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Screening Indigenous Actinobacteria for Biocontrol of Ralstonia syzygii and Enhancing Chili Plant Growth
Abstract
Bacterial wilt caused by Ralstonia syzygii subsp. indonesiensis is an important disease of chili plants that is difficult to control. Actinobacteria derived from the rhizosphere of chili plants have the potential as biological control agents of R. syzygii subsp. indonesiensis. The purpose of the study was to obtain actinobacteria isolates that have the potential to control bacterial wilt disease in planta and increase the growth and production of chili plants. The research consisted of four stages: 1.) Isolation and characterization of actinobacteria isolates, 2.) Inhibition test of actinobacteria isolates from the rhizosphere of chili plants against R. syzygii subsp. indonesiensis in vitro 3). The ability of selected actinobacteria to control R. syzygii subsp. indonesiensis and increase the growth and production of chili plants 4). Molecular identification of selected actinobacteria from the rhizosphere of chili plants. The results obtained 21 isolates of actinobacteria from the rhizosphere of chili plants. After the biosafety test, 14 isolates could be used in the next test. Actinobacterial isolates that can suppress R. syzygii subsp. indonesiensis and produce enzymes and growth-promoting hormones in as many as 12 isolates. A total of 10 best actinobacterial isolates suppressing the development of R. syzygii subsp. indonesiensis are RZATABY 7.1, RZATABY 8.11, RZATABY 8.3, RZATABY 8.5, RZATABY 8.6, RZATABY 7.4, RZATBY 8.4, RZATBY 8.8, RZATPY 8.5, and RZATPY 8.6. The best actinobacteria isolate in increasing the growth and yield of chili plants was RZATPY 8.5, with an increase in plant height of 15.97 cm, an increase in the number of leaves 32 strands, accelerating the emergence of flowers 24.4 days and an increase in fresh fruit weight 126.33 g compared to control plants. 16sRNA sequence homology test results from the ten best actinobacteria isolates belong to Streptomyces spp. and Amicolatopsis sp. GLM4.
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DOI: 10.33687/phytopath.014.02.5015
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