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Foliar application of ascorbic acid alleviates adverse effects of zinc toxicity in Pisum sativum L. plants.

Muhammad Asif Akram, Muhammad Sajid Aqeel Ahmad, Muhammad Rashid, Muhammad Waqar, Allah Nawaz Khan, Nosheen Noor Elahi, Atfa Iqbal, Muhammad Usama Latif, Muhammad Ahmad Muavia


The present study was designed to observe whether the hostile effects of zinc stress on Pisum sativum L. plants could be alleviated by exogenous application of ascorbic acid as a foliar spray and how far it regulates the plant antioxidant enzyme system. A pot experiment was conducted to study the effect of foliar application of ascorbic acid on morphological and antioxidants attributes of two Pisum sativum L. cultivars (Meteor and 2001-40) grown under zinc stress. Treatment of ZnSO4.7H2O (5 ppm and 10 ppm) through rooting medium and ascorbic acid (5 ppm and 10 ppm) as foliar spray were applied after three weeks of germination. In pea plants exposed to zinc stress, growth was significantly reduced in terms of shoot and root lengths, fresh and dry weights, leaf area and number of leaves per plant. However, foliar application of ascorbic acid (AsA) was able to alleviate the zinc induced and significantly improve the above growth traits. The chlorophyll a, b and carotenoids levels were decreased under zinc stress. However, these pigments were increased by follow up the application of AsA. The total soluble proteins were decreased in zinc stress. The foliar application of AsA and zinc increased the level of ascorbic acids. The enzymatic activities of catalase (CAT) and Peroxides (POD) were decreased under zinc and foliar application AsA. Ascorbic acid (5 ppm and 10 ppm) played significant role in the amelioration the zinc stress. Overall, cv. 2001-40 performed better morphological, biochemical and antioxidants attributes compared to cv. Meteor under zinc stress.


Keywords: Ascorbic acid; Chlorophyll; Enzyme activity, Protein, Zinc stress

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