What Makes Research on Aflatoxin Crucial for Human Health and Development?

Amir Afzal, Sharmin Ashraf, Sairah Syed, Ruqeah Mustafa, Madeeha Khan, Javed Iqbal, Uzma Javed, Qamar Shakil


Food security involves not only ensuring access to adequate food, but also ensuring that the food is nutritious and free from contaminants that can harm human health. Aflatoxins, produced by the fungi Aspergillus flavus and A. parasiticus, are one example of a contaminant that can pose a threat to food security. To address this issue, it is important to implement effective management practices throughout the food chain, from the field to the table, to minimize the risk of aflatoxin contamination. Additionally, promoting awareness about the dangers of aflatoxins at the social level and using a combination of genetic and management practices can help provide a stable and sustainable solution to this problem. This article extensively discusses the harmful effects of Aflatoxins (AFs) and outlines strategies for managing contamination before and after harvest, including the potential for breeding crops with higher resistance. The article also examines the relationship between moisture content and AF contamination in peanuts before harvest. It highlights the impact of AFs on the production of meat, milk, and eggs from animals and underscores the need for a comprehensive policy to prevent AF contamination from entering the food chain at every stage. Improved agronomic practices and cattle feeding, as well as public awareness efforts, can help reduce the risk of AFs in the food supply. With a combination of current genetic improvements and effective pre- and post-harvest management practices, it may be possible to mitigate the issue of AF contamination


Aflatoxin; AFs; Aflatoxin contamination; Aspergillus spp.; Aspergillus flavus; Aspergillus parasiticus

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Abbas, H.K., 2002. Aflatoxin and fumonisin contamination of commercial corn (Zea mays) hybrids in Mississippi. Journal of Agriculture Food Chemistry 50, 5246-5254.

Acur, A., Arias, R.S., Odongo, S., Tuhaise, S., Sekandi, J., Adriko, J., Muhanguzi D., Buah, S., Kiggundu, A., 2020. Genetic diversity of AF-Producing Aspergillus flavus isolated from selected groundnut growing agro-ecological zones of Uganda. BMC Microbiology 20, 1-12.

AEFS, 2013. Aflatoxins (sum of B1, B2, G1, G2) in cereals and cereal-derived food products. Efsa Supporting Publications 10, n/a-n/a.

Ahmed, R., Riaz, A., Zakria, M., Naz, F., 2013. Incidence of karnal bunt (Tilletia indica Mitra) of wheat (Triticum aestivum L.) in two districts of Punjab (Pakistan) and identification of resistance source. Pakistan Journal of Phytopathology 25, 01-06.

Alam, M., Rehman, A., Gleason, M., Khan, A., Amin, M., Ali, S., Fiaz, M., Ahmed, R., 2017. First report of Alternaria alternata causing postharvest fruit rot of lychee in Pakistan. Plant Disease 101, 1041.

Amaike, S., Keller, N.P., 2011. Aspergillus flavus. Annual Review of Phytopathology 49, 107-133.

Anderson, W.F., Holbrook, C.C., Wilson, D.M., Matheron, M.E., 1995. Evaluation of preharvest aflatoxin contamination in several potentially resistant peanut genotypes. Peanut Science, 22, 29-32.

Bairagi, M.D., David, A.A., Thomas, T., Gurjar, P.C., 2017. Effect of different level of N P K and gypsum on soil properties and yield of groundnut (Arachis hypogaea L.) var Jyoti. International Journal of Current Microbiology and Applied Sciences 6, 984-991.

Benkerroum, N., 2020. Chronic and acute toxicities of aflatoxins: mechanisms of action. International Journal of Environmental Research 17, 423.

Bennett, J.W., Klich, M., 2003. Mycotoxins. Clinical Microbiology Reviews 16, 497-516.

Bhatnagar, D., Cleveland, T.E., Cotty, P.J., 1994. Mycological aspects of aflatoxin formation. In: Eaton, D.L. and Groopman, J.D. (eds.) The Toxicology of Aflatoxins. Academic Press, San Diego, California, 327-345.

Boutrif, E., 1998. Prevention of AF in pistachios. Agriculture, Food and Nutrition 21, 32-38.

Brown, L.R., 2018. Aflatoxins in food and feed: Impacts risks, and management strategies. International Food Policy Research Institute, 9.

Cotty, P.J., 1989. Virulence and cultural characteristics of two Aspergillus flavus strains pathogenic on cotton. Phytopathology 79, 808-814.

Cotty, P.J., 1997. Aflatoxin-producing potential of communities of Aspergillus section flavi from cotton producing areas in the United States. Mycological Research 101, 698-704.

Cotty, P.J., Antilla, L., Wakelyn, P.J., 2007. Competitive exclusion of aflatoxin producers: Farmer-driven research and development. Biological control: a Global Perspective, 241-253.

Cotty, P.J., Jaime-Garcia, R., 2007. Influences of climate on aflatoxin producing fungi and aflatoxin contamination. International Journal of Food Microbiology 119, 109-115.

D’Mello, J.P.F., Macdonald, A.M.C., 1997. Mycotoxins. Animal Feed Science and Technology 69, 155-166.

Dorner, J.W., 2004. Biological control of aflatoxin contamination of crops. Journal of Toxicology: Toxin Reviews 23, 425-450.

Ehrlich, K. C., 2014. Non-aflatoxigenic Aspergillus flavus to prevent aflatoxin contamination in crops advantages and limitations. Frontiers in Microbiology 5, 50.

Fratamico, P.M., Bhunia, A.K.,Smith J.L., 2008. Foodborne Pathogens: Microbiology and Molecular Biology. Caister Academic Press, Norwich, England, pp. 453.

Gebreselassie, R., Dereje, A., Solomon, H., 2014. On farm pre harvest agronomic management practices of Aspergillus infection on groundnut in Abergelle, Tigray. Journal of Plant Pathology and Microbiology 5, 1- 6.

Ghuffar, S., Irshad, G., Zhai, F., Aziz, A., Asadullah, H.M.A.M., Mehmood, N., Yang, H., Bashir, A., Ahmed, M.Z., Aslam, M.F., 2018. First report of Fusarium proliferatum causing fruit rot of grapes (Vitis vinifera) in Pakistan. International Journal of Phytopathology 7, 85-88.

Gong, Y.Y., Watson, S., Routledge, M.N., 2016. Aflatoxin exposure and associated human health effects, a review of epidemiological studies. Food Safety 4, 14-27.

Goto, T., Wicklow, D.T., Ito, Y., 1996. Aflatoxin and cyclopiazonic acid production by a sclerotium-producing Aspergillus tamarii strain. Applied and Environmental Microbiology 62, 4036-4038.

Hamidou, S.K., Kadidia, K., Alassane, O., Mohamed, S., Itolou, K.A., Harouna, S., Claudine, C., 2022. Pathogenic characterization of three Fusarium species associated with onion (Allium cepa L.) in Burkina Faso. International Journal of Phytopathology 11, 267-276.

Hesseltine, C.W., 1974. Natural occurrence of mycotoxins in cereals. Mycopathologia et Mycologia applicata 53, 141-153.

Holbrook, C.C., Guo, B.Z., Wilson, D.M., Timper, P., 2009. The US breeding program to develop peanut with drought tolerance and reduced aflatoxin contamination. Peanut Science 36, 50-53.

Horn, B.W., Dorner, J.W., 1998. Soil populations of Aspergillus species from section Flavi along a transect through peanut-growing regions of the United States. Mycologia 90, 767-776.

Horn, B.W., Greene, R.L., Dorner, J.W., 1995. Effect of corn and peanut cultivation on soil populations of Aspergillus flavus and A. parasiticus in southwestern Georgia. Applied and Environmental Microbiology 61, 2472-2475.

Horn, B.W., Ramirez-Prado, J.H., Carbone, I., 2009. The sexual state of Aspergillus parasiticus. Mycologia, 101, 275-280.

Hua, S.S.T., Baker, J.L., Flores-Espiritu, M., 1999. Interactions of saprophytic yeasts with a nor mutant of Aspergillus flavus. Applied and Environmental Microbiology 65, 2738-2740.

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, 2012. Chemical agents and related occupations. IARC monographs on the evaluation of carcinogenic risks to humans, 100(PT F), p.9.

ICRISAT. 2016. How to Reduce Aflatoxin Contamination in Groundnuts and Maize A Guide for Extension Workers. Patancheru 502 324, Telangana, India: International Crops Research Institute for the Semi-Arid Tropics. 24 pp.

Ijaz, M., Afzal, A., Ali, S.R., Nawaz, H.H., Altaf, R., Jannat, F.T., Syed,S. 2022. Aflatoxin: Can be managed by soil amendments in groundnut? Pakistan Journal Phytopathology 34(01):65-70.

Iqbal, S.Z., Jinap, S., Pirouz, A.A., Faizal, A.A., 2015. Aflatoxin M1 in milk and dairy products, occurrence and recent challenges: A review. Trends in Food Science and Technology 46, 110-119.

Iqbal, S.Z., Nisar, S., Asi, M.R., Jinap, S., 2014. Natural incidence of aflatoxins, ochratoxin A and zearalenone in chicken meat and eggs. Food Control 43, 98-103.

Ismaiel, A.A., Papenbrock, J., 2015. Mycotoxins: producing fungi and mechanisms of phytotoxicity. Agriculture 5, 492-537.

Jaimez, J., Fente, C.A., Vazquez, B.I., Franco, C.M., Cepeda, A., Mahuzier, G., Prognon, P., 2000. Application of the assay of aflatoxins by liquid chromatography with fluorescence detection in food analysis. Journal of Chromatography A 882, 1-10.

Jain, M., Pathak, B.P., Harmon, A.C., Tillman, B.L., Gallo, M., 2011. Calcium dependent protein kinase (CDPK) expression during fruit development in cultivated peanut (Arachis hypogaea) under Ca2+ sufficient and deficient growth regimens. Journal of Plant Physiology 168, 2272-2277.

Jeyaramraja, P.R., Meenakshi, S.N., Woldesenbet, F., 2018. Relationship between drought and preharvest aflatoxin contamination in groundnut (Arachis hypogaea L.). World Mycotoxin Journal 11,187-199.

Kamle, M., Mahato, D.K., Devi, S., Lee, K.E., Kang, S.G., Kumar, P., 2019. Fumonisins: Impact on agriculture, food, and human health and their management strategies. Toxins 11, 328.

Kensler, T.W., Roebuck, B.D., Wogan, G.N., Groopman, J.D., 2011. Aflatoxin: a 50-year odyssey of mechanistic and translational toxicology. Toxicological Sciences 120, S28-S48.

Kew, M.C., 2013. Aflatoxins as a cause of hepatocellular carcinoma. Journal of Gastrointestinal and Liver Diseases 22, 305-310.

Klich, M.A., 2007. Aspergillus flavus: the major producer of aflatoxin. Molecular Plant Pathology 8, 713-722.

Kumar, A., Pathak, H., Bhadauria, S., Sudan, J., 2021. Aflatoxin contamination in food crops: causes, detection, and management: a review. Food Production, Processing and Nutrition 3, 1-9.

Kumar, P., Mahato, D.K., Kamle, M., Mohanta, T.K., Kang, S.G., 2017. Aflatoxins: a global concern for food safety, human health and their management. Frontiers in microbiology 7, 2170.

Lalah, J.O., Omwoma, S., Orony, D.A., 2020. Aflatoxin B1 Chemistry environmental and diet sources and potential exposure in human in Kenya. In Aflatoxin B1 Occurrence Detection and Toxicological Effects: Intech Open.

Liang, X.A., Luo, M., Guo, B.Z., 2006. Resistance mechanisms to Aspergillus falvus infectin and aflatoxin contaminatin in peanut (Arachis hypogaea). Plant Pathology Journal 5,115-124.

Masoud, W., Kaltoft, C.H., 2006. The effects of yeasts involved in the fermentation of Coffea arabica in East Africa on growth and ochratoxin A (OTA) production by Aspergillus ochraceus. International Journal of Food Microbiology 106, 229-234.

Massomo, S.M., 2020. Aspergillus flavus and aflatoxin contamination in the maize value chain and what needs to be done in Tanzania. Scientific African 10, p.e00606.

McDonald, D., Mehan, V.K., (Eds), Aflatoxin contamination of groundnuts: Proceedings of the International Workshop, 6–9 OCT 1987, ICRISAT Center, India. Patancheru, AP 502 324, India: ICRISAT pp. 269-278

Medina, A., Gilbert, M.K., Mack, B.M., OBrian, G.R., Rodriguez, A., Bhatnagar, D., Payne, G., Magan, N., 2017. Interactions between water activity and temperature on the Aspergillus flavus transcriptome and aflatoxin B1 production. International Journal of Food Microbiology 256, 36-44.

Mehan, V.K., 1989. Screening groundnuts for resistance to seed invasion by Aspergillus flavus and to aflatoxin production. pp. 323-334 in: Aflatoxin contamination of groundnut; Proceeding of International Workshop 6 - 9 Oct 1987, ICRISAT Center, India (McDonald, D., and Mehan, V.K., eds) Patancheru 502 324 Andhra Pradesh India: International Crops Research Institute for the Semi-Arid Tropics.

Mehan, V.K., McDonald, D., Haravu, L.J., Jayanthi, S., 1991. The groundnut aflatoxin problem review and literature database. International Crops Research Institute for the Semi-Arid Tropics. Patancheru, AP 502 324, India ICRISAT.

Mixon, A.C., Rogers, K.M., 1973. Peanut accessions resistant to seed infection by Aspergillus flavus 1. Agronomy Journal 65, 560-562.

Mohsenzadeh, M.S., Hedayati, N., Riahi-Zanjani, B., Karimi, G., 2016. Immunosuppression following dietary aflatoxin B1 exposure: a review of the existing evidence. Toxin Reviews 35,121-127.

Mutegi, C.K., Ngugi, H.K., Hendriks, S.L., Jones, R.B., 2009. Prevalence and factors associated with aflatoxin contamination of peanuts from Western Kenya. International Journal of Food Microbiology 130, 27-34.

Nayak, S.N., Agarwal, G., Pandey, M.K., Sudini, H.K., Jayale, A.S., Purohit, S., Desai, A., Wan, L., Guo, B., Liao, B.,Varshney, R.K., 2017. Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in vitro seed colonization. Scientific Reports 7, 9659.

Nigam, S.N., Waliyar, F., Aruna, R., Reddy, S.V., Kumar, P.L., Craufurd, P.Q., Diallo, A.T., Ntare, B.R., Upadhyaya, H.D., 2009. Breeding peanut for resistance to aflatoxin contamination at ICRISAT. Peanut Science 36, 42-49.

Pandey, A.K., Singh, P., Palni, U.T., Tripathi, N.N., 2014. Effect of variable physical factors on the toxicity of plant volatiles against some Aspergillus spp. International Journal of Phytopathology 3, 63-66.

Pandey, M.K., Kumar, R., Pandey, A.K., Soni, P., Gangurde, S.S., Sudini, H.K., Fountain, J.C., Liao, B., Desmae, H., Okori, P., Chen, X., Jiang H., Mendu V., Falalou H., Njoroge S., Mwololo J., Guo B., Zhuang W., Wang X., Liang X., Varshey R.K., 2019. Mitigating aflatoxin contamination in groundnut through a combination of genetic resistance and post-harvest management practices. Toxins 11, 315.

Pandey, M.K., Roorkiwal, M., Singh, V.K., Ramalingam, A., Kudapa, H., Thudi, M., Chitikineni, A., Rathore, A.,Varshney, R.K., 2016. Emerging genomic tools for legume breeding: current status and future prospects. Frontiers in Plant Science 7, 455.

Payne, G.A., Brown, M.P., 1998. Genetics and physiology of aflatoxin biosynthesis. Annual Review of Phytopathology 36, 329-362

Perrone, G., Haidukowski, M., Stea, G., Epifani, F., Bandyopadhyay, R., Leslie, J.F., Logrieco, A., 2014. Population structure and Aflatoxin production by Aspergillus Sect. Flavi from maize in Nigeria and Ghana. Food Microbiology 41, 52-59.

Pitt J. I. 2000. Toxigenic fungi and mycotoxins. British Medical Bulletin. 56, 184–192.

Quadri, S. H., Ms, N., Kc, C., Shantaram, U., Hs, E. 2012. An overview of chemistry, toxicity, analysis, and control of aflatoxins. International Journal of Chemical and Life Sciences, 2, 1071–1078.

Rajarajan, P.N., Rajasekaran, K.M., Devi, N.K., 2013. AF contamination in agricultural commodities. Indian Journal of Pharmaceutical and Biological Research 1, 148-151.

Rao, K.S., Tulpule, P.G., 1967. Varietal differences of groundnut in the production of aflatoxin. Nature 13;214, 738-9. doi: 10.1038/214738b0. PMID: 6049097.

Rao, M.V., Nigam, S.N., Mehan, V.K., McDonald, D., 1989. Aspergillus flavus resistance breeding in groundnut: progress made at ICRISAT Center. 738-739

Rao, M.V., Nigam, S.N., Mehan, V.K., McDonald, D., 1989. Aspergillus flavus resistance breeding in groundnut: Progress made at ICRISAT Center pp. 345-355.

Rayfuse, R., and Weisfelt, N., 2012. The Challenge of Food Security: International Policy and Regulatory Frameworks. The University of New South Wales. Edward Elgar. Cheltenhum UK. Northampton MA.USA.

Shakoor, S., Inam-ul-Haq, M., Bibi, S., Ahmed, R., 2015. Influence of root inoculations with vasicular arbuscular mycorrhizae and rhizomyx for the management of root rot of chickpea. Pakistan Journal of Phytopathology 27, 153-158.

Shavkiev, J., Kholmamatovich, K.H., Ismoilovna, T.B., Azimova, N., Khodjakbarovich, N.M., Muminovna, K.K., 2022. Some volatile metabolites produced by the antifungal-Trichoderma Asperellum UZ-A4 micromycete. International Journal of Phytopathology 11, 239-251.

Shuping, D.S.S., Eloff, J.N., 2017. The use of plants to protect plants and food against fungal pathogens-A review. African Journal of Traditional, Complementary and Alternative Medicines 14, 120–127.

Thornton, P.K., Jones, P.G., Ericksen, P.J., Challinor, A.J. 2011. Agriculture and food systems in sub-Saharan Africa in a four-plus degree world. Philosophical Transactions of the Royal Society Series A, 369, 117-136.

Torres, A.M., Barros, G.G., Palacios, S.A., Chulze, S.N., Battilani, P., 2014. Review on pre- and post-harvest management of groundnuts to minimize aflatoxin contamination. Food Research International 62, 11-19.

Undumkon, P., Wiredu, A.N., Nagle, M., Bandyopadhyay, R., Muller, J., Vanlauwe, B., 2017. Mycotoxin in Sub-Saharan Africa: Present situation, socio economic impact, awareness and outlook. Food Control 72, 110-122.

Upadhyaya, H.D., Nigam, S.N., Mehan, V.K., Lenne, J.M., 1997. Aflatoxin Contamination Problems in Groundnut in Asia: Aflatoxin contamination of groundnut-prospects of a genetic solution through conventional breeding. Proceedings of the First Working Group Meeting 27–29 May 1996. Ministry of Agriculture and Rural Development Hanoi Vietnam. Patancheru 502 324, Andhra Pradesh India: ICRISAT. 81-85.

Upadhyaya, H.D., Nigam, S.N., Thakur, R.P., 2002. Genetic enhancement for resistance to aflatoxin contamination in groundnut. Proceedings of the Seventh ICRISAT Regional Groundnut Meeting for Western and Central Africa, Cotonu, Benin, 6–8 December 2000. International Crops Research Institute for the Semi-Arid Tropics; Patancheru, India. 29–36.

Utomo, S.D., Anderson, W.F., Wynne, J.C., Beute, M.K., Hagler, J.R., W.M., Payne, G.A., 1990. Estimates of heritability and correlation among three mechanisms of resistance to Aspergillus parasiticus in groundnut. Proceedings of the American Peanut Research and Education Society. Patancheru 502-324 Andhra Pradesh India: ICRISAT. 22:26.

Wagacha, J.M., Muthomi, J.W., 2008. Mycotoxin problem in Africa: Current status implications to food safety and health and possible management strategies. International Journal of Food Microbiology 124, 1-12.

Waliyar, F., Kumar, K.V.K., Diallo, M., Traore, A., Mangala, U.N., Upadhyaya H.D., Sudini, H., 2016. Resistance to preharvest aflatoxin contamination in ICRISAT’s groundnut mini core collection. European Journal of Plant Pathology 145, 901-913.

Waliyar, F., Osiru, M., Ntare, B.R., Kumar, K.V.K., Sudini, H., Traore, A., Diarr, B., 2015. Post-harvest management of aflatoxin contamination in groundnut. World Mycotoxin Journal 8, 245-252.

Waliyar, F., Osiru, M., Sudini, H.K., Njoroge, S.M.C., 2013. Aflatoxins: finding solutions for improved food safety-reducing aflatoxins in groundnuts through integrated management and biocontrol. International Food Policy Research Institute 2033 K Street NW Washington DC 20006-1002 USA.

Wild, C.P., Hall, A.J., 2000. Primary prevention of hepatocellular carcinoma in developing countries. Mutation Research 462, 381-93.

Williams, J.H., Phillips, T.D., Jolly, P.E., Stiles, J.K., Jolly, C.M., Aggarwal, D., 2004. Human aflatoxicosis in developing countries: a review of toxicology exposure potential health consequences and interventions. American Journal of Clinical Nutrition 80, 1106-1122.

World Health Organization. Conference of the Parties to the WHO Framework Convention on Tobacco Control. 1-6 October 2018, Geneva.

Wu, F., Khlangwiset, P., 2010. Health economic impacts and cost-effectiveness of aflatoxin-reduction strategies in Africa: case studies in biocontrol and post-harvest interventions. Food Additives and Contaminants 27, 496-509.

Xue, H., 2004. Evaluation of peanut (Arachis hypogaea L.) germplasm for resistance to aflatoxin production by Aspergillus flavus Link ex Fries. Ph.D Thesis North Carolina State University.

Yan, L., Song, W., Chen, Y., Kang, Y., Lei, Y., Huai, D., Wang, Z., Wang, X., Liao, B., 2021. Effect of non-aflatoxigenic strains of Aspergillus flavus on aflatoxin contamination of pre-harvest groundnuts in fields in China. Oil Crop Science 6, 81-86.

Yin, Y.N., Yan, L.Y., Jiang, J.H., Ma, Z.H., 2008. Biological control of aflatoxin contamination of crops. The Journal of Zhejiang University Science B: Biomedicine and Biotechnology 9, 787-792.

DOI: https://doi.org/10.33804/pp.007.01.4497


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