Combined Efficacy of Aliphatic Hydrocarbons with Neonicotenoids against Phenacoccus solenopsis (Hemiptera: Pseudococcidae) under Controlled Conditions

Mubashar Iqbal, Shahbaz Ahmad, Muhammad Dildar Gogi


Phenacoccus solenopsis appeared as a major pest in recent years and caused huge losses to cotton. Control of cotton mealybug is difficult because of the waxy covering on the body which prevents penetration of insecticides in nymph and adult stages. Present study was carried out under laboratory conditions to evaluate the synergistic effects of minerals oil (Diver®) on the toxicity of diafenthiuron, nitenpyram and thiacloprid against different instars of cotton mealybug. Diafenthiuron, nitenpyram and thiacloprid alone demonstrated mortality in the range of 10.3-46.0%, 13.3-50% and 10.30-46.70% in 1st instar, 8.00-42%, 10.30-44% and 6.60-42.3% in 2nd instar while 7-38%, 7.30-38% and 3.3-32% in adult female of P. solenopsis, respectively. Admixing of 500 ml of Diver® with diafenthiuron administered 65.2-332.7% and 114.6-602.8%; 78.0-428.8% and 128.6-750.0%; and 94.6-457.1% and 147.3-852.9% increase in mortality of 1st instar, 2nd instar and adult female stages of P. solenopsis, respectively. Similarly, fraternization of 1000 ml of Diver® with diafenthiuron administered 42.0-202.6% and 100.0-490.2%; 60.6-261.4% and 127.3-600.2% and 79.8-336.6% and 154.4-854.9% increase in mortality of 1st instar, 2nd instar and adult female stages of P. solenopsis respectively. However, thiacloprid, when admixed with 500 ml and 1000 ml of Diver®, explained 16.7-114.3% and 114.3-633.3%; 24.9-103.6% and 132.3-900.9%; and 0.0-303.9% and 191.7-1990.9% increase in mortality of 1st instar, 2nd instar and adult female stages of P. solenopsis, respectively. In conclusion, Diver® can be exploited as synergist for resistance management of insecticides against P. solenopsis.


Mineral Oil; Neonecotinoid insecticides; P. solenopsis; Toxicity; Mortality

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Abbas, G., Arif, M.J., Ahmad, I., 2007b. Kapas ki mealybug keliey Ihtiati tadabeer wa Iqdamat. (The precautionary measures and management strategies for cotton mealybug) (In Urdu.). Express, p. 15.

Abbas, G., Arif, M.J., Saeed, S., 2005. Systematic status of a new species of the genus Phenacoccus Cockerell (Pseudococcidae), a serious pest of cotton, Gossypium hirsutum L. Pakistan. Pakistan Entomologist 27, 83-84.

Abbas, G., Arif, M.J., Saeed, S., 2006. Identification of mealybug: a novel pest of cotton in Pakistan, 34th SAARC Science Conference, The University of Veterinary and Animal Sciences, Lahore, Pakistan.

Abbas, G., Arif, M.J., Saeed, S., Karar, H., 2007a. Increasing menace of a new mealybug Phenacoccus gossypiphilous to the economic crops of southern Asia, Abstract XI Int. Symposium on Scale Insect Studies (ISSIS), Oeiras.

Ahmad, N., Fatima, B., Khan, G.Z., Nasrullah, Saleem, A., 2003. Field management of insect pests of cotton through augmentation of parasitoid and predators. Asian Journal of Plant Sciences 2, 563-565.

Ahmed, R., Yousaf, J., Saleem, M., Niaz, T., 2012. Efficacy of mineral oil in combination with lower doses of insecticides against cotton mealybug, Phenacoccus solenopsis. Journal of Agricultural Research 50, 103-108.

Anonymous, 2007. Agricultural Statistics of Pakistan, Government of Pakistan. Ministry of Food Agriculture and Livestock, Economic Wing Finance Division, Islamabad Pakistan

Arif, M.I., Rafiq, M., Wazir, S., Mehmood, N., Ghaffar, A., 2012. Studies on cotton mealybug, Phenacoccus solenopsis (Pseudococcidae: Homoptera), and its natural enemies in Punjab, Pakistan. International Journal of Agriculture and Biology 14, 557-562.

Arif, M.J., Gogi, M.D., Abid, A.M., Muhammad, I., Shahid, M.R., Sabir, H., Muhammad, A., 2011. Predatory potential of some native coccinellid predators against Phenacoccus solenopsis, Tinsely (Pseudococcidae: Hemiptera). Pakistan Entomologist 33, 97-103.

Atwal, A., Dhaliwal, G., 2002. Agricultural Pest of South–East Asia & Their Management. Kalyani Publisher, New Delhi, p. 321.

Ben-Dov, Y., Miller, D.R., Gibson, G.A.P., 2009. ScaleNet: A Searchable Information System on Scale Insects. Available on-line at

Bhati, M., Saeed, M., Murtaza, M.A., 1993. Host plant resistance for major cotton bollworms. Pakistan Cotton 37, 1-14.

CCRI, 2006. Annual report of Central Cotton Research Institute (CCRI), Multan for the year 2005-06, p. 23.

Daane, K., Bentley, W., Walton, V., Malakar-Kuenen, R., Millar, J., Ingels, C., Weber, E., Gispert, C., 2006. New controls investigated for vine mealybug. California Agriculture 60, 31-38.

Danho, M., Gaspar, C., Haubruge, E., 2002. The impact of grain quantity on the biology of Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae): oviposition, distribution of eggs, adult emergence, body weight and sex ratio. Journal of Stored Products Research 38, 259-266.

Dhawan, A.K., Sing, J., Sidhu, A.S., 1980. Maconellicoccus sp. attacking Arboreum cotton in Punjab. Journal of Scientific Culture 46, 258.

Finney, D.J., 1971. Probit analysis, Cambridge University Press. Cambridge, UK.

Gogi, M.D., Nauman, I., Arif, M.J., Arshad, M., Bashir, M.H., Khan, M.A., Muhammad, I., 2011. Impact of synergistism with combinations of silicon oil, clove oil and sulfuric acid on the toxicity of some insecticides against cotton mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae). Pakistan Entomologist 33, 137-141.

Henderson, C.F., Tilton, E.W., 1955. Tests with acaricides against the brown wheat mite. Journal of Econmic Entomology 48, 157-161.

Hodgson, C., Abbas, G., Arif, M.J., Saeed, S., Karar, H., 2008. Phenacoccus solenopsis Tinsley (Sternorrhyncha: Coccoidea: Pseudococcidae), an invasive mealybug damaging cotton in Pakistan and India, with a discussion on seasonal morphological variation. Zootaxa 1913, 1–35.

Huque, H., 1972. Cotton Entomology, Cotton in Pakistan PCCC, Karachi, Pakistan, pp. 183-238.

Iftikhar, A., Aziz, M.A., Naeem, M., Ahmad, M., Mukhtar, T., 2018. Effect of temperature on demography and predation rate of Menochilus sexmaculatus (Coleoptera: Coccinellidae) reared on Phenacoccus solenopsis (Hemiptera: Pseudococcidae). Pakistan Journal of Zoology 50, 1885-1893.

JainHua, M.O., 2003. Longtailed mealybug Monog, p: 3. Series-NSW Agriculture, No. 2.

Joshi, M.D., Butani, P.G., Patel, V.N., Jeyakumar, P., 2010. Cotton mealy bug, Phenacoccus solenopsis. Agricultural Reviews 31, 113-119.

Kakakhel, I., 2007. Mealybug attack affects cotton crop on 150,000 acres. Journal of Applied Entomology 3, 35-42.

Karso, B.A., Al Mallah, N.M., 2014. Effect of mixing ratio and oil kind on toxicity activation of Acetamprid against Trogoderma granarium larvae. IOSR Journal of Pharmacy 4, 35-40.

Mark, P.C., Gullan, P.J., 2005. A new pest of tomato and other records of mealybugs (Hemiptera: Pseudococcidae) from Espírito Santo, Brazil. Zootaxa 964, 1-8.

McKenzie, H.L., 1967. Mealybugs of California with Taxonomy, Biology and Control of North American Species (Homoptera: Coccoidea: Pseudococcidae), University of California Press, Berkeley.

Mo-Jian, H., Mo, J.H., 2003. Long tailed mealybug. Monographic Series-NSW- Agriculture. No. January. 2, 5.

Mohyuddin, A.I., Jilani, G., Khan, A.G., Hamza, A., Ahmed, I., Mahmood, Z., 1997. Integrated pest management of major cotton pests by conservation, redistribution and augmentation of natural enemies. Pakistan Journal of Zoology 29, 293-298.

Muhammad, A., 2007. Mealybug: Cotton Crop’s Worst Catastrophe.Centre for Agro- Informatics Research (CAIR), Pakistan.

Muhammad, W., Ammar, M., Mukhtar, T., Hamza, A.M., 2022. Comparative efficacy of some new chemistry foliar insecticides against cotton whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). Asian Journal of Agriculture and Biology 2022, DOI: 10.35495/ajab.32021.35402.35075.

Muthukrishnan, N., Manoharan, T., Thevan, P.S.T., Anbu, S., 2005. Evaluation of buprofezin for the management of grape mealy bug, Maconellicoccus hirsutus (Green). Journal of Entomological Research 29, 339-344.

Nagrare, V., Kranthi, S., Biradar, V.K., Zade, N.N., Sangode, V., Kakde, G., Shukla, R.M., Shivare, D., Khadi, B.M., Kranthi, K.R., 2009. Widespread infestation of the exotic mealybug species, Phenacoccus solenopsis (Tinsley)(Hemiptera: Pseudococcidae), on cotton in India. Bulletin of Entomological Research 99, 537-541.

Naqvi, K.M., 1976. Crop protection to boost up the cotton production. Seminar organised by ESSO Fert Co Ltd Pakistan.

Parvez, I., 2008. Weekly and fortnightly pest scouting reports for the years 2005 to 2008, Unpublished data with Directorate General, Pest Warning and Quality Control of Pesticides Punjab.

Pedigo, L.P., Rice, M.E., 2009. Entomology and pest management, 6th Edition ed. Prentice-Hall of India Pvt. Ltd. New Delhi.

Pruthi, H.S., Batra, H., 1960. Important fruit pests of North-West India. Bulletin of Indian Council for Agricultural Research No. 80.

Rinehold, J., Jenkins, J., 2006. Spray-tank adjuvants. In: PNW Insect Management Hand book. Available from (Accessed: May 2008).

Saeed, S., Ahmad, M., Ahmad, M., KWON, Y.J., 2007. Insecticidal control of the mealybug Phenacoccus gossypiphilous (Hemiptera: Pseudococcidae), a new pest of cotton in Pakistan. Entomological Research 37, 76-80.

Saini, R.K., Sharma, S.S.P., Rohilla, H.R., 2009. Mealybug, Phenacoccus solenopsis Tinsley and its survival in cotton ecosystem in Haryana, Proceedings of the National Symposium on Bt Cotton: Opportunities and Prospects, Central Institute of Cotton Research, Nagpur, pp. 17-19.

Shaban, A., Al Mallah, N.M., 1993. Pesticides Book. Mosul, Iraq.

Sun, Y.-P., Johnson, E.R., 1960. Synergistic and antagonistic actions of insecticide-synergist combinations and their mode of action. Journal of Agricultural and Food Chemistry 8, 261-266.

Suresh, S., Jothimani, R., Sivasubrmanian, P., Karuppuchamy, P., Samiyappan, R.,, Jonatha, E.I., 2010. Invasive mealybugs of Tamil Nadu and their management. Karnataka Journal of Agricultural Sciences 23, 6-9.

Tabachnick, B.G., Fidell, L.S., 2001. Using multivariate statistics: A guide to statistical technique, 4th ed. Allyn and Bancon, Mass, Tokyo, New York.

Vennila, S., Ramamurthy, V.V., Deshmukh, A., Pinjarkar, D.B., Agarwal, M., Pagar, P.C., Prasad, Y.G., Prabhakar, M., Kranthi, K.R., Bambawale, O.M., 2010. A treatise on mealybugs of Central Indian cotton production system, Technical bulletin, National Centre for Integrated Pest Management New Delhi, pp. 30-39.

Walton, V.M., Pringle, K.L., 2001. Evaluation of the effects of pesticides used on table grapes, on the mealybug parasite, Coccindoxenoides peregrinus (Timberlake) (Hymenoptera: Encyrtidae). South African Journal of Enology and Viticulture 22, 107-110.

Wang, Y.P., Wu, S.A., Zhang, R.Z., 2009. Pest risk analysis of a new invasive pest, Phenacoccus solenopsis, to China. Chinese Bulletin of Entomology 46, 101-106.

Yunus, M., Yousaf, M., Jilani, G., 1980. Insect and spider mite pests of cotton in Pak. Monogr. PL-480, Deptt. Entomol., Univ. Agric., Faisalabad 256.

Yunus, M., Yousuf, M., 1979. Insect and mite pests of cotton in Pakistan. Pakistan Journal of Agricultural Sciences 16, 67-71.

Zaka, S.M., Saeed, S., Bukhari, S.A., Baksh, E., 2006. Mealybug Phenacoccus solenopsis (Pseudococcidae: Homoptera) a novel pest of cotton in Pakistan, 34th SAARC Science Conference, University of Veterinary and Animal Sciences, Lahore, Pakistan, p. 32.

Zhang, A., Amalin, D., Shirali, S., Serrano, M.S., Franqui, R.A., Oliver, J.E., Klun, J.A., Aldrich, J.R., Meyerdirk, D.E., Lapointe, S.L., 2004. Sex pheromone of the pink hibiscus mealybug, Maconellicoccus hirsutus, contains an unusual cyclobutanoid monoterpene. Proceedings of the National Academy of Sciences 101, 9601-9606.



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