Email this article 
Hide
Show all
Evaluation of Genetic Diversity in Barley (Hordeum vulgare) Genotypes using Protein Profiling
Abstract
The knowledge of the genetic diversity of barley (Hordeum vulgare) genotypes based on protein polymorphism is very important for breeding programs. The purpose of the current study was to determine the genetic diversity and relationships among ten barley genotypes by using Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for protein profiles. A total number of 30 bands with molecular weights ranging from 12 to 148 KD were detected. Out of these, five bands were observed monomorphic. Rest of the bands had shown polymorphism to the extent of 83.3% among the test genotypes. The genetic similarity of the ten genotypes tested varied from 0.26 to 1.00 with an average of 0.51. Cluster analysis divided the ten genotypes into two major clusters comprising four subclusters, which was consistent with the systematic classification of barley done in previous studies. The results of this study indicated that the genotypes of barley could effectively be differentiated based on polymorphism, detected between protein patterns. SDS-PAGE presented a higher differentiation power and better repeatability; thus, could be used as a rapid and reliable method for genetic diversity analysis and laid a solid foundation for future barley breeding.
Keywords
References
Abou-Ellail, M., M. E.-S. Hattem, A. M. Mohamed, I. A. Usama, A. G. Hassan and A. E. E. 2014. Appraisal of biochemical and genetic diversity of mango cultivars using molecular markers. African Journal of Biotechnology, 13: 2796-2806.
Ahmed, M. F., M. Iqbal, M. Shahid Masood, M. Ashiq Rabbani and M. Munir. 2010. Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using rapd and sds-page. Electronic Journal of Biotechnology, 13.
Al-Hadeithi, Z. S., A. K. A. Al-Kazaz and B. K. Al-Obaidi. 2012. Genetic diversity and relationships among Iraqi barley cultivars using RAPD–PCR technique. The Iraqi Journal of Agricultural Science, 43: 117-124.
Ali, Z., A. S. Qureshi, W. Ali, H. Gulzar, M. Nisar and A. Ghafoor. 2007. Evaluation of genetic diversity present in pea (Pisum sativum L.) germplasm based on morphological traits, resistance to powdery mildew and molecular characteristics. Pakistan Journal of Botany, 39: 2739-2747.
Alipour, H., A. Rezai, S. A. M. Meibodi and M. Taheri. 2002. Evaluation of genetic variation in soybean lines using seed protein electrophoresis. JWSS-Isfahan University of Technology, 5: 85-96.
Aman, R. A. 1995. A comparative assessment of molecular techniques employed in genetic diversity studies (and their suitability in resource-limited settings). in: W.G. Ayad, T. Hodgkin, A. Jaradat & V.R. Rao (Eds.), IPGRI Workshop on Molecular Genetic Techniques for Plant Genetic Resources, Roma (Italia), 9-11 Oct 1995., p. 119-127.
Baenziger, P. S., D. R. Shelton, M. J. Shipman and R. A. Graybosch. 2001. Breeding for End-Use Quality: Reflections on the Nebraska Experience. Wheat in a Global Environment. Springer Netherlands, p. 255-262.
Baloch, A. W., M. Ali, A. M. Baloch, B. N. Mangan and W. Song. 2014. Genetic diversity and structure analysis based on hordein protein polymorphism in barley landrace populations from Jordan. Pakistan Journal of Botany, 46: 1397-1402.
Bohn, M., H. F. Utz and A. E. Melchinger. 1999. Genetic Similarities among Winter Wheat Cultivars Determined on the Basis of RFLPs, AFLPs, and SSRs and Their Use for Predicting Progeny Variance. Crop Science, 39: 228.
Bradford, M. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72: 248-254.
Chen, Z. W., R. J. Lu, L. Zou, Z. Z. Du, R. H. Gao, T. He and J. H. Huang. 2012. Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China. Genetics and Molecular Research, 11: 644-650.
Cooke, R. 1994. Variety Identification: Modcm Techniques and applications, In: A. Basra, (ed.), Seed quality: Basic mechanisms and agricultural implications. Haworth Press, New York, p. 279-317.
Dakir, E. L. H., M.-L. Ruiz, P. García and M. Pérez de la Vega. 2002. Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs. Genetic Resources and Crop Evolution, 49: 619-631.
Echart-Almeida, C. and S. Cavalli-Molina. 2000. Hordein variation in Brazilian barley varieties (Hordeum vulgare L.) and wild barley (H. euclaston Steud. and H. stenostachys Godr.). Genetics and Molecular Biology, 23: 425-433.
Feng, Z.-Y., X.-J. Liu, Y.-Z. Zhang and H.-Q. Ling. 2006. Genetic Diversity Analysis of Tibetan Wild Barley Using SSR Markers. Acta Genetica Sinica, 33: 917-928.
Gardiner, S. E. and M. B. Forde. 1992. Identification of Cultivars of Grasses and Forage Legumes by SDS-PAGE of Seed Proteins.
Modern Methods of Plant Analysis. Springer Berlin Heidelberg, p. 43-61.
Ghaderi, M., P. Vojdani and A. Gerami. 2003. Genetic diversity in the Iranian landraces of barley and its relation to geographical and climatic variation. Journal of Agricultural Science and Technology, 9: 50-70.
Gianibelli, M. C., R. B. Gupta, D. Lafiandra, B. Margiotta and F. MacRitchie. 2001. Polymorphism of High MrGlutenin Subunits in Triticum tauschii: Characterisation by Chromatography and Electrophoretic Methods. Journal of Cereal Science, 33: 39-52.
Heisel, S. E., D. M. Peterson and B. L. Jones. 1986. Identification of United States barley cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis of hordeins. Cereal Chemistry, 63: 500-505.
Javaid, A., A. Ghafoor and R. Anwar. 2004. Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pakistan Journal of Botany, 36: 25-30.
Jisha, V., L. Dampanaboina, J. Vadassery, A. Mithöfer, S. Kappara and R. Ramanan. 2015. Overexpression of an AP2/ERF Type Transcription Factor OsEREBP1 Confers Biotic and Abiotic Stress Tolerance in Rice. PloS One, 10: 0127831.
Karp, A. 1995. Molecular techniques in the analysis of the extent and distribution of genetic diversity, In: W.G. Ayad, T. Hodgkin, A. Jaradat & V.R. Rao, (eds.), Molecular Genetic Techniques for Plant Genetic Resources. International Plant Genetic Resource Institute, Rome, Italy, p. 11-22.
Karuppanapandian, T., T. Karuppudurai, P. B. Sinha, A. M. K. Haniya and K. Manoharan. 2006. Phylogenetic diversity and relationships among Cowpea (Vigna unguiculata L. Walp.) landraces using Random Amplified Polymor-Phic DNA markers. Plant Physiology, 32: 141-152.
Kuťka-Hlozákovás, T., E. Gregova, M. Vivodik and Z. Gálová. 2016. Genetic diversity of European cultivars of common wheat (Triticum aestivum L.) based on RAPD and protein markers. Journal of Central European Agriculture, 17: 957-969.
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
Lawrence, G. J. and K. W. Shepherd. 1980. Variation in Glutenin Protein Subunits of Wheat. Australian Journal of Biological Sciences, 33: 221.
Maris, A. 1992. Characterization isoenzyme of Brazilian cultivars of Hordeum vulgare. (Unpublished) Bachelor's thesis, University of Federal Rio Grand do Sul, Porto Alegre. RS.
Masoudi, B., M. R. Bihamta, H. R. Babaei and S. A. Peyghambari. 2008. Evaluation of genetic diversity for agronomic, morphological and phenological traits in soybean. Journal of Plant and Seed, 24: 413-427.
Masoumi, S. M., D. Kahrizi, H. Rostami-Ahmadvandi, J. Soorni, S. Kiani, A. Mostafaie and K. Yari. 2012. Genetic diversity study of some medicinal plant accessions belong to Apiaceae family based on seed storage proteins patterns. Molecular Biology Reports, 39: 10361-10365.
Mer, R. K., P. K. Prajith, D. H. Pandya and A. N. Pandey. 2000. Effect of Salts on Germination of Seeds and Growth of Young Plants of Hordeum vulgare, Triticum aestivum, Cicer arietinum and Brassica juncea. Journal of Agronomy and Crop Science, 185: 209-217.
Mzid, R., F. Chibani, R. B. Ayed, M. Hanana, J. Breidi, R. Kabalan, S. El-Hajj, H. Machlab, A. Rebai and L. Chalak. 2016. Genetic diversity in barley landraces (Hordeum vulgare L. subsp. vulgare) originated from Crescent Fertile region as detected by seed storage proteins. Journal of Genetics, 95: 733-739.
Pe´rez de, M. 1993. Biochemical characterization of populations, In: H. M.D., B. N.O. & R. I., (eds.), Plant Breeding - Principles and Prospects. Chapman & Hall, London, UK, p. 184-200.
Pournosrat, R., S. Kaya, S. Shaaf, B. Kilian and H. Ozkan. 2018. Geographical and environmental determinants of the genetic structure of wild barley in southeastern Anatolia. PloS one, 13: 0192386.
Radwan, S. A., A. S. El-Koly and R. H. Sammour. 2013. Genetic variation among accessions of Lathyrus inconspicuous (L.) as revealed by SDS Polyacrylamide Gel Electrophoresis / Analiza genetske variabilnosti akcesij grahorja (Lathyrus inconspicuous (L.) s SDS poliakrilamidno gelsko elektroforezo. Acta agriculturae Slovenica, 101: 21-30.
Redden, B., N. Maxted, B. Furman and C. Coyne. 2007. Lens biodiversity, In: S.S. Yadav, D.L. McNeil & P.C. Stevenson, (eds.), Lentil an ancient crop for modern times. Springer, Netherlands, p. 10-22.
Rohlf, F. J. 1987. NTSYS-pc: Microcomputer Programs for Numerical Taxonomy and Multivariate Analysis. The American Statistician, 41: 330.
Sadia, M., S. A. Malik, M. A. Rabbani and S. R. Pearce. 2009. Electrophoretic characterization and the relationship between some Brassica species. Electronic Journal of Biology, 5: 1-4.
Saisho, D. and K. Takeda. 2011. Barley: Emergence as a New Research Material of Crop Science. Plant and Cell Physiology, 52: 724-727.
Sharma, D. B. and K. Krishna. 2017. Genetic Diversity in Cowpea (Vigna unguiculata (L.) Walp.) Accessions using Protein Profiling. International Journal of Pure & Applied Bioscience, 5: 491-496.
Shekhghasemi, A. R., R. Shahryari and V. Mollasadeghi. 2013. Analysis of genetic diversity in barley genotypes using storage proteins. International Journal of Farming and Allied Sciences, 2: 448-453.
Shewry, P. R. 1995. Plant Storage Proteins. Biological Reviews, 70: 375-426.
Shuaib, M., I. Khan, Z. Ali, W. Ali, T. Ahmad and A. Zeb. 2007. Evaluation of Different Wheat Varieties by SDS-PAGE Electrophoresis. Pakistan Journal of Biological Sciences, 10: 1667-1672.
Tripathy, S. K., P. Mohanty, M. Jena, G. B. Dash, K. Pradhan, P. K. Nayak, S. Dash, D. Lenka, D. Mishra, P. M. Mohapatra, D. Swain and N. Senapati. 2016. Revealing contrasting genetic variation and study of genetic diversity in urdbean (Vigna mungo (L.) Hepper) using SDS-PAGE of seed storage proteins. Research in Biotechnology, 7.
Vargas, E., G. Macaya, J. Baudoin and O. Rocha. 2001. Case studies on breeding systems and its consequences for germplasm conservation: 3. Electrophoretic mobility of phaseolins in wild populations of Lima beans (Phaseolus lunatus L.) in the Central Valley of Costa Rica. Genetic Resources and Crop Evolution, 48: 109-120.
Zhang, D. and Y. Ding. 2007. Genetic diversity of wild close relatives of barley in Tibet of China revealed by AFLP. Hereditas, 29: 725-730.
DOI: https://doi.org/10.33687/pbg.006.01.2449
Refbacks
- There are currently no refbacks.
Copyright (c) 2018 Manal Eid
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Journal of Plant Breeding and Genetics
ISSN: 2305-297X (Online), 2308-121X (Print)
© EScience Press. All Rights Reserved.