Toxicity and repellent effects of acetonic plant extracts against the lesser grain borer, Rhyzopertha dominica
Abstract
Stored grain pests cause considerable damage by feeding on stored commodities, resulting in significant qualitative and quantitative losses. Although chemical pesticides are widely used for protection, their effectiveness has been compromised by toxic residues in food, a limited range of approved compounds, and the development of resistant pest populations. Therefore, there is an urgent need for safer and more sustainable alternatives. The present study, therefore, evaluated the insecticidal and repellent efficacy of five acetonic plant extracts viz., Ruta chalepensis, Artemisia judaica, Heliotropium bacciferum, Salvadora persica, and Moringa oleifera against the lesser grain borer, Rhyzopertha dominica F. (Coleoptera: Bostrichidae). Dose-response bioassays were conducted to determine adult mortality, LC50 values, and repellence at various concentrations and exposure durations. An increase in extract concentration led to higher mortality and toxicity, showing clear dose- and time-dependent effects. Among the tested plants, A. judaica and R. chalepensis were the most effective, exhibiting the lowest LC50 values and strongest repellence. Acetonic extracts of A. judaica, R. chalepensis, and S. persica demonstrated particularly significant insecticidal and repellent activity against R. dominica adults. These findings highlight the potential of plant-derived extracts as eco-friendly alternatives to conventional synthetic pesticides. Their biodegradability, low residue levels, and reduced ecological risks make them promising candidates for incorporation into integrated pest management strategies, particularly in the context of rising insecticide resistance and growing environmental and health concerns associated with chemical control.
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Abbott, W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265-267.
Abdellaoui, K., Acheuk, F., Miladi, M., Boughattas, I., Omri, G., 2018. Phytochemistry, biochemical and insecticidal activities of Ruta chalepensis essential oils on Tribolium confusum. Agriculture and Forestry 64, 31-45.
Abdel-Sattar, E.A., Zaitoun, A.M.A., Farag, M.A., El Gayed, S.H., Harraz, F.M., 2010. Chemical composition, insecticidal and insect repellent activity of Schinus molle L. leaf and fruit essential oils against Trogoderma granarium and Tribolium castaneum. Natural Product Research 24, 226-235.
Abubakar, R., Suleiman, M., Wagini, N.H., 2021. Phytochemical and insecticidal properties of some botanical extracts against the lesser grain borer, Rhyzopertha dominica F. (Coleoptera: Bostrichidae). Journal of Entomology and Zoology Studies 9, 1-7.
Ahmady, A., Moahid, A.J., Mukhles, M.R., Andar, F., Ahmadi, A.J., 2024. Efficacy of some plant extracts against stored-product pest red flour beetle, Tribolium confusum (Jacquelin Du Val) (Coleoptera: Tenebrionidae) adults. International Journal of Current Science Research and Review 7, 1-8.
Akinkurolere, R.O., Adedire, C.O., Odeyemi, O.O., 2006. Laboratory evaluation of the toxic properties of forest anchomanes, Anchomanes difformis against pulse beetle Callosobruchus maculatus (Coleoptera: Bruchidae). Insect Science 13, 25-29.
Albeshri, A., Baeshen, N.A., Bouback, T., Aljaddawi, A.A., 2021. A review of Rhazya stricta Decne phytochemistry, bioactivities, pharmacological activities, toxicity, and folkloric medicinal uses. Plants 10, 1-25.
Ali, B.H., Al-Qarawi, A.A., Bashir, A.K., Tanira, M.O.M., 2000. Phytochemistry, pharmacology and toxicity of Rhazya stricta Decne: A review. Phytotherapy Research 14, 229-234.
Alvi, A.M., Iqbal, N., Bashir, M.A., Rehmani, M.I.A., Ullah, Z., Latif, A., Saeed, Q., 2018. Efficacy of Rhazya stricta leaf and seed extracts against Rhyzopertha dominica and Trogoderma granarium. Kuwait Journal of Science 45, 1-8.
Arthur, F.H., 1996. Grain protectants: Current status and prospects for the future. Journal of Stored Products Research 32, 293-302.
Arthur, F.H., Ondier, G.O., Siebenmorgen, T.J., 2012. Impact of Rhyzopertha dominica (F.) on quality parameters of milled rice. Journal of Stored Products Research 48, 137-142.
Asiry, K.A., Almasoudi, N.M., Zaitoun, A.A., 2022. Insecticidal and repellent actions of methanolic extracts from five medicinal plants against the saw-toothed grain beetle Oryzaephilus surinamensis (Linn.) (Coleoptera: Silvanidae). Revista de la Sociedad Entomologica Argentina 81, 41-51.
Bougherra, H.H., Bedini, S., Flamini, G., Cosci, F., Belhamel, K., Conti, B., 2015. Pistacia lentiscus essential oil has repellent effect against three major insect pests of pasta. Industrial Crops and Products 63, 249-255.
Collins, P.J., Daglish, G.J., Bengston, M., Lambkin, T.M., Pavic, H., 2002. Genetics of resistance to phosphine in Rhyzopertha dominica (Coleoptera: Bostrichidae). Journal of Economic Entomology 95, 862-869.
Daglish, G.J., Jagadeesan, R., Burrill, P.R., May, P.D., Wade, A.J., Nayak, M.K., 2024. Potential of flavesone as a grain protectant: Long-term efficacy and residues for controlling the lesser grain borer, Rhyzopertha dominica (F.), in stored wheat. Journal of Stored Products Research 109, 102467.
Deshwal, R., Gupta, P.K., Vaibhav, V., Kumar, N., Kumar, A., 2018. Biology of lesser grain borer (Rhyzopertha dominica Fab.), under different temperature and humidity at laboratory condition. Journal of Entomology and Zoology Studies 6, 364-368.
Edde, P.A., 2012. A review of the biology and control of Rhyzopertha dominica (F.) the lesser grain borer. Journal of Stored Products Research 48, 1-18.
Eldidamony, A.M., Moustafa, G.G., Mead, H.M.I., El-Shafiey, S.N., Abdelhafez, A., 2020. New insight on Heliotropium curassavicum L. extracts as a rodenticide. Journal of Environmental Science and Toxicology 14, 1-10.
Fields, P.G., White, N.D.G., 2002. Alternatives to methyl bromide treatments for stored-product and quarantine insects. Annual Review of Entomology 47, 331-359.
Finney, D.J., 1971. Probit analysis, third ed. Cambridge University Press, Cambridge, UK.
Frank, O.O., Agu, C.M., Ahuchaogu, C.E., 2013. Potentiality of Moringa oleifera Lam. extracts in the control of some field-store insect pests of cowpea. International Journal of Agricultural Science 4, 3537-3542.
Guru, P.N., Mridula, D., Dukare, A.S., Ghodki, B.M., Paschapur, A.U., Samal, I., Nikhil Raj, M., Padala, V.K., Rajashekhar, M., Subbanna, A.R.N.S., 2022. A comprehensive review on advances in storage pest management: Current scenario and future prospects. Frontiers in Sustainable Food Systems 6, 1-20.
Hamel, D., Rozman, V., Liška, A., 2020. Storage of cereals in warehouses with or without pesticides. Insects 11, 1-16.
Jbilou, R., Ennabili, A., Sayah, F., 2006. Insecticidal activity of four medicinal plant extracts against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). African Journal of Biotechnology 5, 936-940.
Jilani, G., Saxena, R.C., Rueda, B.P., 1988. Repellent and growth-inhibiting effects of turmeric oil, sweetflag oil, neem oil, and “Margosan-O” on red flour beetle (Coleoptera: Tenebrionidae). Journal of Economic Entomology 81, 1226-1230.
Kavallieratos, N.G., Athanassiou, C.G., Arthur, F.H., Throne, J.E., 2012. Lesser grain borers, Rhyzopertha dominica, select rough rice kernels with cracked hulls for reproduction. Journal of Insect Science 12, 38.
Kundu, B.R., Ara, R., Begum, M.M., Sarker, Z.I., 2007. Effect of Bishkatali, Polygonum hydropiper L. plant extracts against the red flour beetle, Tribolium castaneum Herbst. University Journal of Zoology, Rajshahi University 26, 93-97.
Leskovac, A., Petrović, S., 2023. Pesticide use and degradation strategies: Food safety, challenges and perspectives. Foods 12, 1-17.
Lorini, I., Collins, P.J., Daglish, G.J., Nayak, M.K., Pavic, H., 2007. Detection and characterisation of strong resistance to phosphine in Brazilian Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae). Pest Management Science 63, 358-364.
Mahmoud, A.K., Bedawi, S.M., Satti, A.A., 2015. Efficacy of some botanical extracts in the control of khapra beetle (Trogoderma granarium). Journal of Science 5, 213-217.
Mantzoukas, S., Lagogiannis, I., Kitsiou, F., Eliopoulos, P.A., 2023. Entomopathogenic action of wild fungal strains against stored product beetle pests. Insects 14, 1-11.
Martins, C.H.Z., Freir, M.G.M., Parra, J.R.P., Macedo, M.L.R., 2012. Physiological and biochemical effects of an aqueous extract of Koelreuteria paniculata (Laxm.) seeds on Anticarsia (Lepidoptera: Noctuidae). Brazilian Journal of Entomology 56, 81-93.
Marwat, S.K., Fazal-ur-Rehman, Usman, K., Shah, S.S., Anwar, N., Ullah, I., 2012. A review of phytochemistry, bioactivities and ethnomedicinal uses of Rhazya stricta Decne (Apocynaceae). African Journal of Microbiology Research 6, 1629-1641.
Meikle, W.G., Cherry, A.J., Holst, N., Hounna, B., Markham, R.H., 2001. The effects of an entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin (Hyphomycetes), on Prostephanus truncatus (Horn) (Col.: Bostrichidae), Sitophilus zeamais Motschulsky (Col.: Curculionidae), and grain losses in stored maize in the Benin Republic. Journal of Invertebrate Pathology 77, 198-205.
Nazurah, R.N.F., Noranizan, M.A., Nor-Khaizura, M.A.R., Nur Hanani, Z.A., 2022. The potential of chitosan-based film with curry leaf essential oil as natural insect-repellent food packaging. Food Packaging and Shelf Life 34, 100993.
Onyilagha, J.C., Lazorko, J., Gruber, M.Y., Soroka, J.J., Erlandson, M.A., 2004. Effect of flavonoids on feeding preference and development of the crucifer pest Mamestra configurata Walker. Journal of Chemical Ecology 30, 109-124.
Parichanon, P., Ascrizzi, R., Echeverría, M.C., Farina, P., Pieracci, Y., Flamini, G., Semprucci, F., Guidi, L., Grassi, E., Gogou, T.I., Ortega-Andrade, S., Conti, B., 2025. The aromatic plant essential oils and their hormetic effect on Rhyzopertha dominica (Coleoptera: Bostrichidae). Crop Protection 194, 107235.
Rajendran, S., Sriranjini, V., 2008. Plant products as fumigants for stored-product insect control. Journal of Stored Products Research 44, 126-135.
Sarwar, H., 2013. The importance of cereals (Poaceae: Gramineae) nutrition in human health: A review. Journal of Cereals and Oilseeds 4, 32-35.
Stathers, T.E., Žďárková, E., Wakefield, M., Lukas, J., Hubert, J., 2002. Entomopathogenic fungi in grain storage - any lessons for Europe from elsewhere? Journal of Stored Products Research 38, 305-313.
Sutherland, J.P., Baharally, V., Permaul, D., 2002. Use of the botanical insecticide, neem to control the small rice stinkbug Oebalus poecilus (Dallas, 1851) (Hemiptera: Pentatomidae) in Guyana. Entomotropica 17, 97-101.
Talukder, F.A., Howse, P.E., 1994. Laboratory evaluation of toxic and repellent properties of the pithraj tree, Aphanamixis polystachya Wall & Parker, against Sitophilus oryzae (L.). International Journal of Pest Management 40, 274-279.
Tapondjou, L.A., Adler, C., Bouda, H., Fontem, D.A., 2002. Efficacy of powder and essential oil from Chenopodium ambrosioides leaves as post-harvest grain protectants against six stored-product beetles. Journal of Stored Products Research 38, 395-402.
Upadhyay, R.K., 2013. Capparis decidua induced toxicity, biochemical and enzymatic alterations in Rhyzopertha dominica (Fabr.) (Coleoptera: Bostrichidae). International Journal of Biological Sciences 5, 1-12.
Yousif, R.E., Taha, A.K., 2016. Evaluation of some plant extracts against adults of the saw-toothed grain beetle, Oryzaephilus surinamensis (Linn.) (Coleoptera: Silvanidae). Universal Journal of Agricultural Research 4, 170-174.
Zettler, L.J., Cuperus, G.W., 1990. Pesticide resistance in Tribolium castaneum (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae) in wheat. Journal of Economic Entomology 83, 1677-1681.
Zibaee, A., Stoytcheva, M., 2011. Botanical insecticides and their effects on insect biochemistry and immunity. Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment 1, 55-68.
DOI: https://doi.org/10.33804/pp.009.04.5887
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