Marcoandre Savaris, Sandra M. Sabedot-Bordin, Caroline E. Mendes, Neusa F. Moura, Cristiano M. Teixeira, Flavio R.M. Garcia


The maize weevil, Sitophilus zeamais is a major pest of stored grain whose control has been performed with the use of synthetic insecticides, which in many cases are being banned due to issues involving risks to human and animal health and to the environment. In order to avoid the use of these insecticides, alternative control has been sought, including the use of plant extracts. In this study, “poejo”, Cunila angustifolia leaves extracts and essential oils have been used against S. zeamais. The results of the bioassay for the control of S. zeamais showed statistical significance (P <0.05) for all sources of variation. The essential oil reached 100% efficiency at all different concentrations, the hexane extract at 0.32% showed 38.70% efficiency, while the aqueous and crude extracts showed no insecticidal effect on adults of S. zeamais. The highest mortality rate was found after 96 h of application of the extracts.


Weevil, insecticide, pest, stored, grains

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Abbot, W.S. 1925. A method of computing the effectiveness of insecticide. Journal of Economic Entomology, 18(2): 265-267.

Alves, W.V., E.R. Lorenzetti and F.C. Gonçalves. 2012. Utilização de acaricidas a base de plantas no controle de Rhipicephalus (Boophilus) microplus: Uma contribuição para a produção e desenvolvimento sustentável. RevistaBrasileira de Agropecuária Sustentável, 2(2): 14-25.

Apel, M.A., V.L.S. Ribeiro, S.A.L. Bordignon, A.T Henriques and G.L. von Poser. 2009. Chemical composition and toxicity of the essential oils from Cunila species (Lamiaceae) on the cattle tick Rhipicephalus (Boophilus) microplus. Parasitology Research, 105(3): 863–868.

Bordignon, S.A.L., E.P. Schenkd and V. Spitzer. 1997. The essential oil composition of Cunila microcephala and Cunila fasciculata. Phytochemistry, 44(7): 1283-1285.

Bordignon, S.A.L., E.P. Schenkd and V. Spitzer. 1999 . The Essential Oil Composition of Cunila angustifolia (Lamiaceae). Journal of Essential Oil Research, 11(2): 145-148.

Cavalcante, G.M., A.F.C. Moreira and S.D. Vasconcelos. 2006. Potencialidade inseticida de extratos aquosos de essências florestais sobre mosca-branca. Pesquisa agropecuária brasileira, 41(1): 9-14.

Dayan, F.E., C.L. Cantrell and S.O. Duke. 2009. Natural products in crop protection. Bioorganic & Medicinal Chemistry, 17(12): 4022–4034.

Echeverrigaray, S., F. Fracaro, A.C.A. Santos, N. Paroul, R. Wasum and L.A. Serafini. 2003. Essential oil composition of south Brazilian populations of Cunila galioides and its relation with the geographic distribution. Biochemical systematics and ecology, 31(5): 467-475.

Echeverrigaray, S., J. Zacaria and R. Beltrão. 2010. Nematicidal activity of monoterpenoids against the root knot nematode Meloidogyne incognita. Phytopathology, 100(2): 199-203.

Garcia, F.R.M. 2014. Zoologia Agrícola. 4ª ed. Ed. Rígel, Porto Alegre, Brasil.

Gazima, Z.C., I.G. Demarchi, M.V.C. Lonardoni, A.C.L. Amorim, A.M.C. Hovell, C.M. Rezende, G.A. Ferreira, E.L. Lima, F.A. Cosmo and D.A.G. Cortez. 2011. Acaricidal activity of the essential oil from Tetradenia riparia (Lamiaceae) on the cattle tick Rhipicephalus (Boophilus) microplus (Acari; Ixodidae). Experimental Parasitology, 129(2): 175-180.

Gordon, W.P., A.C. Huitric, C.L. Seth, R.H. Mcclanahan and S.D. Nelson. 1987. The metabolism of the abortifacient terpene, (R)-(+)-pulegone, to a proximate toxin, (R)-(+)-menthofuran. Drug Metabolism and Disposition, 15(5): 589-594.

Gordon, W.P., A.J. Forte, R.J. Mcmurty, H. Gal and S.D. Nelson. 1982. Hepatotoxicity and pulmonary toxicity of pennyroyal oil and its constituent terpenes in the mouse. Toxicology and Applied Pharmacology, 65(3): 413-424.

Jbilou, R., A. Ennabili and F. Sayah. 2006. Insecticidal activity of four medicinal plant extracts against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). African Journal of Biotechnology, 5(10): 936-940.

Lovatto, P.B., G. Schiedeck and F.R. M. Garcia. 2012. A interação co-evolutiva entre insetos e plantas como estratégia ao manejo agroecológico em agroecossistemas sustentáveis. Interciencia, 37(9): 657-663.

Maedeh, M., I. Hamzeh, D. Hossein, A. Majid and R.K. Reza. 2011. Bioactivity of essential oil from Satureja hortensis (Lamiaceae) against three stored-product insect species. African Journal of Biotechnology, 10(34): 6620-6627.

Manns, D. 1995. Linalool and cineole type glucosides from Cunila spicata. Phytochemistry, 39(5): 1115-1118.

Marangoni, C., N.F. de Moura and F.R. M. Garcia. 2012. Utilização de óleos essenciais e extratos de plantas no controle de insetos. Revista de Ciências Ambientais, 6(2): 95-112.

Papachristos, D.P. and D.C. Stamoupolous. 2002a. Repellent, toxic and reproduction inhibitory effects of essential oil vapours on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Product Research, 38(2):117 128.

Papachristos, D.P. and D.C. Stamoupolous. 2002b. Toxicity of vapours of three essential oils to the immature stages of Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Product Research, 38(4): 365-373.

Papachristos, D.P. and D.C. Stamoupolous. 2004. Fumigation toxicity of three essential oil on the eggs of Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Product Research, 40(5): 517-525.

Pirali-Kheirabadia, K. and J.A.T. Silva. 2010. Lavandula angustifolia essential oil as a novel and promising natural candidate for tick Rhipicephalus (Boophilus) annulatus control. Experimental Parasitology, 126(2)184-186.

Potenza, M.R., J.D. Felicio, V. Arthur, M.H. Rossi, M. Nakaoka Sakita, D. de F. Silvestre and D.H.P. Gomes. 2004. Efeito de produtos naturais irradiados sobre Sitophilus zeamais Mots. (Coleoptera; Curculionidae). Arquivos do Instituto Biológico, 71(4): 477-484.

Prado, G.P. do, L.M. Stefani, A.S. da Silva, L.F. Smaniotto, F.R.M. Garcia and N.F. de. Moura. 2013. Alphitobius diaperinus (Coleoptera: Tenebrionidae) Susceptibility to Cunila angustifolia Essential Oil. Journal of Medical Entomology, 50(5): 1040-1045.

Procópio, S. de O., J.D. Vendramim, J.I. Ribeiro Júnior, J.B. dos Santos. 2003. Bioatividade de diversos pós de origem vegetal em relação à Sitophilus zeamais Mots. (Coleoptera: Curculionidae). Revista Ciência Agrotécnica, 27(6): 1231 1236.

Raven P.H., R.F. Evert and S.E. Eichhorn. 2001. Biologia Vegetal. 6ªed. Koogan. Rio de Janeiro, Brasil. Ribeiro, V.L.S., J.C. Santos, S.A.L. Bordignon, M.A. Apel, A.T Henriques and G.L. von Poser. 2010. Acaricidal properties of the essential oil from Hesperozygis ringens (Lamiaceae) on the cattle tick Riphicephalus (Boophilus) microplus. Bioresource Technology, 101(7): 2506-2509.

Sánches-Ramos, I. and P. Castañera. 2000. Acaricidal activity of natural monoterpenes on Tyrophagus putrescentiae (Schrank), a mite of stored food. Journal of Stored Product Research, 37(1): 93-101.

Savaris, M., S. Lampert, F.R.M. García, S.M. Sabedot-Bordin and N.F. de Moura. 2012. Atividade Inseticida de Cunila angustifolia sobre adultos de Acanthoscelides obtectus em Laboratório. Ciencia y Tecnología, 5(1): 1-5.

Smaniotto, L.F., N.F. de Moura, R.B. Denardin and F.R.M. Garcia. 2010. Bioatividade da Cabralea canjerana (Meliaceae) no controle de adultos de Acanthoscelides obtectus (Coleoptera, Bruchidae) em laboratório. Biotemas, 23(2): 31-35.

Viegas, C.J. 2003. Terpenos com atividade inseticida: Uma alternativa para o controle químico de insetos. Química Nova, 23(3): 390-400.

Xifreda, C.C. and A.C. Mallo. 2001. Citas nuevas o críticas para La flora argentina III: Cunila angustifolia (Lamiaceae: Mentheae). Darwiniana, 39(1-2): 175-178.


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