Epidemiological investigation of witches’ broom disease in Cola nitida ((Vent.) Schott et Endl) plantations in selected regions of Côte d’Ivoire

Mah Eba, Théodore Kouakou Kouadio, Eric-Olivier Tienebo, Wilfried Junior Yao, Bedel Carlos Adou N'Guessan, Anthelme-Jocelin N’cho, Koutoua Séka, Hortense Atta Diallo, Kouabenan Abo

Abstract


The Cola nitida tree represents a significant source of income for thousands of people. However, this crop is severely threatened by witches’ broom disease. The present study aimed to assess the phytosanitary condition of kola orchards by evaluating key epidemiological parameters across various localities. Specifically, correlations between the phenological stage of the orchards, relative humidity, and disease severity were investigated. In addition, the impact of disease severity on trunk and crown girth was analyzed. The highest prevalence (70.12%) and severity index (78.27%) were recorded in Aboisso. Overall, prevalence rates were relatively consistent across the surveyed localities. Pearson correlation analysis revealed no significant relationship between symptom prevalence and orchard relative humidity (r = 0.044, P = 0.893). However, a strong positive correlation was observed between symptom severity and relative humidity (r = 0.843, P < 0.001). A weak negative correlation was found between orchard age and disease severity (r = –0.280, P = 0.127). No significant correlation was detected between disease severity and kola tree trunk girth (r = 0.025, P = 0.66). Interestingly, trees exhibiting witches’ broom symptoms but lower severity indices had the largest crown sizes (r = –0.287, P < 0.001). Witches’ broom disease was found to be widespread in all surveyed areas, with consistently high severity indices. A comprehensive understanding of the epidemiology of witches’ broom disease is essential for the development of more targeted and effective management strategies.


Keywords


Witches’ broom, Cola nitida, Côte d'Ivoire, Epidemiology, Phytosanitary assessment

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References


Agrios, G.N., 2005. Plant Pathology (5th ed.). Elsevier Academic Press, Amsterdam, pp. 26-27, 398–401.

Alves da Silva, N.J., Menezes Reis, S.P., Diorato, V.S., Rocha, J.S.A., Barbosa, C.S., Ciampi-Guillardi, M., Patrocínio, N.G.R.B., Niella, G.R., Solis, K., Peñaherrera, S., Manco, M.J.D.S., Teixeira, G.A., Arévalo-Gardini, E., Gramacho, K.P., 2022. A molecular diagnostic for Moniliophthora perniciosa, the causal agent of witches’ broom disease of cacao, that differentiates it from its sister taxon Moniliophthora roreri. Crop Protection 158, 105959.

Andrade, B.S., Taranto, A.G., Röessle, S.C.S., Góes-Neto, A., Avery, M.A., 2008. Comparative modeling of DNA and RNA polymerases from Moniliophthora perniciosa mitochondrial plasmid. International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics 2008, BCBGC 2008, 81-86.

Bastos, C.N., Evans, H.C., 2015. Ecology and epidemiology of Moniliophthora perniciosa: implications for sustainable disease management. Plant Pathology 64, 1-12.

Carvajal-Rivera, A.S., Jaimes-Suárez, Y.Y., Guzmán-Plazola, R.A., Ortiz-García, C.F., Sandoval-Islas, J.S., 2022. Temporal dynamics of witches’ broom disease (Moniliophthora perniciosa) in six cocoa clones with and without shading. Journal of Plant Pathology 104, 37-48.

de Oliveira, I.B., Moura, I.M., Santana, J.O., Gramacho, K.P., Santos Alves, S.D., Ferreira, M.M., Santos, A.S., de Novais, D.P.S., Pirovani, C.P., 2024. Cocoa apoplastome contains defense proteins against pathogens. Phytopathology 114, 427-440.

Dumartinet, T., 2021. Étude de l’adaptation aux résistances variétales chez le champignon Pseudocercospora fijiensis, agent causal de la maladie des raies noires du bananier. Thèse de doctorat, Université de Montpellier, 163 p.

Eba, M., Kouadio, K., Séka, K., Yao, W.J., Dosso, G.M., N’Guessan, A.B.C., N’Cho, A.J., Abo, K., 2025. Morphological and molecular diversity of fungal microflora associated with kola tree suffering from witches’ broom disease (Cola sp.) in Côte d'Ivoire. International Journal of Agriculture and Biology 33, 330513.

Ferreira, L.F., Duarte, K.M., Gomes, L.H., Carvalho, R.S., Leal Junior, G.A., Aguiar, M.M., Armas, R.D., Tavares, F.C., 2012. Genetic diversity of polysporic isolates of Moniliophthora perniciosa (Tricholomataceae). Genetics and Molecular Research 11, 2559-2568.

Gbedié, N.A., Kouadio, K.T., Ouattara, Y., Coulibaly, K., Bonsson, B., Sery, D., Legnate, H.N., Kéli, J.Z., 2019. Évaluation de clones de colatiers (Cola nitida [Vent.] Schott et Endlicher) pour leur tolérance à la maladie du balai de sorcière du colatier en Côte d’Ivoire. International Journal of Biological and Chemical Sciences 13, 3347-3354.

Griffith, G.W., Nicholson, J., Nenninger, A., Birch, R.N., Hedger, J.N., 2003. Witches’ brooms and frosty pods: two major pathogens of cacao. New Zealand Journal of Botany 41, 423-435.

Koffi, A., 2015. Pathogénicité et diversité génétique des parasites fongiques des graines et feuilles de Lagenaria siceraria (Molina) Standl (Cucurbitaceae) de cinq localités de Côte d’Ivoire. Thèse de Doctorat, Université Nangui Abrogoua, 184 p.

Lepoivre, P., 2007. Phytopathologie. Bases moléculaires et biologiques des pathosystèmes et fondements des stratégies de lutte. Presses Agronomiques de Gembloux, De Boeck Université, Bruxelles, Belgique, 432 p.

Magarey, R.D., Sutton, T.B., Thayer, C.L., 2005. A simple generic infection model for foliar fungal plant pathogens. Phytopathology 95, 92-100.

Meinhardt, L.W., Rincones, J., Bailey, B.A., Aime, M.C., Griffith, G.W., Zhang, D., Pereira, G.A.G., 2008. Moniliophthora perniciosa, the causal agent of witches’ broom disease of cacao: What’s new from this old foe? Molecular Plant Pathology 9, 57-588.

Mondego, J.M.C., Carazzolle, M.F., Costa, G.G.L., Formighieri, E.F., Parizzi, L.P., Rincones, J., Cotomacci, C., Carraro, D.M., Cunha, A.F., Carrer, H., Vidal, R.O., Estrela, R.C., García, O., Thomazella, D.P.T., de Oliveira, B.V., Pires, A.B.L., Rio, M.C., Araújo, M.R.R., de Moraes, M.H., Castro, L.A.B., Gramacho, K.P., Gonçalves, M.S., Neto, J.P.M., Neto, A.G., Barbosa, L.V., Guiltinan, M.J., Bailey, B.A., Meinhardt, L.W., Cascardo, J.C.M., Pereira, G.A.G., 2008. A genome survey of Moniliophthora perniciosa gives new insights into witches' broom disease of cacao. BMC Genomics 9, 548.

Ouattara, Y., Bonsson, B., Traoré, M., Gbédié, N., Akaffou, D., Sié, R., Keli, Z., 2018. Évaluation de clones de colatiers (Cola nitida [Vent.] Schott et Endlicher) pour leur tolérance à la maladie du balai de sorcière du colatier en Côte d’Ivoire. International Journal of Biological and Chemical Sciences 13, 3347-3354.

Paschoal, D., Cazetta, L., Mendes, J.V.O., Dias, N.C.F., Ometto, V., Carrera, E., Rossi, M.L., Aricetti, J.A., Mieczkowski, P., Carvalho, G.G., Cesarino, I., da Silva, S.F., Ribeiro, R.V., Teixeira, P.J.P.L., da Silva, E.M., Figueira, A., 2025. Root development of tomato plants infected by the cacao pathogen Moniliophthora perniciosa is affected by limited sugar availability. Plant, Cell and Environment 48, 3603-3619.

Pires, A.B.L., Gramacho, K.P., Silva, D.C., Góes-Neto, A., Silva, M.M., Muniz-Sobrinho, J.S., Porto, R.F., Villela-Dias, C., Brendel, M., Cascardo, J.C.M., Pereira, G.A.G., 2009. Early development of Moniliophthora perniciosa basidiomata and developmentally regulated genes. BMC Microbiology 9, 74.

Rempel, C.B., Hall, R., 1996. Comparison of disease measures for assessing resistance in canola (Brassica napus) to blackleg (Leptosphaeria maculans). Canadian Journal of Botany 74, 1930-1936.

Scarpari, L.M., Meinhardt, L.W., Maizzafera, P., Pomella, A.W.V., Schiavinato, M.A., Cascardo, J.C.M., Pereira, G.A.G., 2005. Biochemical changes during the development of witches’ broom: the most important disease of cocoa in Brazil caused by Crinipellis perniciosa. Journal of Experimental Botany 56, 865-877.

Sousa Filho, H.R., de Jesus, R.M., Bezerra, M.A., Santana, G.M., de Santana, R.O., 2021. History, dissemination, and field control strategies of cocoa witches’ broom. Plant Pathology 70, 1971-1978.

Vidhyasekaran, P., 2010. Fungal Pathogenesis in Plants and Crops: Molecular Biology and Host Defense Mechanisms (2nd ed.). CRC Press, 536 p.

Zaparoli, G., Cabrera, O.G., Medrano, F.J., Tiburcio, R., Lacerda, G., Pereira, G.G., 2009. Identification of a second family of genes in Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao, encoding necrosis-inducing proteins similar to cerato-platanins. Mycological Research 113, 61-72.




DOI: https://doi.org/10.33804/pp.009.03.5715

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