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Molecular Characterization of Botrytis cinerea Moroccan Isolates Infecting Grapevine and the Cryptic Species Status in North Africa/ Europe
Abstract
Grey mould is a serious disease that limits grapevine cultivation in some regions around the world. It causes severe losses in the quality and the volume of grapes harvested for wine production or fruit market. The genetic diversity of the Botrytis cinerea populations within Moroccan vineyards related to transposable elements (TEs) has been unexplored. Such information can help manage more effective strategies for controlling the disease. In the current study, 122 grapevine isolates were genotyped in order to check for transposable elements. It demonstrates that B. cinerea Moroccan populations is harbored all four transposable element states: both Boty and Flipper, Boty only, Flipper only and no transposable elements, relating to the four TEs genotypes Transposa, Boty, Flipper and Vacuma respectively. After frequency investigation, results showed that Transposa individuals containing Boty and Flipper TEs was the predominant type (78.69%) in grapevine populations, whereas only (2.46%) belonged to the Vacuma individuals lacking the two TEs types. Furthermore, Botrytis cinerea was mentioned to contain two cryptic species as species cluster, Groups I and II. Assessment of genetic polymorphism of the Bc-hch haplotyping locus using PCR and restriction enzyme digestion identified only the B. cinerea population isolates corresponding to one allelic type, so all the analysed isolates were classified as Group II B. cinerea stricto sensu (according to the most recent proposed classification). Study of genetic distribution of populations based on group I/group II cryptic species between North Africa and Europe studies reports revealed possible populations migration.
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References
Abdel Wahab, H. 2015. Characterization of Egyptian Botrytis cinerea isolates from different host plants. Advances in Microbiology, 5: 177–189. https://doi.org/10.4236/aim.2015.53017
Albertini, C., Thebaud, G., Fournier, E., and Leroux, P. 2002. Eburicol 14α-demethylase gene (CYP51) polymorphism and speciation in Botrytis cinerea. Mycological Research, 106: 1171–1178. https://doi.org/10.1017/S0953756202006561
Alfonso, C., Raposo, R., and Melgarejo, P. 2000. Genetic diversity in Botrytis cinerea populations on vegetable crops in greenhouses in south-eastern Spain. Plant Pathology, 49: 243–251. https://doi.org/10.1046/j.1365-3059.2000.00452.x
Amselem, J., Cuomo, C. A., van Kan, J. A. L., Viaud, M., Benito, E. P., Couloux, A., and Dickman, M. 2011. Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genetics, 7: e1002230. https://doi.org/10.1371/journal.pgen.1002230
Beever, R. E., and Weeds, P. L. 2007. Taxonomy and genetic variation of Botrytis and Botryotinia. In: Elad, Y., Williamson, B., Tudzynski, P., and Delen, N. (eds.), Botrytis: Biology, pathology and control. Springer, Dordrecht, Netherlands, pp. 29–52. https://doi.org/10.1007/978-1-4020-2626-3_3
Ben Ahmed, D., and Hamada, W. 2005. Genetic diversity of Tunisian Botrytis cinerea isolates using molecular markers. Phytopathologia Mediterranea, 44: 300. Available at: http://www.torrossa.it/gs/resourceProxy?an=2194567&publisher=FF3888
De Miccolis, M., Pollastro, S., and Faretra, F. 2016. Genetics of Botrytis cinerea. In: Botrytis – the fungus, the pathogen and its management in agricultural systems. Springer, Cham, Switzerland, pp. 35–53. https://doi.org/10.1007/978-3-319-23371-0_3
Dean, R., van Kan, J. A. L., Pretorius, Z. A., Hammond-Kosack, K. E., Di Pietro, A., Spanu, P. D., and Foster, G. D. 2012. The top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology, 13: 414–430. https://doi.org/10.1111/j.1364-3703.2011.00783.x
Diolez, A., Marches, F., Fortini, D., and Brygoo, Y. 1995. Boty, a long-terminal-repeat retroelement in Botrytis cinerea. Applied and Environmental Microbiology, 61: 103–108. Available at: http://dx.doi.org/10.1016/j.chemgeo.2013.02.014
Esterio, M., Muñoz, G., Ramos, C., Cofré, G., and Estévez, R. 2011. Characterization of Botrytis cinerea isolates in table grapes in Chile. Plant Disease, 95: 683–690. https://doi.org/10.1094/PDIS-04-10-0298
Fekete, É., Fekete, E., Irinyi, L., Karaffa, L., Árnyasi, M., Asadollahi, M., and Sándor, E. 2012. Genetic diversity of a Botrytis cinerea cryptic species complex in Hungary. Microbiological Research, 167: 283–291. https://doi.org/10.1016/j.micres.2011.10.006
Fournier, E., and Giraud, T. 2008. Sympatric genetic differentiation of Botrytis cinerea on different host plants. Journal of Evolutionary Biology, 21: 122–132. https://doi.org/10.1111/j.1420-9101.2007.01462.x
Fournier, E., Giraud, T., Albertini, C., and Brygoo, Y. 2005. Partition of the Botrytis cinerea complex in France. Mycologia, 97: 1251–1267. https://doi.org/10.3852/mycologia.97.6.1251
Fournier, E., Giraud, T., Loiseau, A., Vautrin, D., Estoup, A., Solignac, M., and Brygoo, Y. 2002. Characterization of polymorphic microsatellite loci in Botrytis cinerea. Molecular Ecology Notes, 2: 253–255. https://doi.org/10.1046/j.1471-8286.2002.00207.x
Fournier, E., Levis, C., Fortini, D., Leroux, P., Brygoo, Y., and Giraud, T. 2003. Characterization of Bc-hch in Botrytis cinerea. Mycologia, 95: 251–261. https://doi.org/10.2307/3762036
Giraud, T., Fortini, D., Levis, C., Lamarque, C., Leroux, P., Lobuglio, K., and Brygoo, Y. 1999. Two sibling species of Botrytis cinerea complex. Phytopathology, 89: 967–973. https://doi.org/10.1094/PHYTO.1999.89.10.967
Giraud, T., Fortini, D., Levis, C., Leroux, P., and Brygoo, Y. 1997. Genetic recombination in Botrytis cinerea. Molecular Biology and Evolution, 14: 1177–1185.
Harper, L. A., Derbyshire, M. C., and Lopez‐Ruiz, F. J. 2019. Identification of Botrytis medusae. Plant Pathology, 68: 1–15. https://doi.org/10.1111/ppa.13005
Isenegger, D. A., Ades, P. K., Ford, R., and Taylor, P. W. J. 2008. Status of Botrytis cinerea species complex. Fungal Diversity, 29: 17–26. Available at: http://www.fungaldiversity.org/fdp/sfdp/29-2.pdf
Johnston, P. R., Hoksbergen, K., Park, D., and Beever, R. E. 2014. Genetic diversity of Botrytis in New Zealand vineyards. Plant Pathology, 63: 888–898. https://doi.org/10.1111/ppa.12143
Karchani-Balma, S., Gautier, A., Raies, A., and Fournier, E. 2008. Genetic structure of Tunisian Botrytis cinerea. Phytopathology, 98: 1271–1279. https://doi.org/10.1094/PHYTO-98-12-1271
Kretschmer, M., and Hahn, M. 2008. Fungicide resistance and genetic diversity of Botrytis cinerea. Journal of Plant Diseases and Protection, 115: 214–219.
Kuzmanovska, B., Rusevski, R., Jankuloski, L., Jankulovska, M., Ivic, D., and Bandzo, K. 2012. Characterization of Botrytis cinerea isolates from tomato. Genetika, 44: 633–647. https://doi.org/10.2298/GENSR1203663K
Leroux, P., Fritz, R., Debieu, D., Albertini, C., Lanen, C., Bach, J., and Chapeland, F. 2002. Mechanisms of resistance to fungicides in Botrytis cinerea. Pest Management Science, 58: 876–888. https://doi.org/10.1002/ps.566
Levis, C., Fortini, D., and Brygoo, Y. 1997. Flipper transposable element in Botrytis cinerea. Molecular and General Genetics, 254: 674–680. https://doi.org/10.1007/s004380050465
Ma, Z., and Michailides, T. J. 2005. Genetic structure of Botrytis cinerea populations. Plant Disease, 89: 1083–1089. https://doi.org/10.1094/PD-89-1083
Martinez, F., Corio-Costet, M. F., Levis, C., Coarer, M., and Fermaud, M. 2008. PCR characterization of Botrytis cinerea populations. Vitis, 47: 217–226
Martinez, F., Dubos, B., and Fermaud, M. 2005. Role of saprotrophy in Botrytis cinerea. Phytopathology, 95: 692–700. https://doi.org/10.1094/PHYTO-95-0692
Möller, E. M., Bahnweg, G., Sandermann, H., and Geiger, H. H. 1992. DNA isolation protocol for fungi. Nucleic Acids Research, 20: 6115–6116. https://doi.org/10.1093/nar/20.22.6115
Muñoz, G., Hinrichsen, P., Brygoo, Y., and Giraud, T. 2002. Genetic characterization of Botrytis cinerea in Chile. Mycological Research, 106: 594–601. https://doi.org/10.1017/S0953756202005981
Peakall, R., and Smouse, P. E. 2006. GenAlEx 6: genetic analysis in Excel. Molecular Ecology Notes, 6: 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
Peakall, R., and Smouse, P. E. 2012. GenAlEx 6.5 update. Bioinformatics, 28: 2537–2539. https://doi.org/10.1093/bioinformatics/bts460
Rajaguru, B. A. P., and Shaw, M. W. 2010. Genetic differentiation in Botrytis cinerea. Plant Pathology, 59: 1081–1090. https://doi.org/10.1111/j.1365-3059.2010.02346.x
Samuel, S., Veloukas, T., Papavasileiou, A., and Karaoglanidis, G. S. 2012. Transposable elements in Botrytis cinerea. Plant Disease, 96: 1286–1290. https://doi.org/10.1094/PDIS-01-12-0103-RE
Tanovic, B., Hrustic, J., Mihajlovic, M., Grahovac, M., and Delibasic, G. 2014. Characterization of Botrytis cinerea in raspberry. Pesticidi i Fitomedicina, 29: 237–247. https://doi.org/10.2298/PIF1404237T
Topolovec-Pintaric, S., Milicevic, T., and Cvjetkovic, B. 2004. Genetic diversity of Botrytis cinerea. Journal of Plant Diseases and Protection, 111: 451–460. Available at: http://www.jstor.org/stable/43216279
Váczy, K. Z., Sándor, E., Karaffa, L., Fekete, E., Fekete, É., Árnyasi, M., and Kubicek, C. P. 2008. Sexual recombination in Botrytis cinerea. Phytopathology, 98: 1312–1319. https://doi.org/10.1094/PHYTO-98-12-1312
van Kan, J. A. L., Shaw, M. W., and Grant-Downton, R. T. 2014. Botrytis species biology. Molecular Plant Pathology. https://doi.org/10.1111/mpp.12148
Vercesi, A., Toffolatti, S. L., Venturini, G., Campia, P., and Scagnelli, S. 2014. Characterization of Botrytis cinerea populations. Phytopathologia Mediterranea, 53: 108–123. https://doi.org/10.14601/Phytopathol_Mediterr-12367
Walker, A.-S., Gautier, A. L., Confais, J., Martinho, D., Viaud, M., Le Pêcheur, P., and Fournier, E. 2011. Botrytis pseudocinerea identification. Phytopathology, 101: 1433–1445. https://doi.org/10.1094/PHYTO-04-11-0104
Yourman, L. F., Jeffers, S. N., and Dean, R. A. 2001. Phenotype instability in Botrytis cinerea. Phytopathology, 91: 307–315. https://doi.org/10.1094/PHYTO.2001.91.3.307
Zhou, Y. J., Zhang, J., Wang, X. D., Yang, L., Jiang, D. H., Li, G. Q., and Zhuang, W. Y. 2014. Identification of Botrytis sinoviticola. Mycologia, 106: 43–56. https://doi.org/10.3852/13-032
DOI: 10.33687/phytopath.014.03.5682
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