Morphophysiological and phenological responses of wheat genotypes to zinc oxide nanoparticles under drought stress
Abstract
Drought at grain filling is a major limitation for wheat production in arid and semi-arid regions. Zinc oxide nanoparticles (ZnO NPs) have been proposed as a tool to improve drought tolerance, but their performance across different wheat genotypes and water regimes is still unclear. In this pot experiment, five wheat lines (MS-1-14c-67, MS-13-17C-210, MS-14-14C-172, MS-15-17C-139 and MS-16-14C-143) were exposed at the grain filling stage to six treatments: well-watered control (T0), terminal drought (T1), 10 and 20 ppm ZnO under well-watered conditions (T2, T3), and 10 and 20 ppm ZnO combined with drought (T4, T5). Morphological, phenological and physiological traits, including biomass components, grain yield, grain filling percentage, relative water content (RWC), membrane stability index (MSI) and gas exchange parameters, were recorded and analyzed using two-way ANOVA. Drought alone significantly reduced shoot and spike biomass, thousand grain weight, RWC and MSI, and shortened the grain filling period from 43.7 to 28.1% of the crop life cycle. Foliar ZnO under non-stress (T2, T3) enhanced biomass, increased net photosynthesis above the control and raised MSI, with 10 ppm ZnO producing the highest mean grain yield and 20 ppm ZnO giving the greatest MSI. Under drought, ZnO partly restored RWC (to around 78% in ZnO treatments) and MSI and increased grain filling to 50–52% of the life cycle, indicating improved use of the post-anthesis period. Across treatments, MS-14-14C-172 and MS-16-14C-143 showed the highest biomass and yield with relatively stable physiological status, whereas MS-1-14c-67 produced lower biomass but maintained higher pigment and reserve levels. Overall, moderate foliar doses of ZnO NPs alleviated terminal drought mainly by supporting tissue water status, membrane integrity and grain filling, and identified MS-14-14C-172 and MS-16-14C-143 as promising lines for ZnO-mediated drought management in wheat.
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Arzani, A., & Ashraf, M. (2017). Cultivated ancient wheats (Triticum spp.): A potential source of health‐beneficial food products. Comprehensive Reviews in Food Science and Food Safety, 16(3), 477-488.
Balusamy, S. R., Joshi, A. S., Perumalsamy, H., Mijakovic, I., & Singh, P. (2023). Advancing sustainable agriculture: a critical review of smart and eco-friendly nanomaterial applications. Journal of Nanobiotechnology, 21(1), 372.
Benjamin, J., Idowu, O., Babalola, O. K., Oziegbe, E. V., Oyedokun, D. O., Raheem, J., Omole, A., Hussayn, A.-Q., Akinyemi, A. M., & Adebayo, A. (2025). Status of cereal production in Africa: insights from abiotic stressors in a changing climate—a short review. Discover Agriculture, 3(1), 115.
Czarnek, K., Tatarczak-Michalewska, M., Dreher, P., Rajput, V. D., Wójcik, G., Gierut-Kot, A., Szopa, A., & Blicharska, E. (2023). UV-C seed surface sterilization and Fe, Zn, Mg, Cr biofortification of wheat sprouts as an effective strategy of bioelement supplementation. International Journal of Molecular Sciences, 24(12), 10367.
El-Saadony, M. T., Saad, A. M., Soliman, S. M., Salem, H. M., Desoky, E.-S. M., Babalghith, A. O., El-Tahan, A. M., Ibrahim, O. M., Ebrahim, A. A., & Abd El-Mageed, T. A. (2022). Role of nanoparticles in enhancing crop tolerance to abiotic stress: A comprehensive review. Frontiers in Plant Science, 13, 946717.
El-Sharkawy, M., Mahmoud, E., Abd El-Aziz, M., & Khalifa, T. (2022). Effect of zinc oxide nanoparticles and soil amendments on wheat yield, physiological attributes and soil properties grown in the saline–sodic soil. Communications in Soil Science and Plant Analysis, 53(17), 2170-2186.
Fahad, S., Bajwa, A. A., Nazir, U., Anjum, S. A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., & Saud, S. (2017). Crop production under drought and heat stress: plant responses and management options. Frontiers in Plant Science, 8, 1147.
Fatima, A., Safdar, N., Ain, N.-u., Yasmin, A., & Chaudhry, G.-e.-S. (2023). Abscisic acid-loaded ZnO nanoparticles as drought tolerance inducers in Zea mays L. with physiological and biochemical attributes. Journal of Plant Growth Regulation, 42(11), 7280-7293.
Gupta, P., Balyan, H., Sharma, S., & Kumar, R. (2021). Biofortification and bioavailability of Zn, Fe and Se in wheat: present status and future prospects. Theoretical and Applied Genetics, 134(1), 1-35.
Indu Sharma, I. S., Tyagi, B., Gyanendra Singh, G. S., Venkatesh, K., & Gupta, O. (2015). Enhancing wheat production-a global perspective.
Jasrotia, P., Kashyap, P. L., Bhardwaj, A. K., Kumar, S., & Singh, G. P. (2018). Scope and applications of nanotechnology for wheat production: A review of recent advances. Wheat Barley Res, 10(1), 1-14.
Kansotia, K., Naresh, R., Sharma, Y., Sekhar, M., Sachan, P., Baral, K., & Pandey, S. K. (2024). Nanotechnology-driven solutions: Transforming agriculture for a sustainable and productive future. Journal of Scientific Research and Reports, 30(3), 32-51.
Khan, Z. S., Rizwan, M., Hafeez, M., Ali, S., Javed, M. R., & Adrees, M. (2019). The accumulation of cadmium in wheat (Triticum aestivum) as influenced by zinc oxide nanoparticles and soil moisture conditions. Environmental Science and Pollution Research, 26(19), 19859-19870.
Krishnappa, G., Khan, H., Krishna, H., Kumar, S., Mishra, C. N., Parkash, O., Devate, N. B., Nepolean, T., Rathan, N. D., & Mamrutha, H. M. (2022). Genetic dissection of grain iron and zinc, and thousand kernel weight in wheat (Triticum aestivum L.) using genome-wide association study. Scientific Reports, 12(1), 12444.
Li, Y., Liang, L., Li, W., Ashraf, U., Ma, L., Tang, X., Pan, S., Tian, H., & Mo, Z. (2021). ZnO nanoparticle-based seed priming modulates early growth and enhances physio-biochemical and metabolic profiles of fragrant rice against cadmium toxicity. Journal of Nanobiotechnology, 19(1), 75.
Liaqat, W., Altaf, M. T., Barutçular, C., Mohamed, H. I., Ahmad, H., Jan, M. F., & Khan, E. H. (2024). Sorghum: a star crop to combat abiotic stresses, food insecurity, and hunger under a changing climate: a review. Journal of Soil Science and Plant Nutrition, 24(1), 74-101.
Mahajan, A., Bhagat, R., & Gupta, R. (2008). Integrated nutrient management in sustainable rice-wheat cropping system for food security in India. SAARC Journal of Agriculture, 6(2), 29-32.
Melash, A. A., Bogale, A. A., Bytyqi, B., Nyandi, M. S., & Ábrahám, É. B. (2023). Nutrient management: As a panacea to improve the caryopsis quality and yield potential of durum wheat (Triticum turgidum L.) under the changing climatic conditions. Frontiers in Plant Science, 14, 1232675.
Pandya, P., Kumar, S., Sakure, A. A., Rafaliya, R., & Patil, G. B. (2023). Zinc oxide nanopriming elevates wheat drought tolerance by inducing stress-responsive genes and physio-biochemical changes. Current Plant Biology, 35, 100292.
Paul, K., Pauk, J., Kondic-Spika, A., Grausgruber, H., Allahverdiyev, T., Sass, L., & Vass, I. (2019). Co-occurrence of mild salinity and drought synergistically enhances biomass and grain retardation in wheat. Frontiers in Plant Science, 10, 501.
Rao, A., Ahmad, S., Sabir, S., Awan, S., Hameed, A., Abbas, S., Shehzad, M., Khan, M., Shafique, S., & Ahmad, Z. (2013). Detection of saline tolerant wheat cultivars (Triticum aestivum L.) using lipid peroxidation, antioxidant defense system, glycinebetaine and proline contents. J Anim Plant Sci, 23, 1742-1748.
Raza, M. A. S., Zulfiqar, B., Iqbal, R., Muzamil, M. N., Aslam, M. U., Muhammad, F., Amin, J., Aslam, H. M. U., Ibrahim, M. A., & Uzair, M. (2023). Morpho-physiological and biochemical response of wheat to various treatments of silicon nano-particles under drought stress conditions. Scientific Reports, 13(1), 2700.
Rehman, S. U., Bilal, M., Rana, R. M., Tahir, M. N., Shah, M. K. N., Ayalew, H., & Yan, G. (2016). Cell membrane stability and chlorophyll content variation in wheat (Triticum aestivum) genotypes under conditions of heat and drought. Crop and Pasture Science, 67(7), 712-718.
Sadati, S. Y. R., Godehkahriz, S. J., Ebadi, A., & Sedghi, M. (2022). Zinc oxide nanoparticles enhance drought tolerance in wheat via physio-biochemical changes and stress genes expression. Iranian Journal of biotechnology, 20(1), e3027.
Shewry, P. R. (2009). Wheat. Journal of experimental botany, 60(6), 1537-1553.
Shiferaw, B., Prasanna, B. M., Hellin, J., & Bänziger, M. (2011). Crops that feed the world 6. Past successes and future challenges to the role played by maize in global food security. Food security, 3(3), 307-327.
Singh, S., Kaur, J., Ram, H., Singh, J., & Kaur, S. (2023). Agronomic bio-fortification of wheat (Triticum aestivum L.) to alleviate zinc deficiency in human being. Reviews in Environmental Science and Bio/Technology, 22(2), 505-526.
Singh, S. K., Barman, M., Sil, A., Prasad, J. P., Kundu, S., & Bahuguna, R. N. (2023). Nutrient biofortification in wheat: opportunities and challenges. Cereal Research Communications, 51(1), 15-28.
Szerement, J., Szatanik-Kloc, A., Mokrzycki, J., & Mierzwa-Hersztek, M. (2022). Agronomic biofortification with Se, Zn, and Fe: An effective strategy to enhance crop nutritional quality and stress defense—A review. Journal of Soil Science and Plant Nutrition, 22(1), 1129-1159.
Ullah, A., Nadeem, F., Nawaz, A., Siddique, K. H., & Farooq, M. (2022). Heat stress effects on the reproductive physiology and yield of wheat. Journal of agronomy and crop science, 208(1), 1-17.
Wakeham, P. (2013). The medicinal and pharmacological screening of wheatgrass juice (Triticum aestivum L.): an investigation into chlorophyll content and antimicrobial activity. The Plymouth Student Scientist, 6(2), 20-30.
Waterborg, J. H. (2009). The Lowry method for protein quantitation. The protein protocols handbook, 7-10.
Yasin Goa Chondie, Y. G. C. (2015). Effect of integrated nutrient management on wheat: a review.
DOI: https://doi.org/10.33804/pp.010.02.5980
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