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Investigation of Relatedness between the Genetic Diversity and Disease Severity of Erwinia amylovora Strains Obtaining from Pears in Turkey

Aysegul GUR, Kubilay Kurtulus Bastas

Abstract


Erwinia amylovora, which causes fire blight disease, is one of the biggest threats to pome fruit growing worldwide and is one of the top ten most destructive bacterial pathogens. In this study, the relationships between the genetic diversity and disease severity of E. amylovora strains isolated from pears from different regions of Turkey were revealed by pEA29 plasmid DNA derived from A/B primers, amsG primers responsible for amylovoran production, CRISPR 1, 2 and 3 primers, used to characterize the genetic diversity and some properties of the pathogen. The findings indicated significant regional differences in percent disease severity and indices among pear strains. However, no genetic variation was observed in pEA29 and amylovoran production. Only one strain differed from the others with CRISPR 2 primers, and it was observed that the specific primers used to determine the relationship between regional differences and virulence of the strains were not completely sufficient to reveal the genetic difference. The results of the study indicated that E. amylovora is subject to changing ecological and climatic factors in different hosts, in different countries and regions. This scenario may have induced genetic heterogeneity among the examined strains, implying that variations in virulence could also emerge in strains harboring distinct plasmid DNAs. New perspectives can be developed in the control of the disease with a full understanding of the relationships between the pathogen's pathogenicity levels and genetic structure.

Keywords


Erwinia amylovora; Pear; CRISPR; Pathogenicity; Genetic diversity

References


Aldwinckle, H. S., Gustafson, H. L., Forsline, P. L., Bhaskara Reddy, M. V. 2001. Fire blight resistance of Malus species from Sichuan (China), Russian Caucasus, Turkey, and Germany. In IX International Workshop on Fire Blight 590 : 369-372.

FAO, 2025. Food and agriculture data. http://www.fao.org/faostat/en/#data/QC (Accessed : 02.03.2025).

Bastas K. K., 2011, Phenotypic and genetic diversity of Erwinia amylovora strains from different hosts. Current Opinion in Biotechnology, 22 (1): 133-133.

Bastas, K. K., Sahin, F. 2012a. First report of fire blight disease on blackberry in Turkey. Plant Disease, 96(12): 1818-1818.

Bastas, K. K., Sahin, F. 2012b. First report of fire blight disease caused by Erwinia amylovora on rockspray (Cotoneaster horizontalis) in Turkey. Plant Disease, 96(11), 1690-1690.

Bastas, K. K., Ozturk, A. Y. 2013. First report of fire blight caused by Erwinia amylovora on crabapple (Malus floribunda) in Turkey. Plant Disease, 97(9): 1244-1244.

Bastas, K. K., Sahin, F., Atasagun, R. 2013. First report of fire blight caused by Erwinia amylovora on rosehip (Rosa canina) in Turkey. Plant Disease, 97(12): 1652-1652.

Bereswill S, Pahl A, Bellemann P, Zeller W, Geider K.1992. Sensitive and species spesific-detection of Erwinia amylovora by polymerase chain reaction. Appl. and Env. Mic. 58: 3522-3526.

Bonasera, J. M., Kim, J. F., Beer, S. V. 2006. PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora. BMC Plant Biology, 6: 1-12.

Bonn WG, van der Zwet T. 2000. Distribution and economic importance of fire blight. In: Vanneste J (ed), Fire blight, the disease and its causative agent Erwinia amylovora. CABI publishing, Wallingford, UK:37-53.

Brennan, J. M., Doohan, F. M., Egan, D., Scanlan, H., & Hayes, D. 2002. Characterization and differentiation of Irish Erwinia amylovora isolates. Journal of Phytopathology, 150(8‐9), 414-422.

Chiou, C. S., Jones, A. L. 1993. Nucleotide sequence analysis of a transposon (Tn5393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria. Journal of Bacteriology, 175(3): 732-740.

Duzgunes, O., Kesici, T., Kavuncu, O., Gürbüz, F. 1987. Araştırma ve Deneme Metotları İstatistik Metotları II). Ankara Üni. Zir. Fak. Yayın No: 1021.

EPPO, 2023. PM 7/20 (3) Erwinia amylovora. EPPO Bull 2022(52): 198–224. https://doi.org/10.1111/epp.12826

EPPO, 2024. PM7/20(2)*Erwinia amylovora. EPPOBull43. 1: 21–45.

FAO, 2017. Food and agriculture data. http://www.fao.org/faostat/en/#data/QC (Acces date: 02.03.2019).

Fernando, W.G.D., Jones A. L., 1999. Pro hexadione-Ca A tool for reducing secondary fire blight infections. Acta Hort. 489: 597–600.

Gusberti, M., Klemm, U., Meier, M. S., Maurhofer, M., Hunger-Glaser, I. 2015. Fire blight control: the struggle goes on. A comparison of different fire blight control methods in Switzerland with respect to biosafety, efficacy and durability. International Journal of Environmental Research and Public Health, 12(9): 11422-11447.

Ivanovic, M., Obradović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R., & Jones, J. B. 2012. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns. European Journal of Plant Pathology, 133, 545-557.

Ibrahim, Y. E., Rafique, A. M., Al‐Masrahi, A. A., Al‐Saleh, M. A. 2024. Characterization of Erwinia amylovora isolates from the northern region of Saudi Arabia, including CRISPR genotyping. Plant Pathology, 73(8): 2193-2210.

Klement, Z., Goodman, R. N. 1967. The hypersensitive reaction to infection by bacterial plant pathogens. Annual Review of Phytopathology, 5(1), 17-44.

Koczan, J. M., McGrath, M. J., Zhao, Y., Sundin, G. W. 2009. Contribution of Erwinia amylovora exopolysaccharides amylovoran and levan to biofilm formation: implications in pathogenicity. Phytopathology, 99(11): 1237-1244

Krivokapic, M., Gavrilović, V., Ivanović, M., Kuzmanović, N., Fira, D., Obradović, A., & Gašić, K. (2018). Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. Pesticidi i fitomedicina, 33(3-4), 175-184.

Kurz, M., Carnal, S., Dafny-Yelin, M., Mairesse, O., Gottsberger, R. A., Ivanović, M., ... & Rezzonico, F. 2021. Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. Phytopathology Research, 3: 1-15.

Langlotz, C., Schollmeyer, M., Coplin, D.L., Nimtz, M. Geider, K. 2011. Biosynthesis of the repeating units of the exopolysaccharides amylovoran from Erwinia amylovora and stewartan from Pantoea stewartii. Physiological and Molecular Plant Pathology, 75: 163-169.

Lecomte, P., Manceau, C., Paulin, J.P., Keck, M. 1997. Identification by PCR analysis on plasmid pEA29 of Isolates of Erwinia amylovora responsible of an outbreak in Central Europe. European Journal of Plant Pathology, 103: 91-98.

Lelliott, R. A., Stead, D. E. 1987. Methods for the diagnosis of bacterial diseases of plants (pp. vii+-216pp).

McGhee, G. C., Sundin, G. W. 2011. Evaluation of kasugamycin for fire blight management, effect on nontarget bacteria, and assessment of kasugamycin resistance potential in Erwinia amylovora. Phytopathology, 101(2): 192-204

McGhee, G. C., Sundin, G. W. 2007. Thiamin biosynthesis and its influence on exopolysaccharide production: a new component of virulence identified on Erwinia amylovora plasmid pEA29. In XI International Workshop on Fire Blight, 793: 271-277.

McGhee, G. C., Sundin, G. W. 2012. Erwinia amylovora CRISPR elements provide new tools for evaluating strain diversity and for microbial source tracking. Plos One, 7(7): 41706.

McManus, P. S., Jones, A. L. 1995. Genetic fingerprinting of Erwinia amylovora strains isolated from tree-fruit crops and Rubus spp. Phytopathology, 85(12), 1547-1553.

Momol, M. T., Yeğen, O. 1993. Fire blight in Turkey: 1985-1992 Acta Horticulture 338.

Norelli, J. L., Aldwinckle, H. S., & Beer, S. V. 1984. Differential host x pathogen interactions among cultivars of apple and strains of Erwinia amylovora. Phytopathology, 74(2), 136-139.

Norelli, J. L., Jones, A. L., Aldwinckle, H. S. 2003. Fire blight management in the twenty-first century: using new technologies that enhance host resistance in apple. Plant Disease, 87(7): 756-765.

Öztürk, M., Kayaaslan, Z., 2024, Investigation of the genetic diversity of Erwinia amylovora strains originating from pome fruits in Turkey. Applied Fruit Science, 66 (2), 341-352.

Parcey, M., Gayder, S., Castle, A. J., Svircev, A. M. 2020. Molecular profile of phage infection: A novel approach for the characterization of Erwinia phages through qPCR. International Journal of Molecular Sciences, 21(2), 553.

Parcey, M., Gayder, S., Castle, A. J., Svircev, A. M. 2022. Function and application of the CRISPR-Cas system in the plant pathogen Erwinia amylovora. Applied and Environmental Microbiology, 88(7): e02513-21.

Pester, D., Milčevičová, R., Schaffer, J., Wilhelm, E., Blümel, S. 2012. Erwinia amylovora expresses fast and simultaneously hrp/dsp virulence genes during flower infection on apple trees. PLoS One, 7(3): 32583.

Popović, T., Jelušić, A., Živković, L., Živković, N., Iličić, R., Stanisavljević, R., Stanković, S. 2020. Identification, genetic characterization and virulence of Serbian Erwinia amylovora isolates. European Journal of Plant Pathology, 157: 857-872.

Puławska, J., Sobiczewski, P. 2012. Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight. Trees, 26: 3-12.

Rezzonico, F., Smits, T. H., Duffy, B. 2011. Diversity, evolution, and functionality of clustered regularly interspaced short palindromic repeat (CRISPR) regions in the fire blight pathogen Erwinia amylovora. Applied and Environmental Microbiology, 77(11): 3819-3829.

Rico, A., Ortiz‐Barredo, A., Ritter, E., & Murillo, J. 2004. Genetic characterization of Erwinia amylovora strains by amplified fragment length polymorphism. Journal of Applied Microbiology, 96(2): 302-310.

Schaad, N. W., Jones, J. B. and Chun, W. 2001. Laboratory guide for the identification of plant pathogenic bacteria, 3rd ed., APS Press.

Song, J. Y., Yun, Y. H., Kim, G. D., Kim, S. H., Lee, S. J., & Kim, J. F. 2021. Genome analysis of Erwinia amylovora strains responsible for a fire blight outbreak in Korea. Plant Disease, 105(4): 1143-1152.

Taylor, R. K., Guilford, P. J., Clark, R. G., Hale, C. N., & Forster, R. L. S. 2001. Detection of Erwinia amylovora in plant material using novel polymerase chain reaction (PCR) primers. New Zealand Journal of Crop and Horticultural Science, 29(1): 35-43.

Thomson, S.H. 2000. Epidemiology of fire blight. In: Vanneste J (ed), Fire blight, the disease and its causative agent Erwinia amylovora. CABI publishing, Wallingford, UK: 339-358.

Tokgönül, S., Çınar, Ö., 1991, Doğu Akdeniz bölgesinde armutlarda ateş yanıklığı hastalığı (Erwinia amylovora (Burr.) Winslow et al.)’nın tanısı ve yaygınlık durumu üzerinde araştırmalar. VI. Türkiye Fitopatoloji Kongresi Bildirileri, Ekim 7-11, İzmir, Türkiye. pp 303-306.

TÜİK, 2025. Crop production statistics database. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr, (Accessed: 03.12.2025).

Unlu, A., Basim, H. 2021. Characterization of Erwinia amylovora isolates causing fire blight disease by RAPD-PCR and SDS-page. Fresenius Environ Bull. 30(10):11548–11554.

Van der Zwet, T., and J. M. Wells, 1986: Determination of fatty acid profiles relevant to the characterization of Erwinia amylovora. Proc. 6th Intn. Conf. Plant Path. Bact. 821–829.

Van der Zwet T., 2006. Present worldwide distribution of fire blight and closely related diseases. Acta Hortic. 704:35–36

Vanneste, J. L. (Ed.). 2000. Fire blight: the disease and its causative agent, Erwinia amylovora. Cabi Publishing.

Wang, D., Korban, S. S., Pusey, P. L., Zhao, Y. 2012. AmyR is a novel negative regulator of amylovoran production in Erwinia amylovora. PLoS One, 7 (9): 45038.

Wang, D.P., Korban, S.S., Zhao, Y.F. 2009. The Rcs phosphorelay system is essential for pathogenicity in Erwinia amylovora. Molecular Plant Pathology, 10: 277-290.

Zwet, T., H. L. Keil, 1979. Fire Blight. A Bacterial Disease of Rosaceous Plants. Agriculturae Handbook. 510: 199.


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DOI: 10.33687/phytopath.014.02.5489

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