In Vitro Conservation of Prunus Avium, an Endangered Species in Tunisia, Using a Plant Extract of Rubus Fruticosus Compared to Indole-3-Butyric Acid

Jdaidi Nouri, Houcine Selmi, Foued Aloui, Abbes Chaabane

Abstract


Prunus avium L. is the rarest forest fruit tree in northwestern Tunisia, with strong socio-economic, agronomic, and commercial potential. The aim of this study was to provide more information about the domestication of this species by using an inexpensive in vitro culture technique for micropropagation of meristems. The plant extract used in this study was Rubus fruticosus. The results showed that the best concentration of the extract of R. fruticosus applied for the budding of meristematic microcuttings was 15 g/L, with a 100% success rate. The maximum number of adventitious shoots (7.15 ± 0.35) was obtained for the meristematic microcuttings at a concentration of 15 g/L of the extract. These results indicated that the extracted R. fruticosus plant promotes the neoformation of adventitious shoots and leaves. Rhizogenesis was strongly favored by the addition of 1 mg/L of IBA and 15 g/L of plant extract to the MS medium, which leads to the formation of roots for the meristematic micro-cuttings with percentages of about 80.36% and 53.32%, respectively. The current investigation proved that in vitro micropropagation could constitute a good alternative to the regeneration of this rare forest fruit species and may be applicable to other endangered plants.


Keywords


Prunus avium; micropropagation; in vitro; Rubus fructicosus; Indole-3-butyric acid.

Full Text:

PDF

References


Abdalla M.M., Mostafa, R.A.A., 2015. In vitro propagation of Blackberry (Rubus fruticosus L.). Assiut Journal of Agricultural Sciences 46(3), 88-99.

Acero, N., Gradillas, A., Beltran, M., Garcia, A., Mingarro, D.M., 2019. Comparison of phenolic compounds profile and antioxidant properties of different sweet cherry (Prunus avium L.) varieties. Food chemistry 279, 260-271.

Aghaye, R.N.M., Yadollahi, A., Moeini, A., Sepahvand, S., 2013. In vitro culture of Gisela 6 semi-dwarf rootstock. Journal of Environmental Sciences 7, 57-64.

Aly, A.A., El-Desouky, W., El-Leel, O.F.A., 2022. Micropropagation, phytochemical content and antioxidant activity of gamma-irradiated blackberry (Rubus fruticosus L.) plantlets. In vitro Cellular and Developmental Biology-Plant 58, 457-469.

Arikan, S., Ipekand, M., Esitken, A., 2014. In vitro micropropagation of Blackberry (Rubus fruticosus.) Cultivar “Jumbo”. Australian Journal of Industry Research SCIE Journals, a special issue for the International Conferences held by the Australian Society for Commerce Industry and Engineering (SCIE) in Dubai during the 15-16 November.

Clapa, D., Fira, A., Joshee, N., 2013. An efficient Ex vitro rooting and acclimatization method for horticultural plants using float hydroculture. Hortscience 48(9), 1159-1167.

Debnath, S.C., Vyas, P., Goyali, J.C., Igamberdiev, A.U., 2012. Morphological and molecular analyses in micropropagated berry plants acclimatized under ex vitro condition. Canadian Journal of Plant Science 92, 1065-1073.

Domínguez-Rodríguez, G., Marina, M.L., Plaza, M., 2021. Enzyme-assisted extraction of bioactive non-extractable polyphenols from sweet cherry (Prunus avium L.) pomace. Food chemistry 339, 128086.

Fira, A., Clapa, D., Plopa, C., 2009. Micropropagation cultivar “Thornless evergreen”. proceedings RIFG Piteşti 145, 213-221.

Fira, A., Clapa, D., Simu, M., 2014. Studies regarding the Micropropagation of Some Blackberry Cultivars. Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture 71, 29-37.

Gao, X., Yang, D., Cao, D., Ao, M., Sui, X., Wang, Q., Kimatu, J.N., Wang, L., 2010. In vitro micropropagation of Freesia hybrid and the assessment of genetic and epigenetic stability in regenerated plantlets. Journal of Plant Growth Regulation 29, 257-267.

García-Gonzáles, R., Quiroz, K., Carrasco, B., Caligari, P., 2010. Plant tissue culture: current status, opportunities and challenges. International Journal of Agriculture and Natural Resources 37, 5-30.

Gururaj, H.B., Giridhar, P., Ravishankar, G.A., 2007. Micropropagation of Tinospora cordifolia (Willd.)Miers ex Hook.F & Thoms- a multipurpose medicinal plant. Current Science 92, 23-26.

Hasan, S.Z.U., Ahmad, T., Hafiz, I.A., Hussain, A., 2010. Direct plant regeneration from leaves of Prunus rootstock GF-677 (Prunus amygdalus x P. persica). Pakistan Journal of Botany 42, 3817-3830.

Jdaidi, N., Aloui, F., Selmi, H., Abbes, C., 2021. Proliferation and rooting tests of Prunus avium root segments cuttings: Effect of auxin and cutting date. Algerian Journal of Arid Environment 11(1), 124-131.

Jdaidi, N., Selmi, H., Aloui, F., Chaabane, A., 2022. Spatial and diametric evolution of a rare species, Prunus avium, in different biotopes in northwest Tunisia. Journal of Applied Life Sciences and Environment 55 (3), 363-376.

Katičić Bogdan, I., Švorinić, K., Bogdan, S., Kajba, D., 2015. Generative and vegetative activity of wild cherry (Prunus avium L.) in a clonal seed orchard. Šumarski List 139(7-8), 339-348.

Ko, C., Al-Abdulkarim, A.M., Al-Jowid, S.M. and Al-Baiz, A., 2009. An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry. African Journal of Biotechnology, 8(11), 2611–2615.

Kumar, A., Sharma, V., Thakur, M., 2020. In vitro rooting and hardening of clonal cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens). International Journal of Agricultural Sciences 90 (5), 1032-1035.

Ljubojević, M., Ognjanov, V., Zorić, L., Maksimović, I., Merkulov, L.J., Bosnjakovic, D., Barac, G., 2013. Modeling of water movement trough cherry plant as preselectingtool for prediction of treevigor. Scientia Horticulturae-Amsterdam 160, 189-197.

Meier-Dinkel, A., 2007. Cryopreservation of Betula in vitro shoot tips by vitrification. Advances in Horticultural Science, 215-218.

Mir, J.I., Ahmed, N., Verma, M.K., Ahmad, M., Lal, S., 2010. In-vitro multiplication of cherry rootstocks. Indian Journal of Horticulture 67(4), 29-33.

Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 5, 473-497.

Naddaf, M.E., Rabiei, G., Ganji Moghadam, E., Mohammadkhani, A., 2021. In vitro Production of PPV-free Sweet cherry (Prunus avium cv. Siahe-Mashhad) by Meristem culture and micrografting. Journal of Plant Bioinformatics and Biotechnology 1(1), 51-59.

Najaf-Abadi, A.J., Hamidoghli, Y., 2009. Micropropagation of Thornless trailing blackberry (Rubus sp.) by axillary bud explants. Australian Journal of Crop Science 3 (4), 191-194.

Pamfil, D., Zimmerman, R.H., Naess, S.K., Swartz, H.J., 2000. Investigation of Rubus breeding anomalies and taxonomy using RAPD analysis. Small Fruits Review 1, 43-56.

Quambusch, M., Brümmer, J., Haller, K., Winkelmann, T., Bartsch, M., 2016. Dynamics of endophytic bacteria in plant in vitro culture: Quantification of three bacterial strains in Prunus avium in different plant organs and in vitro culture phases. Plant Cell, Tissue and Organ Culture 126, 305-317.

Quambusch, M., Gruß, S., Pscherer, T., Winkelmann, T., 2017. Improved in vitro rooting of Prunus avium microshoots using a dark treatment and an auxin pulse. Scientia Horticulturae 220, 52-56.

Russel, K., 2003.Wild cherry (Prunus avium). EUFORGEN Technical Guidelines for Genetic Conservation and Use, 6 p. ISBN: 978-92-9043-572-3, ISBN: 92-9043-572-0

Ruzic, D., Lazic, T., 2006. Micropropagation as means of rapid multiplication of newly developed blackberry and black currant cultivars. Agriculturae Conspectus Scientificus 71(4), 149-153.

Ružić, D.V., Vujovic, T.I., 2008. The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). HortScience (Prague) 35(1) , 12-21.

Sarropoulou, V., Dimassi-Theriou, K., Therios, I., 2013. Indole-3-butyric acid and myo-inositol impacts on in vitro rooting of the cherry rootstocks CAB-6P and Gisela 6. Plant Biology 57- 613-619.

Sharma, H., Vashistha, B.D., 2015. In vitro plant regeneration through callus in Giloy (Tinospora cordifolia (Willd.) Miers ex Hook.f&Thoms.). Indian Journal of Science and Technology (34), 59-68.

Tančeva Crmarić, O., Kajba, D., 2016. Micropropagation of wild cherry (Prunus avium L.) from a clonal seed orchard. Šumarski list 140(5-6), 273-281.

Vujović, T., Ružić, D.J., Cerović Vujovic, R., Ruzic, D., Cervoic, R., 2012. In vitro multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. HorticSci 39, 101-107.

Xi, Y., Zeng, B., Huang, H., Wang, Y., Yang, P., 2022. Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. ‘Fuchen’. HortScience (Prague) 49, 1-9.

Zamanipour, M., Moghaddam, E.G., Tehranifar, A., Abedi, B., 2019. Meristem culture of Prunus avium cvs." Siyah-e-Mashhad" and" Takdane". Azarian Journal of Agriculture, 6(1), 13-18.

Zhou, H., Li, M., Zhao, X., Fan, X., Guo, A., 2010. Plant regeneration from in vitro leaves of the peach rootstock ‘Nemaguard’ (P. persica× P. davidiana). Plant Cell, Tissue and Organ Culture 101(1), 79-87.




DOI: https://doi.org/10.33804/pp.008.01.4974

Refbacks

  • There are currently no refbacks.