Evaluating the Biocontrol Efficacy of Selected Botanical Extracts against Bacterial Spot of Tomato

Basharat Mehmood, Muhammad Jamil, Saima Shafique, Muhammad Riaz Khan, Tehmina Zafar, Rafia Munir Bhatti, Hassam Qayyum, Kinza Tasneem, Muhammad Tahir Younas, Muhammad Abu Bakar


Tomato crops are vulnerable to various bacterial diseases, including bacterial spot, which is caused by Xanthomonas perforans. This bacterial pathogen can have a severe impact on tomato plants, leading to lesions and spots on leaves, stems, and fruits. Consequently, it diminishes both yield and quality. The objective of the current study is to assess the efficacy of extracts derived from Cannabis sativa, Berberis vulgaris, and Azadirachta indica in combating bacterial spot disease in tomato plants. These plant extracts were examined for their potential as natural biocontrol agents, aimed at curbing the bacterial spot pathogen and reducing disease incidence. The antibacterial activity of selected aqueous plant extracts was tested using three concentrations (5%, 10%, and 15%) against X. perforans. The inhibition zone technique yielded significant results. Among the tested plant extracts, the highest inhibition zones were observed using C. sativa extract at concentrations of 15% and 10%, resulting in zones of 15 mm and 13 mm, respectively. Similarly, in a greenhouse experiment, the 15% concentration of C. sativa exhibited the most effective treatment. Among the three tested plant extracts, C. sativa demonstrated the highest efficacy in terms of Area under the Disease Progress Curve (AUDPC) severity, with values of 59.4%, 55.6%, and 51.3% observed at concentrations of 5%, 10%, and 15%, respectively. The findings of the study suggest that the plant extracts possess significant antibacterial activity, rendering them potentially valuable as effective components in integrated management programs for the sustainable control of tomato bacterial spot disease.


Plant Extracts; Berberis vulgaris; Bacterial spot; Tomato

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Abo-Elyousr, K., Asran, M., 2009. Antibacterial activity of certain plant extracts against bacterial wilt of tomato. Archives of Phytopathology and Plant Protection 42, 573-578.

Abo-Elyousr, K.A., El-Hendawy, H.H., 2008. Integration of Pseudomonas fluorescens and acibenzolar-S-methyl to control bacterial spot disease of tomato. Crop Protection 27, 1118-1124.

Abrahamian, P., Sharma, A., Jones, J.B., Vallad, G.E., 2021. Dynamics and spread of bacterial spot epidemics in tomato transplants grown for field production. Plant Disease 105, 566-575.

Aiello, D., Scuderi, G., Vitale, A., Firrao, G., Polizzi, G., Cirvilleri, G., 2013. A pith necrosis caused by Xanthomonas perforans on tomato plants. European Journal of Plant Pathology 137, 29-41.

Bashir, A., Khan, M.T., Ahmed, R., Mehmood, B., Younas, M.T., Rehman, H.M., Hussain, S., 2020. Efficiency of selected botanicals against (Alternaria solani) causing early blight disease on tomato in Azad Jammu and Kashmir. Pakistan Journal of Phytopathology 32, 179-186.

Bibi, S., Inam-ul-Haq, M., Riaz, A., Malik, S.I., 2022. Identification of host resistance against bacterial canker disease on apricot, plum and peach grown in Punjab and KPK. International Journal of Phytopathology 11, 43-48.

Bibi, S., Inam-Ul-Haq, M., Riaz, A., Malik, S.I., Tahir, M.I., Ahmed, R., 2017. Screening and characterization of Rhizobacteria antagonistic to Pseudomonas syringae causing bacterial canker of stone fruits in Punjab and KPK. International Journal of Biosciences 10, 405-412.

Elbeshehy, E.K., Elazzazy, A.M., Aggelis, G., 2015. Silver nanoparticles synthesis mediated by new isolates of Bacillus spp., nanoparticle characterization and their activity against Bean Yellow Mosaic Virus and human pathogens. Frontiers in Microbiology 6, 453-462.

Fallanaj, F., Sanzani, S.M., Youssef, K., Zavanella, C., Salerno, M.G., Ippolito, A., 2015. A new perspective in controlling postharvest citrus rots: The use of electrolyzed water. Acta Hortic 1065, 1599-1605.

FAO, 2021. World Food and Agriculture-Statistical Yearbook 2021. Food and Agriculture Organization, Rome, Italy.

Hassan, M.E., Abd El-Rahman, S.S., El-Abbasi, I., Mikhail, M., 2007. Changes in peroxidase activity due to resistance induced against faba bean chocolate spot disease. Egyptian Journal of Phytopathology 35, 35-48.

Hyder, S., Gondal, A., Ahmed, R., Sahi, S., Rehman, A., Hannan, A., 2018a. First report of charcoal rot in tomato caused by Macrophomina phaseolina (Tassi) Goid. from Pakistan. Plant Disease 102, 1459.

Hyder, S., Naseem, S., Azhar, S., Ashfaq, M., Ali, Z., Khalid, A., Inam-ul-Haq, M., 2018b. Disease incidence and severity of Pythium spp. and Phytophthora spp. affecting chili pepper and tomato crops in Punjab, Pakistan. Philippine Agricultural Scientist 101, 36-44.

Inam-ul-Haq, M., Naqvi, S.F., Tahir, M.I., Rehman, H.M., Ahmed, R., Ali, Z., 2016. Serodiagnosis of Xanthomonas campestris pv. sesami causing bacterial leaf blight disease in sesame. Pakistan Journal of Agricultural Sciences 53, 535-539.

Jabeen, Z., Riaz, A., Naz, F., Ahmed, M.S., Raheel, A., 2022. Evaluation of antifungal potential of indigenous plant extracts against grey mould and HPLC and LC-MS based identification of phytochemical compounds in Polygonum amplexicaule D. Don extracts. International Journal of Phytopathology 11, 287-299.

Jones, J.B., Miller, S.A., 2014. Bacterial Leaf Spot, in: Jones, J.B., Zitter, T.A., Momol, M.T., Miller, S.A. (Eds.), Compendium of Tomato Diseases and Pests. APS Press, St. Paul, USA.

Kagale, S., Marimuthu, T., Thayumanavan, B., Nandakumar, R., Samiyappan, R., 2004. Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiological and Molecular Plant Pathology 65, 91-100.

Klaus-Joerger, T., Joerger, R., Olsson, E., Granqvist, C.-G., 2001. Bacteria as workers in the living factory: Metal-accumulating bacteria and their potential for materials science. Trends in Biotechnology 19, 15-20.

Kumar, A.S., Abyaneh, M.K., Gosavi, S., Kulkarni, S.K., Pasricha, R., Ahmad, A., Khan, M., 2007. Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO 3. Biotechnology Letters 29, 439-445.

Manik, U., Nande, A., Raut, S., Dhoble, S., 2020. Green synthesis of silver nanoparticles using plant leaf extraction of Artocarpus heterophylus and Azadirachta indica. Results in Materials 6, 100086.

Mbega, E.R., Mortensen, C.N., Mabagala, R.B., Wulff, E.G., 2012. The effect of plant extracts as seed treatments to control bacterial leaf spot of tomato in Tanzania. Journal of General Plant Pathology 78, 277-286.

Mehmood, B., Abbas, M., Hussain, S., Rahim, N., Shafique, S., Khan, R.T., Zafar, T., Bhatti, R.M., Bakar, A., Younas, M.T., 2023. Exploring the Potential of Green Silver Nanoparticles from Berberis vulgaris against Bacterial Spot of Tomato and its Surveillance in Poonch District. International Journal of Phytopathology 12, 49-61.

Mehmood, B., Azad, A., Rahim, N., Arif, S., Khan, M.R., Hussain, A., Tariq-Khan, M., Younis, M.T., Bashir, A., Ahmed, S., 2022. Management of late blight of potato caused by Phytophthora infestans through botanical aqueous extracts. International Journal of Phytopathology 11, 35-42.

Mello, S., Takatsu, A., Lopes, C., 1997. Escala diagramática para avaliação da mancha-bacteriana do tomateiro. Fitopatologia Brasileira 22, 447-448.

Perveen, R., Suleria, H.A.R., Anjum, F.M., Butt, M.S., Pasha, I., Ahmad, S., 2015. Tomato (Solanum lycopersicum) carotenoids and lycopenes chemistry; metabolism, absorption, nutrition, and allied health claims—A comprehensive review. Critical Reviews in Food Science and Nutrition 55, 919-929.

Shahzaman, S., Gardazi, R.A., Inam-ul-Haq, M., Mastoi, A.H., Hayat, S., Ahmed, R., 2017. Optimization of nutritional conditions and effect of time and temperature for the production of bacterial biocontrol agents. International Journal of Biosciences 11, 141-146.

Shahzaman, S., Inam-Ul-Haq, M., Bibi, S., Sufyan, M., Altaf, A., Mehmood, U., Ahmed, R., 2016. Bio-efficacy of Pseudomonas fluorescens isolated from chickpea fields as plant growth promoting rhizobacteria. International Journal of Biosciences 9, 138-146.

Shahzaman, S., Inam-ul-Haq, M., Mukhtar, T., Naeem, M., 2015. Isolation, identification of antagonistic rhizobacterial strains obtained from chickpea (Cicer arietinum L.) field and their in-vitro evaluation against fungal root pathogens. Pakistan Journal of Botany 47, 1553-1558.

Shakoor, S., Inam-ul-Haq, M., Bibi, S., Ahmed, R., 2015. Influence of root inoculations with vasicular arbuscular mycorrhizae and rhizomyx for the management of root rot of chickpea. Pakistan Journal of Phytopathology 27, 153-158.

Srivastava, S., Singh, V., Kumar, R., Srivastava, M., Sinha, A., Simon, S., 2011. In vitro evaluation of carbendazim 50% WP, antagonists and botanicals against Fusarium oxysporum f. sp. psidii associated with rhizosphere soil of guava. Asian Journal of Plant Pathology 5, 46-53.

Stall, R.E., Jones, J.B., Minsavage, G.V., 2009. Durability of resistance in tomato and pepper to xanthomonads causing bacterial spot. Annual Review of Phytopathology 47, 265-284.

Vicente, J., Roberts, S., 2003. Screening wild cherry micropropagated plantlets for resistance to bacterial canker, Pseudomonas syringae and related pathogens: Biology and genetic. Springer, pp. 467-474.

DOI (PDF): https://doi.org/10.33804/pp.007.02.4726


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