Foliar application of ascorbic acid alleviates adverse effects of zinc toxicity in Pisum sativum L. plants.

Muhammad Asif Akram, Muhammad Sajid Aqeel Ahmad, Muhammad Rashid, Muhammad Waqar, Allah Nawaz Khan, Nosheen Noor Elahi, Atfa Iqbal, Muhammad Usama Latif, Muhammad Ahmad Muavia

Abstract


The present study was designed to observe whether the hostile effects of zinc stress on Pisum sativum L. plants could be alleviated by exogenous application of ascorbic acid as a foliar spray and how far it regulates the plant antioxidant enzyme system. A pot experiment was conducted to study the effect of foliar application of ascorbic acid on morphological and antioxidants attributes of two Pisum sativum L. cultivars (Meteor and 2001-40) grown under zinc stress. Treatment of ZnSO4.7H2O (5 ppm and 10 ppm) through rooting medium and ascorbic acid (5 ppm and 10 ppm) as foliar spray were applied after three weeks of germination. In pea plants exposed to zinc stress, growth was significantly reduced in terms of shoot and root lengths, fresh and dry weights, leaf area and number of leaves per plant. However, foliar application of ascorbic acid (AsA) was able to alleviate the zinc induced and significantly improve the above growth traits. The chlorophyll a, b and carotenoids levels were decreased under zinc stress. However, these pigments were increased by follow up the application of AsA. The total soluble proteins were decreased in zinc stress. The foliar application of AsA and zinc increased the level of ascorbic acids. The enzymatic activities of catalase (CAT) and Peroxides (POD) were decreased under zinc and foliar application AsA. Ascorbic acid (5 ppm and 10 ppm) played significant role in the amelioration the zinc stress. Overall, cv. 2001-40 performed better morphological, biochemical and antioxidants attributes compared to cv. Meteor under zinc stress.


Keywords


Keywords: Ascorbic acid; Chlorophyll; Enzyme activity, Protein, Zinc stress

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References


Ahmed, F., Baloch, D.M., Hassan, M.J., Ahmed, N., 2013. Role of plant growth regulators in improving oil quantity and quality of sunflower hybrids in drought stress. Biologia 59, 315-322.

Arnon, D.I., Allen, M.B., Whatley, F.R., 1956. Photosynthesis by isolated chloroplasts IV. General concept and comparison of three photochemical reactions. Biochimica et Biophysica Acta-General Subjects 20, 449-461.

Bell, F.G., Bullock, S.E.T., Hälbich, T.F.J., Lindsay, P., 2001. Environmental impacts associated with an abandoned mine in the Witbank Coalfield, South Africa. International Journal of Coal Geology 45, 195-216.

Bhat, T.A., Gupta, M., Ganai, M.A., Ahanger, R.A., Bhat, H.A., 2013. Yield, soil health and nutrient utilization of field pea (Pisum sativum L.) as affected by phosphorus and biofertilizers under subtropical conditions of Jammu. International Journal of Modern Plant and Animal Science 1, 1-8.

Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.

Chao, Y., Kao, C.H., 2010. Heat shock-induced ascorbic acid accumulation in leaves increases cadmium tolerance of rice (Oryza sativa L.) seedlings. Plant and Soil 336, 39-48.

Codina, J.C., Cazorla, F.M., Pérez‐García, A., de Vicente, A., 2000. Heavy metal toxicity and genotoxicity in water and sewage determined by microbiological methods. Environmental Toxicology and Chemistry 19, 1552-1558.

Doberman, A., Fairhurst, T., 2000. Rice: Nutriments Disorders and Nutriments Management, International Plant Nutrition Institute.

Ebbs, S.D., Kochian, L.V., 1997. Toxicity of zinc and copper to Brassica species: implications for phytoremediation. Journal of Environmental Quality 26, 776-781.

Ghafoor, A., Ahmad, Z., Anwar, R., 2005. Genetic diversity in Pisum sativum and a strategy for indigenous biodiversity conservation. Pakistan Journal of Botany 37, 71-77.

Gomes, M.P., Nogueira, M.O.G., Castro, E.M., Soares, Â.M., 2011. Ecophysiological and anatomical changes due to uptake and accumulation of heavy metal in Brachiaria decumbens. Scientia Agricola 68, 566-573.

Luo, Z., He, X., Chen, L., Tang, L.n., Gao, S., Chen, F., 2010. Effects of zinc on growth and antioxidant responses in Jatropha curcas seedlings. International Journal of Agricultural Biology 12, 119-124.

Maehly, A., Chance, B., 1955. Assay of catalases and peroxidases, in methods in enzymology. Methods in Enzymology 2, 764-775.

Mukherjee, S.P., Choudhuri, M.A., 1983. Implication of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogenperoxide in Vigna seedlings. Physiologia Plantarum 58, 166-170.

Naz, F., Gul, H., Hamayun, M., Sayyed, A., Khan, H., Sherwani, S., 2014. Effect of NaCl stress on Pisum sativum germination and seedling growth with the influence of seed priming with potassium (KCL and KOH). American-Eurasian Journal of Agricultural and Environmental Sciences 14, 1304-1311.

Passariello, B., Giuliano, V., Quaresima, S., Barbaro, M., Caroli, S., Forte, G., Carelli, G., Iavicoli, I., 2002. Evaluation of the environmental contamination at an abandoned mining site. Microchemical Journal 73, 245-250.

Pastori, G.M., Kiddle, G., Antoniw, J., Bernard, S., Veljovic-Jovanovi, S., Verrier, P.J., Noctor, G., Foyer, C.H., 2003. Leaf vitamin C contents modulate plant defense transcripts and regulate genes that control development through hormone signaling. The Plant Cell 15, 939-951.

Pignocchi, C., Foyer, C.H., 2003. Apoplastic ascorbate metabolism and its role in the regulation of cell signalling. Current Opinion in Plant Biology 6, 379-389.

Schwartz, C., Gérard, E., Perronnet, K., Morel, J.L., 2001. Measurement of in situ phytoextraction of zinc by spontaneous metallophytes growing on a former smelter site. Science of the Total Environment 279, 215-221.

Shafiq, S., Akram, N.A., Ashraf, M., Arshad, A., 2014. Synergistic effects of drought and ascorbic acid on growth, mineral nutrients and oxidative defense system in canola (Brassica napus L.) plants. Acta Physiologiae Plantarum 36, 1539-1553.

Shalata, A., Neumann, P.M., 2001. Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. Journal of Experimental Botany 52, 2207-2211.

Sinniah, U.R., 2004. The Effect of different water regimes on yield and bioavailability of phosphorus in rice production in Malaysia Malaysian Journal of Soil Science 8, 53-62.

Smirnoff, N., Conklin, P.L., Loewus, F.A., 2001. Biosynthesis of ascorbic acid in plants: a renaissance. Annual Review of Plant Biology 52, 437-467.

Subba, P., Mukhopadhyay, M., Mahato, S.K., Bhutia, K.D., Mondal, T.K., Ghosh, S.K., 2014. Zinc stress induces physiological, ultra-structural and biochemical changes in mandarin orange (Citrus reticulata Blanco) seedlings. Physiology and Molecular Biology of Plants 20, 461-473.

Timmerman-Vaughan, G.M., Mills, A., Whitfield, C., Frew, T., Butler, R., Murray, S., Lakeman, M., McCallum, J., Russell, A., Wilson, D., 2005. Linkage mapping of QTL for seed yield, yield components, and developmental traits in pea. Crop Science 45, 1336-1344.

Vijayarengan, P., Mahalakshmi, G., 2013. Zinc toxicity in tomato plants. World Applied Sciences Journal 24, 649-653.


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