Evaluation of Chili Germplasm against Leaf Spot and its Management by Nutrients and Fungicides

Bint e Zainab, Safdar Ali, Muhammad Ahmad Zeshan, Ghulam Mustafa Sahi, Yasir Iftikhar, Rana Binyamin, Muhammad Usman Ghani, Adeel Ahmad


Chili (Capsicum annum L.) is a widely grown vegetable in tropical and subtropical areas that is attacked by a diverse range of bacterial and fungal pathogens, causing severe yield and quality losses. Alternaria leaf spot, caused by Alternaria alternata, is the major fungal disease of chili that deteriorates the vitality of the crop. The objective of the current research is to identify the most effective nutrient combination and fungicide against Alternaria leaf spot disease and to evaluate resistant sources. The current research was conducted using six varieties (CH-121, Chilli-900 F1, Red Wing, SAYBAN, AAHP-1, and Diamond CH-121) collected from Ayub Agricultural Research Institute, Faisalabad, Pakistan. Seeds were sown in the Plant Pathology field area, University of Agriculture, Faisalabad, to screen against leaf spot disease. The effect of different fungicides and nutrients to overcome chili leaf spot was studied, and three treatment combinations were prepared: (ZnSO4 (ZS) + MnSO4 (MS) + boric acid (BA) + Topsin M), (ZS + MS + BA + Mancozeb), and (ZS + MS + BA + Vitavax). Among these, the combination of (ZS + MS + BA + Vitavax) was the most effective against leaf spot disease. Minimum disease severity was observed with ZS + MS + BA + Vitavax in CH-121 (15.00), while maximum disease severity was observed with the control in Red Wing (93.67). Environmental data were collected and statistically analyzed. There was a significant (p < 0.05) but positive correlation between maximum temperature, minimum temperature, wind speed, rainfall, and disease severity. There was a negative correlation between relative humidity and disease severity. In conclusion, micronutrients were found to enhance Alternaria leaf spot tolerance in chili plants, providing a cost-effective and easily obtainable solution. The combined use of nutrients and fungicide is effective in preventing the development of resistance in pathogens against fungicides.


Alternaria alternata; Micronutrients; Screening of seeds; Environmental factors; MnZO4

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AbdElAziz, G.H., El-Rahman, A., Lamyaa, A., Ahmed, S.S., Mahrous, S.E.M., 2021. Efficacy of ZnO nanoparticles as a remedial zinc fertilizer for soya bean and wheat corps. Journal of Soil Sciences and Agricultural Engineering 12, 573-582.

Amrao, L., Ahmed, M.Z., Saeed, S., Shafique, M.S., Perveen, R., Anwaar, H.A., Sheikh, U.A.A, Iqbal, R., Ali, S., Azmat, S., Aslam, M., 2022. First Report of Alternaria alternata causing leaf spot on chili (Capsicum annuum L.) in Pakistan. Plant Disease 106, 1073.

Arin, S., 2019. Scenario of chilli production and hindrances faced by the growers of Sindh province of Pakistan. Modern Concepts and Developments in Agronomy 4, 436-442.

Asghar, A., Mukhtar, T., Raja, M.U., Gulzar, A., 2020. Interaction between Meloidogyne javanica and Ralstonia solanacearum in chili. Pakistan Journal of Zoology 52(4), 1525-1530.

Ashfaq, M., Iqbal, S., Mukhtar, T., Shah, H., 2014. Screening for resistance to cucumber mosaic cucumovirus in chilli pepper. Journal of Animal and Plant Sciences 24(3), 791-795.

Aslam, M.N., Mukhtar T., 2024. Evaluation of virulence among Pakistani isolates of Ralstonia solanacearum inducing bacterial wilt in chilies across different agro-ecological zones. Bragantia 83, e20230181. https://doi.org/10.1590/1678-4499.20230181

Aslam, M.N., Mukhtar, T., 2023a. Characterization of Ralstonia solanacearum causing bacterial wilt from major chili growing areas of Pakistan. Bragantia, 82, e20230001. https://doi.org/10.1590/1678-4499.20230001

Aslam, M.N., Mukhtar, T., 2023b. Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan. Bragantia, 82, e20220196. https://doi.org/10.1590/1678-4499-2022-0196

Aslam, M.N., Mukhtar, T., Ashfaq, M., Hussain, M.A., 2017. Evaluation of chili germplasm for resistance to bacterial wilt caused by Ralstonia solanacearum. Australasian Plant Pathology 46(3), 289-292.

Awan, Z.A., Shoaib, A., Khan, K.A., 2019. Crosstalk of Zn in combination with other fertilizers underpins interactive effects and induces resistance in tomato plant against early blight disease. The Plant Pathology Journal 35, 330.

Cabot, C., Martos, S., Llugany, M., Gallego, B.,Tolrà, R., Poschenrieder, C., 2019. A role for zinc in plant defense against pathogens and herbivores. Frontiers in Plant Science 10, 1171.

Costa, J., Rodríguez, R., Garcia-Cela, E., Medina, A., Magan, N., Lima, N., Battilani, P., Santos, C., 2019. Overview of fungi and mycotoxin contamination in Capsicum pepper and in its derivatives. Toxins 11, 27.

Du, W., Yang, J., Peng, Q., Liang, X., Mao, H., 2019. Comparison study of zinc nanoparticles and zinc sulphate on wheat growth: From toxicity and zinc biofortification. Chemosphere 227, 109-116.

Garibaldi, A., Gilardi, G., Matic, S., Gullino, M.L., 2019. First report of Alternaria alternata on chili pepper (Capsicum frutescens) in Italy. Plant Disease 103, 1024.

Geddes‐McAlister, J., Shapiro, R.S., 2019. New pathogens, new tricks: emerging, drug‐resistant fungal pathogens and future prospects for antifungal therapeutics. Annals of the New York Academy of Sciences 1435, 57-78.

Gomes, M.H.F., de Almeida Machado, B., Marques, J.P.R., Otto, R., Eichert, T., Pereira de Carvalho, H.W., 2020. Foliar application of Zn phosphite and Zn EDTA in soybean (Glycine max (L.) Merrill): in vivo investigations of transport, chemical speciation, and leaf surface changes. Journal of Soil Science and Plant Nutrition 20, 2731-2739.

Hernández-Carrión, M., Hernando, I., Quiles, A., 2014. High hydrostatic pressure treatment as an alternative to pasteurization to maintain bioactive compound content and texture in red sweet pepper. Innovative Food Science and Emerging Technologies 26, 76-85.

Hossain, M.M., Pérez-López, E., Todd, C.D., Wei, Y., Bonham-Smith, P.C., 2021. Endomembrane-targeting Plasmodiophora brassicae effectors modulate PAMP triggered immune responses in plants. Frontiers in Microbiology 1687.

Iqbal, S.Z., Asi, M.R., Mehmood, Z., Mumtaz, A., Malik, N., 2017. Survey of aflatoxins and ochratoxin A in retail market chilies and chili sauce samples. Food Control 81, 218-223.

Iqbal, S.Z., Mumtaz, A., Mahmood, Z., Waqas, M., Ghaffar, A., Ismail, A., Pervaiz, W., 2021. Assessment of aflatoxins and ochratoxin A in chili sauce samples and estimation of dietary intake. Food Control 121, 107621.

Ismail, A.M., Heuer, S., Thomson, M.J., Wissuwa, M., 2007. Genetic and genomic approaches to develop rice germplasm for problem soils. Plant molecular Biology 65, 547-570.

Khruengsai, S., Pripdeevech, P., D'Souza, P.E., Panuwet, P., 2021. Biofumigation activities of volatile compounds from two Trichoderma afroharzianum strains against Fusarium infections in fresh chilies. Journal of the Science of Food and Agriculture 101, 5861-5871.

Kumar, I., Bhattacharya, J., Das, B.K., 2022. Efficacy of silver nanoparticles-based foliar spray application to control plant diseases, its effect on productivity, and risk assessment. Arabian Journal of Geosciences 15, 1-11.

Li, Z., Fan, Y., Gao, L., Cao, X., Ye, J., Li, G., 2016. The dual roles of zinc sulfate in mitigating peach gummosis. Plant Disease 100, 345-351.

Matić, S., Tabone, G., Garibaldi, A., Gullino, M.L., 2020. Alternaria leaf spot caused by Alternaria species: an emerging problem on ornamental plants in Italy. Plant Disease 104, 2275-2287.

Moya-Elizondo, E.A., Lagos, M.J., San Martin, J., Ruiz, B., 2021. First Report of Alternaria alternata and Fusarium spp. causing brown apical necrosis in Walnut fruit in southern Chile. Plant Health Progress 22, 573-574.

Mukhtar, T., Vagelas, I., Javaid, A., 2023. Editorial: New trends in integrated plant disease management. Frontiers in Agronomy 4, 1104122. doi: 10.3389/fagro.2022.11041221

Nguyen, M.H., Tran, T.N.M., Vu, N.B.D., 2021. Antifungal activity of essential oil-encapsulated lipid nanoemulsions formulations against leaf spot disease on tomato caused by Alternaria alternata. Archives of Phytopathology and Plant Protection 55, 235-257.

Parey, M.A., Razdan, V.K., Sofi, T.A., 2013. Comparative study of different fungi associated with fruit rot of chilli and screening of chilli germplasm against Colletotrichum capsici. International Journal of Agriculture and Crop Sciences 5, 723.

Pickel, B., Dai, N., Maymon, M., Elazar, M., Tanami, Z., Frenkel, O., Toamy, M.A., Mor, N., Freeman, S., 2020. Development of a reliable screening technique for determining tolerance to Macrophomina phaseolina in strawberry. European Journal of Plant Pathology 157, 707-718.

Prasad, Y., Naik, M.K., 2003. Evaluation of genotypes, fungicides and plant extracts against early blight of tomato caused by Alternaria solani. Indian Journal of plant protection 31, 49-53.

Rais, M.U.N., Mangan, T., Sahito, J.G.M., Qureshi, N.A., 2021. A trend analysis: Forecasting growth performance of production and export of chilli in Pakistan. Sarhad Journal of Agriculture 37, 220-225.

Rehman, R., Asif, M., Cakmak, I., Ozturk, L., 2021. Differences in uptake and translocation of foliar‐applied Zn in maize and wheat. Plant and Soil 462, 235-244.

Roggero, P., Dellavalle, G., Ciuffo, M., Pennazio, S., 1999. Effects of temperature on infection in Capsicum sp. and Nicotiana benthamiana by impatiens necrotic spot tospovirus. European Journal of Plant Pathology 105, 509-512.

Saba, S., Mukhtar, T., Haq, M.I., Malik, S.I., 2022. Occurrence of damping off of chili caused by Pythium spp. in the Pothwar region of Pakistan. International Journal of Phytopathology 11(2), 125-234.

Sarada, C., Naidu, L., Ramana, C.V., 2015. Effect of weather factors on chilli production and strategies for improvement of productivity. Journal of Progressive Agriculture 6, 5-9.

Sarkar, A., Chakraborty, N., Acharya, K., 2021. Unraveling the role of nitric oxide in regulation of defense responses in chilli against Alternaria leaf spot disease. Physiological and Molecular Plant Pathology 114, 101621.

Sarkar, A., Chakraborty, N., Acharya, K., 2022. Chitosan nanoparticles mitigate Alternaria leaf spot disease of chilli in nitric oxide dependent way. Plant Physiology and Biochemistry 180, 64-73.

Savi, G.D., Bortoluzzi, A.J., Scussel, V.M., 2013. Antifungal properties of Zinc‐compounds against toxigenic fungi and mycotoxin. International Journal of Food Science and Technology 48, 1834-1840.

Shahbaz, M., Akram, A., Raja, N.I., Mukhtar, T., Mehak, A., Fatima, N., Ajmal, M., Ali, K., Mustafa, N., Abasi, F. 2023. Antifungal activity of green synthesized selenium nanoparticles and their effect on physiological, biochemical, and antioxidant defense system of mango under mango malformation disease. PLoS ONE 18(2): e0274679. https://doi.org/10.1371/journal.pone.0274679

Shahbaz, M.U., Mukhtar, T., Haque, M.I., Begum, N., 2015. Biochemical and serological characterization of Ralstonia solanacearum associated with chilli seeds from Pakistan. International Journal of Agriculture and Biology 17 (1), 31-40.

Shoaib, A., Akhtar, M., Javaid, A., Ali, H., Nisar, Z., Javed, S., 2021. Antifungal potential of zinc against leaf spot disease in chili pepper caused by Alternaria alternata. Physiology and Molecular Biology of Plants 27, 1361-1376.

Shoaib, A., Ali, H., Javaid, A., Awan, Z.A., 2020. Contending charcoal rot disease of mungbean by employing biocontrol Ochrobactrum ciceri and zinc. Physiology and Molecular Biology of Plants 26, 1385-1397.

Shoaib, A., Awan, Z.A., 2021. Mineral fertilizers improve defense related responses and reduce early blight disease in tomato (Solanum lycopersicum L.). Journal of Plant Pathology 103, 217-229.

Silva, A.C.D., Resende, M.L.V.D., Souza, P.E.D., Pôssa., K.F., Silva Júnior, M.B.D., 2016. Plant extract, zinc phosphite and zinc sulphate in the control of powdery mildew in the eucalyptus. Revista Ciência Agronômica 47, 93-100.

Singh, H., Kaur, S., Singh, J., 1993. Determination of infection in fruit rot (Colletotrichum capsici) of chilli (Capsicum annuum L.). Indian Journal of Agricultural Sciences 63, 310-312.

Soomro, H.U., Khaskheli, M.I., Hyder, M., Khan, R.A.A., Bukero, A., Panhwar, S., Bukero, A.A., Larik, A.Q., 2019. Disease intensity and eco-friendly management of Alternaria alternata in chili (Capsicum annuum L.). Pure and Applied Biology 8, 2333-2342.

Tariq-Khan, M., Mukhtar, T., Munir, A., Hallmann, J., Heuer, H., 2020. Comprehensive report on the prevalence of root-knot nematodes in the Poonch division of Azad Jammu and Kashmir, Pakistan. Journal of Phytopathology 168, 322-336.

Tariq-Khan, M., Munir, A., Mukhtar, T., Hallmann, J., Heuer, H., 2017. Distribution of root-knot nematode species and their virulence on vegetables in northern temperate agro-ecosystems of the Pakistani-administered territories of Azad Jammu and Kashmir. Journal of Plant Diseases and Protection 124(3), 201-212.

Thakur, H., Jindal, S.K., Sharma, A., Dhaliwal, M.S., 2018. Chilli leaf curl virus disease: a serious threat for chilli cultivation. Journal of Plant Diseases and Protection 125, 239-249.

Yadav, M.K., Tyagi, S., Javeria, S., Gangwar, R.K., Singh, R., 2015. Evaluation of the efficacy of different fungicide for the management of Alternaria Leaf spot disease of chilli. European Journal of Medicine 1, 53-55.

Zeeshan, M., Mukhtar, T., Haq, M.I., Asad, M.J. 2023. Incidence, characterization and pathogenic variability of Fusarium oxysporum in the Punjab province of Pakistan. International Journal of Phytopathology 12(1), 1-11.

DOI: https://doi.org/10.33804/pp.007.03.4862


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