Efficacy and Cost-Benefit Analysis of Fungicide Combinations against Soybean Rust and Cercospora Leaf Blight in Paraguay

Marcos Javier Mendoza-Duarte, Milciades Ariel Melgarejo-Arrua, Andrea Celeste Zarza-Cabrera, Nery Rubén Villasboa-Cardozo, Marta Alicia Fernandez-Gamarra, Marco Maidana-Ojeda, Andrea Alejandra Arrua, Guillermo Andrés Enciso-Maldonado

Abstract


This study evaluated the efficacy, seed yield and economic returns of nine fungicide mixtures for the integrated management of Asian soybean rust (Phakopsora pachyrhizi) and Cercospora leaf blight (Cercospora kikuchii) under field conditions in Canindeyú, Paraguay, during the 2023/24 and 2024/25 seasons. Trials were arranged in a randomized complete‐block design with four replicates, comparing premixes of QoI, DMI, SDHI and multisite actives. Disease severity was monitored over time to calculate the area under the disease progress curve (AUDPC) and control efficacy. Grain yield, thousand‐seed weight, gross return, treatment cost and benefit–cost ratio (B/C) were also determined. In both seasons, mixtures combining systemic actives (QoI + DMI ± SDHI) achieved the greatest disease suppression, reducing AUDPC values by > 80 % relative to the untreated control and exceeding 95 % control efficacy under higher disease pressure. These treatments outyielded the control by 700–1 000 kg ha⁻¹ and increased thousand‐seed weight by up to 4 g. Although multisite fungicides (mancozeb, chlorothalonil) delivered moderate disease control (50–70 %), their low cost yielded the highest B/C ratios (> 16). Overall net benefits ranged from USD 770 to USD 1 150 ha⁻¹. We conclude that locally validated systemic–multisite fungicide mixtures provide robust, economically viable control of late-season soybean foliar diseases in Paraguay and support sustainable disease management under variable epidemic conditions.


Keywords


Phakopsora pachyrhizi; Cercospora spp.; chemical control; disease progress curves; FRAC resistance management; benefit-cost ratio

References


Alves, K. S., Barro, J. P., Guimarães, M., & Del Ponte, E. M. (2021). Profitability of fungicide applications for managing soybean rust in scenarios of variable efficacy and costs: a stochastic simulation. Plant Pathology, 70(6), 1354-1363. https://doi.org/10.1111/ppa.13396

Barro, J. P., Alves, K. S., Godoy, C. V., Dias, A. R., Forcelini, C. A., Utiamada, C. M., ... & Del Ponte, E. M. (2021). Performance of dual and triple fungicide premixes for managing soybean rust across years and regions in Brazil: A meta‐analysis. Plant Pathology, 70(8), 1920-1935. https://doi.org/10.1111/ppa.13418

Barro, J. P. (2022). Epidemiology and management of frogeye leaf spot of soybean: Damage thresholds, efficacy and profitability of foliar fungicides (Doctoral dissertation, Universidade Federal de Viçosa). Locus UFV. https://locus.ufv.br/handle/123456789/30724

Caballero-Mairesse, G. G., Mendes, F. H., Arrua, A. A., Lopez-Nicora, H. D., & Enciso-Maldonado, G. A. (2024). Perception of farmers on the management of fungal diseases of soybeans (Glycine max (L.) Merr.) in Paraguay. Agronomía Mesoamericana, 35(1). http://dx.doi.org/10.15517/am.2024.55193

Dalla Lana, F., Ziegelmann, P. K., Maia, A. D. H., Godoy, C. V., & Del Ponte, E. M. (2015). Meta-analysis of the relationship between crop yield and soybean rust severity. Phytopathology, 105, 307–315. https://doi.org/10.1094/PHYTO-06-14-0157-R

Edwards Molina, J. P., Paul, P. A., Amorim, L., da Silva, L. H. C. P., Siqueri, F. V., Borges, E. P., ... & Godoy, C. V. (2019). Meta‐analysis of fungicide efficacy on soybean target spot and cost–benefit assessment. Plant Pathology, 68(1), 94-106. https://doi.org/10.1111/ppa.12925

Fernandes, A. F. D., Stilgenbauer, S., Brahm, L., Stammler, G., & von Tiedemann, A. (2025). Identification of Cercospora species associated with Cercospora Leaf Blight of soybean in Brazil. Journal of Plant Diseases and Protection, 132(1), 1-14. https://doi.org/10.1007/s41348-024-01008-7

Fungicide Resistance Action Committee. (2024). FRAC Code List 2024: Fungal control agents sorted by cross-resistance pattern and mode of action (including coding for FRAC groups on product labels) [PDF]. https://www.frac.info/media/kufnaceb/frac-code-list-2024.pdf

Godoy, C. V., Koga, L. J., & Canteri, M. G. (2006). Diagrammatic scale for assessment of soybean rust severity. Fitopatologia Brasileira, 31, 63-68. https://doi.org/10.1590/S0100-41582006000100011

Hartman, G. L., Rupe, J. C., Sikora, E. F., Domier, L. L., Davis, J. A., & Steffey, K. L. (2015). Compendium of soybean diseases and pests 5th ed. St. Paul.

Hossain, M. M., Sultana, F., Yesmin, L., Rubayet, M. T., Abdullah, H. M., Siddique, S. S., ... & Yamanaka, N. (2024). Understanding Phakopsora pachyrhizi in soybean: comprehensive insights, threats, and interventions from the Asian perspective. Frontiers in Microbiology, 14, 1304205. https://doi.org/10.3389/fmicb.2023.1304205

Langenbach, C., Campe, R., Beyer, S. F., Mueller, A. N., & Conrath, U. (2016). Fighting Asian soybean rust. Frontiers in plant science, 7, 797. https://doi.org/10.3389/fpls.2016.00797

Machado, F. J., Barro, J. P., Godoy, C. V., Dias, A. R., Forcelini, C. A., Utiamada, C. M., ... & Del Ponte, E. (2022). Is tank mixing site-specific premixes and multi-site fungicides effective and economic for managing soybean rust? A meta-analysis. Crop Protection, 151, 105839. https://doi.org/10.1016/j.cropro.2021.105839

Martins, M. C., Guerzoni, R. A., Câmara, G. M. D. S., Mattiazzi, P., Lourenço, S. A., & Amorim, L. (2004). Escala diagramática para a quantificação do complexo de doenças foliares de final de ciclo em soja. Fitopatologia Brasileira, 29, 179-184. https://doi.org/10.1590/S0100-41582004000200009

Machado, F. J., Barro, J. P., Godoy, C. V., Dias, A. R., Forcelini, C. A., Utiamada, C. M., ... & Del Ponte, E. (2022). Is tank mixing site-specific premixes and multi-site fungicides effective and economic for managing soybean rust? A meta-analysis. Crop Protection, 151, 105839. https://doi.org/10.1016/j.cropro.2021.105839

Meira, D., Woyann, L. G., Bozi, A. H., Milioli, A. S., Beche, E., Panho, M. C., ... & Benin, G. (2020). Asian soybean rust: a scientometric approach of Phakopsora pachyrhizi studies. Euphytica, 216, 1-12. https://doi.org/10.1007/s10681-020-02667-x

Müller, M. A., Stammler, G., & De Mio, L. L. M. (2021). Multiple resistance to DMI, QoI and SDHI fungicides in field isolates of Phakopsora pachyrhizi. Crop Protection, 145, 105618. https://doi.org/10.1016/j.cropro.2021.105618

Pagano, M. C., & Miransari, M. (2016). The importance of soybean production worldwide. In Abiotic and biotic stresses in soybean production (pp. 1-26). Academic Press. https://doi.org/10.1016/B978-0-12-801536-0.00001-3

R Core Team. 2025. R: A language and environment for statistical computing (Version 4.3.0) [Software]. R Foundation for Statistical Computing. https://www.R-project.org/

Reis, E. M., Zanatta, M., Pereira da Silva, L. H. C., & Reis, A. C. (2022). Asian soybean rust control efficacy calculated with AUDPC and with final severity data. Summa Phytopathologica, 48(1), 28-31. https://doi.org/10.1590/0100-5405/187471

Sautua, F. J., Pizá, M. C. P., Scandiani, M. M., & Carmona, M. A. (2024). Cercospora leaf blight and purple seed stain of soybean: A permanent challenge. Plant Pathology, 73(8), 1981-2004. https://doi.org/10.1111/ppa.13956

Scherm, H., Christiano, R. S. C., Esker, P. D., Del Ponte, E. M., & Godoy, C. V. (2009). Quantitative review of fungicide efficacy trials for managing soybean rust in Brazil. Crop Protection, 28(9), 774–782. https://doi.org/10.1016/j.cropro.2009.05.006

Scholz, R., Fernández Gamarra, M. A., Vargas, M. J., & Yamanaka, N. (2024). Yearly changes in virulence of Phakopsora pachyrhizi isolates in Paraguay. Tropical Plant Pathology, 49(3), 413-420. https://doi.org/10.1007/s40858-024-00639-3

Shaner, G., & Finney, R. E. (1977). The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology, 67(8), 1051-1056.

Shrestha, B. K., Ward, B. M., Allen, T. W., da Silva, E. T., Zulli, H., Dunford, W., Doyle, V., Bradley, C. A., Buckley, B., Chen, P., Clubb, M., Kelly, H., Koebernick, J., Padgett, B., Rupe, J. C., Sikora, E. J., Spurlock, T. N., Thomas-Sharma, S., Tolbert, A., … Price, P. P., III. (2024). Characterization of QoI-fungicide resistance in Cercospora isolates associated with Cercospora leaf blight of soybean from the southern United States. Plant Disease, 108(1), 149–161. https://doi.org/10.1094/PDIS-03-23-0588-RE

Yorinori, J. T., Paiva, W. M., Frederick, R. D., Costamilan, L. M., Bertagnolli, P. F., Hartman, G. E., ... & Nunes Jr, J. (2005). Epidemics of soybean rust (Phakopsora pachyrhizi) in Brazil and Paraguay from 2001 to 2003. Plant Disease, 89(6), 675-677. https://doi.org/10.1094/PD-89-0675


Full Text: PDF

DOI: 10.33687/phytopath.015.01.5718

Refbacks

  • There are currently no refbacks.




Copyright (c) 2026 Marcos Javier Mendoza-Duarte, Milciades Ariel Melgarejo-Arrua, Andrea Celeste Zarza-Cabrera, Nery Rubén Villasboa-Cardozo, Marta Alicia Fernandez-Gamarra, Marco Maidana-Ojeda, Andrea Alejandra Arrua, Guillermo Andrés Enciso-Maldonado

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.