In Vitro Exploration of Entomopathogenic Nematodes as Potential Biocontrol Agents of Brinjal Borer Leucinodes Orbonalis Guenée (Lepidoptera: Crambidae)

Muhammad Ataullah, Shahzab Riaz, Muhammad Tariq, Asad Aslam, Abdul Qadir, Adeel Asghar, Muhammad Shehzad, Muhammad Faheem Akhtar

Abstract


Leucinodes orbonalis is a potential pest of eggplant, commonly known as fruit and shoot borer. Owing to its devastating nature, chemical pesticides are frequently applied and insecticide pressure is significantly higher in developing nations. Keeping the current situation in mind the most effective and practical strategy for managing insect pests found in humid and subtropical environments is the bio-control. Entomopathogenic nematodes, has been utilized against many pests for their effective management. Keeping in view the biocidal potential of entomopathogenic nematodes, the current study was planned to explore the biocidal potential of Steinernema and Heterorhabditis, two significant genera of entomopathogenic nematodes against L. orbonalis. White trap extraction technique was used to isolate nematodes from several insect larvae. Four discrete concentrations of entomopathogenic nematodes viz. 60, 90, 120, and 150 infective juveniles per larvae were applied on larval stages. The virulence trial was followed by completely randomized block design. Multiple comparisons showed that among both the tested nematodes H. bacteriophora induced significant 81.33% mortality corresponding to 150 IJs/larva after 72 h of exposure as compared to S. glaseri. In the view of current research, entomopathogenic nematodes are recommended to be used in integrated pest management program for the management of insect pests.


Keywords


Entomopathogenic nematodes; Bio-control; Pesticide effects.

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References


Abd El Azim, A.M., 2022. Efficacy of the entomopathogenic nematode isolate Heterorhabditis taysearae to control the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control 32(1), 1-5.

Askary, T.H., Abd-Elgawad, M.M.M., 2017. Beneficial nematodes in agroecosystems: a global perspective. In Biocontrol agents: entomopathogenic and slug parasitic nematodes (pp. 3-25). Wallingford UK: CABI.

Bhat, A.H., Askary, T.H., Ahmad, M.J., Suman, Aasha, Chaubey, A.K., 2019. Description of Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae) isolated from hilly areas of Kashmir Valley. Egyptian Journal of Biological Pest Control, 29, 1-7.

Dowds, B.C., Peters, A.R.N.E., 2002. Virulence mechanisms. In: Entomopathogenic Nematology, Wallingford UK: CABI publishing, pp. 79-98.

Ebubekir, Y., Ramazan, C., 2018. Evaluation of local isolates of entomopathogenic nematodes for the management of black cutworm, Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control 28, 82.

Ehlers, R.U., 2001. Mass production of entomopathogenic nematodes for plant protection. Applied Microbiology and Biotechnology 56, 623-633.

Flores, P., Alvarado, A., Lankin, G., Lax, P., Prodan, S., Aballay, E., 2021. Morphological, molecular and ecological characterization of a native isolate of Steinernema feltiae (Rhabditida: Steinernematidae) from southern Chile. Parasites and Vectors 14, 1-13.

Friedman, M.J., 1990. Commercial production and development. Nematode Bio Control, pp. 153-172.

Goudarzi, M., Moosavi, M.R., Asadi, R., 2015. Effects of entomopathogenic nematodes, Heterorhabditis bacteriophora (Poinar) and Steinernema carpocapsae (Weiser), in biological control of Agrotis segetum (Lepidoptera: Noctuidae). Turkish Journal of Entomology 39(3), 239-250.

Gulzar, A., Mukhtar, T., Wright, D.J., 2020. Effects of entomopathogenic nematodes Steinernema carpocapsae and Heterorhabditis bacteriophora on the fitness of a Vip3A resistant subpopulation of Heliothis virescens (Noctuidae: Lepidoptera). Bragantia 79(2), 281-292.

Hominick, W.M., Briscoe, B.R., del Pino, F.G., Heng, J., Hunt, D.J., Kozodoy, E., Yoshida, M., 1997. Biosystematics of entomopathogenic nematodes: current status, protocols and definitions. Journal of Helminthology 71(4), 271-298.

Ibrahim, S.A.M., Salem, H.H.M.A., Taha, M.A., 2019. Dual application of entomopathogenic nematodes and fungi on immune and antioxidant enzymes of the greater wax moth, Galleria mellonella L. Egyptian Journal of Biological Pest Control 29, 20. https://doi.org/10.1186/s41938-019-0125-9

Javed, H., Mohsin, A.U., Aslam, M., Naeem, M., Amjad, M., Mahmood, T., 2011. Relationship between morphological characters of different aubergine cultivars and fruit infestation by Leucinodes Orbonalis Guenee. Pakistan Journal of Botany 43(4), 2023-2028.

Javed, H., Mukhtar, T., Javed, K., Mohsin, A.U., 2017. Management of eggplant shoot and fruit borer (Leucinodes orbonalis Guenee) by integrating different non-chemical approaches. Pakistan Journal of Agricultural Sciences 54(1), 65-70.

Javed, K., Javed, H., Mukhtar, T., Qiu, D., 2019. Efficacy of Beauveria bassiana and Verticillium lecanii for the management of whitefly and aphid. Pakistan Journal of Agricultural Sciences 56(3), 669-674

Kassi, A.K., Javed H., Mukhtar, T., 2019. Screening of different aubergine cultivars against infestation of brinjal fruit and shoot borer (Leucinodes orbonalis Guenee). Pakistan Journal of Zoology 51(2), 603-609.

Kaya, H.K., Gaugler, K., 1993. Entomopathogenic nematodes. Annual Review of Entomology 38(1), 181-206.

Khanum, T.A., Javed, S., 2020. Virulence of four Steinernema species as a biological control agent in controlling the termite, Coptotermes heimi (Wasmann) (Isoptera: Rhinotermitidae). Egyptian Journal of Biological Pest Control 30(1), 1-4.

Muhammad, W., Javed, H., Ahmad, M., Mukhtar. T., 2021. Economic impact of some selected cultural practices on population build-up of Leucinodes orbonalis in brinjal crop. Fresenius Environmental Bulletin 30(06B), 7346-7354.

Mutegi, D.M., Dora, K., John, W.K., Charles, W., 2018. Integrated use of Kenyan entomopathogenic nematodes (Steinernema species) and neem against Tuta absoluta on tomato. International Journal of Research in Agricultural Sciences (4), 2348-3997.

Poinar, G.O., 1990. Biology and taxonomy of steinernematidae and heterorhabditidae. In Entomopathogenic nematodes in biological control, pp. 23-61.

Rahoo, A.M., Mukhtar, T., Abro, S.I., Bughio, B.A., Rahoo, R.K., 2018b. Comparing the productivity of five entomopathogenic nematodes in Galleria mellonella. Pakistan Journal of Zoology 50(2), 679-684.

Rahoo, A.M., Mukhtar, T., Bhugio, B.K., Rahoo, R.K., 2019a. Comparison of infectivity and productivity of Steinernema feltiae and Heterorhabditis bacteriophora in Galleria mellonella and Tenebrio molitor. Pakistan Journal of Zoology 51(2), 717-724.

Rahoo, A.M., Mukhtar, T., Bughio, B.K., Rahoo, R.K., 2019b. Relationship between the size of Galleria mellonella larvae and the production of Steinernema feltiae and Heterorhabditis bacteriophora. Pakistan Journal of Zoology 51(1), 79-84.

Rahoo, A.M., Mukhtar, T., Gowen, S.R., Pembroke, D.B., 2011. Virulence of entomopathogenic bacteria Xenorhabdus bovienii and Photorhabdus luminescens against Galleria mellonella larvae. Pakistan Journal of Zoology 43(3), 543-548.

Rahoo, A.M., Mukhtar, T., Gowen, S.R., Rahoo, R.K., Abro, R.I., 2017. Reproductive potential and host searching ability of entomopathogenic nematode, Steinernema feltiae. Pakistan Journal of Zoology 49(1), 229-234.

Rahoo, A.M., Mukhtar, T., Jakhar, A.M., Rahoo, R.K., 2018a. Inoculum doses and exposure periods affect recovery of Steinernema feltiae and Heterorhabditis bacteriophora from Tenebrio molitor. Pakistan Journal of Zoology 50(3), 983-987.

Reddy, G.R.S., Devakar, B.H., 1997. Effects of two microbial bio-agents on tomato borer, Helicoverpa armigera. Microbial Technology Journal 7(2), 140-142.

Rostaee, A.B., Adam, N.A., Jamian, S., 2021. Life cycle and determination of larval instars of Leucinodes orbonalis Guenee (Lepidoptera: Crambidae). Serangga 26(2), 166-182.

Sankar, M., Sethuraman, V., Palaniyandi, M., Prasad, J.S., 2009. Entomopathogenic nematode-Heterorhabditis indica and its compatibility with other biopesticides on the greater wax moth-Galleria mellonella (L.). Indian Journal of Science and Technology 2(1), 57-62.

Shehzad, M., Bodlah, I., Siddiqui, J.A., Bodlah, M.A., Fareen, A.G E., Islam, W., 2023. Recent insights into pesticide resistance mechanisms in Plutella xylostella and possible management strategies. Environmental Science and Pollution Research 1-16.

Shehzad, M., Tariq, M., Ali, Q., Aslam, A., Mukhtar, T., Akhtar, M.F., Gulzar, A., Faisal. M., 2022. Evaluation of insecticidal activity of Beauveria bassiana against different instar larvae of Plutella xylostella by using two different methods of application. International Journal of Tropical Insect Science 42, 1471-1476.

Shehzad, M., Tariq, M., Mukhtar, T., Gulzar, A., 2021. On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). Egyptian Journal of Biological Pest Control 31(1), 86.

Shirale, D., Patil, M., Parimi, S., 2017. Insecticide resistance in field populations of Leucinodes orbonalis (Lepidoptera: Crambidae) in India. The Canadian Entomologist 149(3), 399-407.

Visnupriya, M., Muthukrishnan, N., 2017. Negative cross resistance of Leucinodes orbonalis population of brinjal to newer molecule spinetoram 12 SC W/V (11.7 W/W). International Journal of Current Microbiology and Applied Sciences 6(12), 1790-1796.

White, G.F., 1927. A method for obtaining infective nematode larvae from cultures. Science (Washington), 66.

Woodring, J.L., Kaya, H.K., 1988. Heterorhabditis and Steinernema nematodes: A handbook of biology and techniques. Southern Cooperative Series Bulletin USA. 331.

Xu, Y., Liu, H., Li, F., Sun, N., Ren, Y., Liu, Z., Cao, X., Wang, Y., Liu, P., Zhang, K., 2010. A polymorphism in the micro RNA-30 precursor associated with major depressive disorder risk and P300 waveform. Journal of Affective Disorders 127(1-3), 332-336.




DOI: https://doi.org/10.33804/pp.008.01.5027

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