Peroxidases of Aspergillus niger for Decolorization of Azo and Anthraquinone and Heterocyclic Dyes

Shazia Erum, Safia Ahmed, Arifa Khan

Abstract


Aspergillus niger SA1, was screened for the production of Peroxidase enzymes (Laccase, Lignin peroxidase “LiP” and Manganese peroxidase i.e., MnP), on agar plates and in liquid cultures. Production media supplemented with AR 151 dye (10 ppm) was the best among all tested media for all the three enzymes (Laccase, LiP and MnP). Maximum production of Laccase was 1784.38 IU/ml by Aspergillus niger SA1. Effect of incubation time for the production of all the three enzymes increased as the fermentation progressed from 24 hr to 168 hrs. Characterization of crude lignin peroxidase for substrate concentration, pH and temperature with the selected fungal strain gave significant rise in the activity of LiP with an elevated concentration of substrate from 2.5 to 10 mm. Optimum pH of 7 was found for LiP activity for the strain. Significant interaction was observed at different temperatures with LiP activity from fungal strain, highest LiP activity (63.32 IU/ml) at 80°C and reduced activity (13.36 IU/ml) at 10°C. Molecular weight of Laccase, Manganese peroxidase and Lignin peroxidase after pretreatment of crude enzyme was found 66.2, 43 and 38Kda, respectively. Peroxidase was purified by column chromatography (Sphadex G-75 and DEAE cellulose column cartridge with a linear gradient of 0.1-0.5 M NaCl in Tris buffer pH 7.5). Over all a coexistence of higher absorbance Vs activity was observed in the elevated peaks with the fungal strain. Maximum fold purification was observed with the greater yield of the enzyme activity originally present in the crude enzyme. It was found that minimal time of 10 sec was sufficient for decreasing the absorbance of initial color for all four textiles dyes (AR 151, DBK2RL, Orange II and Sulphur black) with crude and purified enzyme.

Keywords


Laccase; Textile dyes; Decolorization; LiP; Mnp; Aspergillus; Copper

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References


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DOI: https://doi.org/10.33687/planthealth.04.01.5977

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