Protec., 19: 287–290.
Antai, S.P. and M.H. Ibrahim. 1986. Microorganisms associated with African locust bean (Parkia filicoideaWelW). Fermentation for “dawadawa”production. J. Appl., Bacteriol., 61: 145–148.
Babayi, H., I. Kolo, J.I. Okogun and UJ Ijah. 2004. The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms. Biokemistri, 16: 106–111.
Bankova, V.S., S.L. de Castro and M.C. Marcucci. 2000. Propolis: recent advances in chemistry and plant origin. Apidologie, 31: 3–15.
Bartz, J., and A. Kelman. 1986. Reducing the potential for bacterial soft rot in potato tubers by chemical treatments and drying. Amer. J. Potato Res. 63: 481–93.
Basim, E., H. Basim and M. Zcan. 2006. Antibacterial activities of Turkish pollen and propolis extracts against plant bacterial pathogens. J. Food Eng. 77: 992–996.
Begum, S., S.I. Hassan, SN Ali and B.S. Siddiqui. 2004. Chemical constituents from the leaves of Psidium guajava. Nat. Prod. Res., 18: 135–140.
Bendaoud, H., J Bouajila, A. Rhouma, A. Savagnac and M. Romdhane. 2009. GC/MS analysis and antimicrobial and antioxidant activities of essential oil of Eucalyptus radiata. J. Sci. Food Agr., 89: 1292–1297.
Benvenuti, S., F. Pellati, M. Melegar and D. Bertelli. 2004. Polyphenols, anthocyanins, ascorbic acid and radical scavenging activity of Rubus, Ribes and Aronia. J. Food Sci., 69: 164–169.
Bergeron, C., A. Marston, E Hakizamungu and K. Hostettmann. 1995. Antifungal constituens of Chenopodium procerum. Intern .J. Pharmacognosy, 33: 15–119.
Brötz, H., M. Josten, I. Wiedemann, U. Schneider, F. Götz, G. Bierbaum and H.G. Sahl. 1998. Role of lipid–bound peptidoglycan precursors in the formation of pores by nisin, epidermin and other lantibiotics. Mol. Microbiol., 30: 317–327.
Burdock, G. A. 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem.Toxicol., 36: 347–363.
Cladera-Olivera, F., R. Geruza, A.S. Caron, A. A. Motta and A. Brandelli. 2006. Bacteriocin-like substance inhibits potato soft rot caused by Erwinia carotovora. Can. J. Microbiol. 52: 533–539.
Cock, I.E. 2008. Antibacterial activity of selected Australian native plant extracts. The Internet. J. Microbiol. 4(2). DOI: 10.5580/8d1.
Cowan, M.M. 1999. Plant product as antimicrobial agents. Clinical Microbiol Rev., 12: 564–582.
Dalmau, M., E. Maier, N. Mulet, M. Viñas and R. Benz. 2002. Bacterial membrane injuries induced by lactacin F and nisin. Int. Microbiol. 5: 73–80.
De Boer, H., A. Cuppels and A. Kelman. 1978. Pectolytic Erwinia spp. In the root zone of potato plants in relation to infestation of daughter plants. Phytopathology 64: 1784–1790.
De Vuyst, L. and E.J. Vandamme. 1994. Antimicrobial potential of lactic acid bacteria, p. 91-142.In:L.deVuyst and E. J. Vandamme (ed.), Bacteriocins of lactic acid bacteria: microbiology, genetics and applications. Blackie Academic & Professional, London, United Kingdom.
Derakhshan, S., M. Sattari and M. Bigdeli. 2008. Effect of sub inhibitory concentrations of cumin (Cuminum cyminum L.) seed essential oil and alcoholic extract on the morphology, capsule expression and urease activity of Klebsiella pneumoniae. Int. J. Antimicrob. Agents, 32: 43–2436.
Egharevba, H.O., I. Ibrahim, M.S. Abdullahi, S. Okwute, K. Okogun and I. Joseph. 2010. Broad spectrum antimicrobial activity of Psidium guajava Linn. leaf. Nat sci., 8: 43–50.
Elbanna, K., S. Elnaggar, A. Bakeer. 2014. Characterization of Bacillus altitudinis as a new causative agent of bacterial soft rot. J. Phytopathol, 162: 712–722.
Elbanna, K., G. Hassan, M.Khider and R.Mandour. 2010. Safe biodegradation of textile Azo Dyes by newly isolated lactic acid bacteria and detection of plasmids associated with degradation. J. Bioremed. Biodegrad, 1:11. doi:10.4172/2155–6199.1000112.
Focht, J., S.H. Hansen, J.V. Nielsen, S.A.Berg, D.Van and R. Riezler, 1993. Bactericidal Effect of propolis in vitro against agents causing upper respiratory tract infections. Arzneimitte Forschung, 43–II: 921–923.
Caili, F., S. Huan and L. Quanhong. 2006. A Review on pharmacological activities and utilization technologies of pumpkin. Plant Foods Hum Nut., 61:70–77.
Galvez, A., R.L. Lopez and H. Abriouel. 2008. Application of bacteriocins in the control of foodborne pathogenic and spoilage bacteria. Crit Rev. Biotechnol., 28: 125–152.
Gupta, V. K., A. Fatima, U. Faridi, A. S. Negi, K. Shanker, J. K. Kumar, N. Rahuja, S. Luqman, B. S. Sisodia, D. Saikia, M. P. Darokar, and S. P. Khanuja. 2008. Antimicrobial potential of Glycyrrhiza glabra roots. J. Ethno. Pharmacol. 116: 377–380.
Ivancajic, S., I. Mileusnic and C. Desanka. 2010. In vitro antibacterial activity of propolis extracts on 12 different bacteria in conditions of 3 various PH values. Arch.Biol. Sci., Belgrade, 62: 915–934.
Jack, R.W., J.R.Tagg and B.Ray. 1995. Bacteriocins of Gram–positive bacteria. Microbiological Reviews 59:171–200.
Kagale S., T. Marimuthu, B. Thayumanavan, R. Nandakumar and R. Samiyappan. 2004. Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiol. Mol Plant Pathol., 65: 91–100.
Katircio, H. and M.Nazime. 2006. Antimicrobial activity and chemical compositions of Turkish propolis from different regions. Afr J. Biotechnol., 5: 1151–1153.
Kayser, O. and H. Kolodziej. 1997. Antibacterial activity of extracts and constituents of Pelargonium sidoides and Pelargonium reniforme. Plantamedica, 63 (6):508–510.
Klaenhammer, T.R.. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev., 12: 39–86.
Kosalec, I., M.Bakmaz, S. Pepeejnjak and S.V. Kneževi. 2004. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharmaceut, 54: 65–72.
Krebs, H., B. Dorn and H.R. Forrer. 2006. Control of late blight of potato with medicinal plant suspensions. Agrarforschung, 13: 16–21.
Krell, R. 1996. Value-Added Products from Beekeeping. FAO Agricultural Services Bulletin, No: 124, Food and Agriculture Organization of the United Nation, Rome, pp. 85–89.
Kremer, F. W. and G. Unterstenhofer. 1967. Evaluation of results phytosanitary according to the method of Townsend and Heuberger. Institute of Biology Farbenfabriken, of Bayer A. G., Leverkusen, 20:657-660.
Latha, P., T. and, N. Ragupathi, V. Prakasam and R. Samiyappan. 2009. Antimicrobial activity of plant extracts and induction of systemic resistance in tomato plants by mixtures of PGPR strains and Zimmu leaf extract against Alternaria solani. Bio Control, 50: 85–93.
Lavermicocca, P., F. Valerio, A. Evidente, S. Lazzaroni, A. Corsetti and M. Gobbetti. 2000. Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus plantarum strain 21B. Appl. Environ. Microbiol., 66: 4084–4090.
Lima, G., F. De Curtis, R. Castoria, S. Pacifica and V. DeCicco. 1998. Additives and natural products against post-harvest pathogens compatibility with antagonistic yeasts. In: Plant Pathology and Sustainable Agriculture. Proceeding of the Sixth SIPaV Annual Meeting, Campobasso, 17–18 September.
Lund, B.M.. 1983. Bacterial spoilage, pp. 219–257. In: Demis C (Ed.). Post-harvest Pathology of Fruits and Vegetables, Academic Press, London.
Meyer, A.S., K.I Suhr and P.Nielsen. 2002. Natural Food Preservatives. pp: 130–131. In: Minimal Processing Technologies in the Food Industry, Ohlsson, T. and N. Bengtsson (Eds.). Wood head Publishing Limited and CRC Press LLC., Cambridge, Boca Raton.
Montville, T.J., K.Winkowski and R. D. Ludescher. 1995. Models and mechanisms for bacteriocin action and application. Inter. Dairy J., 5: 797–814.
Motta, A.S., and A. Brandelli, 2002. Characterization of an antibacterial peptide produced by Brevibacterium linens. J. Appl. Microbiol., 92: 63–70.
Narayanasamy, P. 2006. Postharvest Pathogens and Disease Management. A John Wiley & Sons, Inc., Hoboken, NJ, New Jersey, USA pp. 563.
O`Sullivan, L., R.P. Ross and C. Hill. 2002. Potential of bacteriocin producing Lactic acid bacteria for improvements in food safety and quality. Biochimie, 84: 593–604.
Ordoñez, R.M., I.C.Zampini, N.M.Moreno and M.I. Isla. 2011. Potential application of Northern Argentine propolis to control some phytopathogenic bacteria. Microbiol Res., 166: 578–584.
Pocharski, W.J., D. Konopacka and Z. J.wierz. 2000. Comparison of Magness_Taylor pressure test with mechanical, nondestructive methods of apple and pear firmness measurements. Int Agrophysics, 14: 311–31.
Quattrucci, A., E. Ovidi, A. Tiezzi, V. Vinciguerra and G.M. Balestra. 2013. Biological control of tomato bacterial speck using Punica granatum fruit peel extract. Crop Protec., 46: 18–22.
Rosalia, T., B. Lluís, M. Emilio and B. Esther. 2008. Lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi. Inter Microbiol., 11: 231–236.
Schnürer, J. and M J. Agnusson. 2005. Antifungal lactic acid bacteria as biopreservatives. Trends Food Sci. Technol, 16: 70 –78.
Settanni, L., O. Massitti, D. Van Sinderen and A. Corsetti. 2005. In situ activity of a bacteriocin-producing Lactococcus lactis strain. influence on the interactions between lactic acid bacteria during sourdough fermentation. J. Appl. Microbiol., 99: 670–681.
Settanni, L. and A. Corsetti. 2008. Application of bacteriocins in vegetable food biopreservation. Inter. J. Food Microbiol., 121: 123–138.
Shruthi, S. D., A. Roshan, S.S. Timilsina, and S. Sunita. 2013. A Review on the medinical plant Psidium guajava. J. Drug Deliv. and Therap., 3: 1621–68.
Singleton, V. and J. Rossi. 1965. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Amer. J. Enol.Vitic., 6:144–158.
Steel, R.D., J. H. Torrie and D.A. Dickey. 1997. Principles and Procedures of Statistics: A Biometrical Approach. 3rd edition, McGraw–Hill Inc., New York, USA, 612 pp.
Trusheva, S. B., M. Popova , V. Bankova, S. Simova , M.C. Marcucci, P. Miorin, P. Laguna, R. Flavia and I. Tsvetkova. 2006. Bioactive Constituents of Brazilian Red Propolis. doi:10.1093/ecam/nel006 Evidence–based Complementary and Alternative Medicine 3: 249–254.
Wolf, C. E. and W. R. Gibbons. 1996. Improved method for quantification of the bacteriocin nisin. J. Appl. Bacteriol., 80: 453-457.
Zhang, H.Y., X.D. Zheng, C. X. Fu and Y. F. Xi. 2005. Postharvest biological control of gray mold rot of pear with Cryptococcus laurentii. Postharvest Biol.Technol., 35: 79–86.
DOI: 10.33687/phytopath.004.03.1329
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Abdelradi T. Bakeer, Khaled Elbanna, Sameh A. Elnaggar
This work is licensed under a Creative Commons Attribution 4.0 International License.
