ASSESMENT OF BIOCHEMICAL ATTRIBUTES OF PRAECITRULLUS FISTULOSUS TREATED WITH MUTAGENS

Mehreen Khan

Abstract


Plants are well known to have certain primary and secondary metabolites collectively are known as biochemicals that plays an important role for human health as their medicinal properties. The aim of present study was to enhance and evaluate biochemical profile of Praecitrullus fistulosus by induced mutagenesis to cause genetic variations, plant leaves were treated with different chemical and physical mutagens. Colchicine and Ethidium bromide were used as chemical mutagens. While Ultraviolet (UV) rays and X- rays were used as physical mutagens for the treatment of seeds. After the eleventh week of their growth, methanol extracts of dried leaves were prepared and further analyzed for the estimation of biochemicals. It was observed that total carbohydrates, total Proteins, phenolic compounds, antioxidant activity, reducing power, ascorbic Acid  and Chlorophyll a, were found significantly (p<0.05) higher in Colchicine 0.02% treated plants, while reducing sugars were significantly (p<0.05)  increases in Colchicine 0.01% treated plants as compared to control plants. Total flavonoids, total flavonol, Chlorophyll b and Carotenoids were increases significantly (p<0.05) in plants treated with 0.05% Ethidium bromide while tannin content was increased significantly (p<0.05) in 0.10% Ethidium bromide treated plants as compared to the  control plants.

Keywords


Biochemical attributes, Praecitrullus Fistulosus, mutagens

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